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Literature summary for 3.5.4.9 extracted from

  • Kim, J.H.; Sung, M.W.; Lee, E.H.; Nam, K.H.; Hwang, K.Y.
    Crystallization and preliminary X-ray diffraction analysis of 5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase from Thermoplasma acidophilum DSM 1728 (2008), J. Microbiol. Biotechnol., 18, 283-286.
    View publication on PubMed
Search for more literature for 3.5.4.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21 (DE3) Thermoplasma acidophilum DSM 1728

Crystallization (Commentary)

Crystallization (Comment) Organism
in the presence of NADP+, at 22°C, using polyethylene glycol 4000 as a precipitant, sitting drop vapour diffusion method Thermoplasma acidophilum DSM 1728

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30600
-
 estimated from amino acid sequence Thermoplasma acidophilum DSM 1728

Organism

Organism UniProt Comment Textmining
Thermoplasma acidophilum DSM 1728
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap affinity column chromatography and Superdex-S200 gel filtration Thermoplasma acidophilum DSM 1728

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methenyltetrahydrofolate + H2O
-
 Thermoplasma acidophilum DSM 1728 10-formyltetrahydrofolate
-
 ?

Subunits

Subunits Comment Organism
dimer x-ray crystallography Thermoplasma acidophilum DSM 1728

Synonyms

Synonyms Comment Organism
5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase
-
 Thermoplasma acidophilum DSM 1728
MTHFDC
-
 Thermoplasma acidophilum DSM 1728

Cofactor

Cofactor Comment Organism Structure
additional information preference for NADP+ as a cofactor, rather than NAD+ Thermoplasma acidophilum DSM 1728