3.5.4.9: methenyltetrahydrofolate cyclohydrolase

This is an abbreviated version!
For detailed information about methenyltetrahydrofolate cyclohydrolase, go to the full flat file.

Word Map on EC 3.5.4.9

Reaction

5,10-methenyltetrahydrofolate
+
H2O
=
10-formyltetrahydrofolate

Synonyms

More, 5,10-Methenyltetrahydrofolate synthetase, Citrovorum factor cyclodehydrase, cyclohydrolase, formyl-methenyl-methylenetetrahydrofolate synthetase (combined), 5,10-methenyl-H4folate cyclohydrolase, 5,10-CH+-H4PteGlu cyclohydrolase, CHF4CH, mosquito cyclohydrolase, methenyl-THF cyclohydrolase, methenylH4F cyclohydrolase, bifunctional methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase, methyleneH4folate cyclohydrolase, methenyltetrahydrofolate dehydrogenase/cyclohydrolase, 5,10-CH=H4 folate synthetase, 5,10-methenyltetrahydrofolate cyclohydrolase, MTHFD1, methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase/formyltetrahydrofolate synthetase, 5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase, MTHFDC, DHCH1, methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase, methylene tetrahydrofolate dehydrogenase/cyclohydrolase, 5,10-methenyltetrahydrofolate cyclohydrolase/5,10-methylene tetrahydrofolate dehydrogenase, dehydrogenasse-cyclohydrolase-synthetase, ADE3, MIS1, MTHFD2, NAD-dependent methylenetetrahydrofolate dehydrogenase-cyclohydrolase, methenyltetrahydrofolate cyclohydrolase, methylene-tetrahydrofolate dehydrogenase/cyclohydrolase, DHCH 1, DHCH 2, methenyl-tetrahydrofolate cyclohydrolase, 5,10-methenyl-THF cyclohydrolase, methylenetetrahydrofolate dehydrogenase/cyclohydrolase, N5,N10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase, N5,N10-methylenetetrahydrofolate dehydrogenase/N5,N10-methenyltetrahydrofolate cyclohydrolase, DHCH, MTHFD2L, FolD, bifunctional N5,N10-methylene-H4F dehydrogenase/N5,N10-methenyl-H4F cyclohydrolase, methenyl-H4F cyclohydrolase, CH+-THF cyclohydrolase, N5,N10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase, methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase, FchA, 5,10-CH+-THF cyclohydrolase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.9 methenyltetrahydrofolate cyclohydrolase

Purification

Purification on EC 3.5.4.9 - methenyltetrahydrofolate cyclohydrolase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by anion-exchange chromatography
His-Trap affinity column chromatography and Superdex-S200 gel filtration
-
Ni2+-charged HisTrap column chromatography and Superdex 200 gel filtration
recombinant DHCH1 protein is purified by metal-affinity chromatography
-
recombinant His-tagged enzyme by nickel affinity chromatography, cleavage of the His-tag by TEV protease, His affinity chromatography, and gel filtration, to over 95% purity
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by Ni2+-chelate affinity chromatography
recombinant N-terminally 6-His-tagged MTHFDC from Escherichia coli strain BL21(DE3) by heat-treatment, nickel affinity chromatography, and gel filtration
recombinant protein expressed in Escherichia coli
-
recombinant protein expressed in Escherichia coli K38
-