KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
5,10-methenyltetrahydrofolate | - |
Clostridium formicaceticum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25500 | - |
2 * 25500, SDS-PAGE | Clostridium formicaceticum |
41000 | - |
sedimentation equilibrium centrifugation | Clostridium formicaceticum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate | Clostridium formicaceticum | - |
5,10-methenyltetrahydrofolate + H2O | - |
r | |
5,10-methenyltetrahydrofolate + H2O | Escherichia coli | - |
10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | Saccharomyces cerevisiae | - |
10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | Moorella thermoautotrophica | - |
10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | Moorella thermoacetica | - |
10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | Clostridium formicaceticum | - |
10-formyltetrahydrofolate | - |
r | |
5,10-methenyltetrahydrofolate + H2O | Acetobacterium woodii | - |
10-formyltetrahydrofolate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetobacterium woodii | - |
bifunctional enzyme | - |
Clostridium formicaceticum | - |
ATCC 23439, monofunctional enzyme | - |
Escherichia coli | - |
- |
- |
Moorella thermoacetica | - |
bifunctional enzyme | - |
Moorella thermoautotrophica | - |
bifunctional enzyme | - |
Saccharomyces cerevisiae | - |
yeast, trifunctional enzyme | - |
Purification (Comment) | Organism |
---|---|
- |
Clostridium formicaceticum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
469 | - |
- |
Clostridium formicaceticum |
470 | - |
purified enzyme, pH 7.2, 35°C | Clostridium formicaceticum |
Storage Stability | Organism |
---|---|
5°C, 50 mM phosphate buffer, pH 7.0, stable for at least 1 week | Clostridium formicaceticum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate | - |
Clostridium formicaceticum | 5,10-methenyltetrahydrofolate + H2O | - |
r | |
5,10-methenyltetrahydrofolate + H2O | - |
Escherichia coli | 10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | - |
Saccharomyces cerevisiae | 10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | - |
Moorella thermoautotrophica | 10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | - |
Moorella thermoacetica | 10-formyltetrahydrofolate | - |
? | |
5,10-methenyltetrahydrofolate + H2O | - |
Clostridium formicaceticum | 10-formyltetrahydrofolate | - |
r | |
5,10-methenyltetrahydrofolate + H2O | - |
Acetobacterium woodii | 10-formyltetrahydrofolate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Escherichia coli |
dimer | - |
Saccharomyces cerevisiae |
dimer | 2 * 25500, SDS-PAGE | Clostridium formicaceticum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
70% activity lost within 2 min, rapidly inactivated at temperatures above | Clostridium formicaceticum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | 7.6 | - |
Clostridium formicaceticum |
7 | - |
- |
Clostridium formicaceticum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.8 | 8 | - |
Clostridium formicaceticum |