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3.5.4.9: methenyltetrahydrofolate cyclohydrolase

This is an abbreviated version!
For detailed information about methenyltetrahydrofolate cyclohydrolase, go to the full flat file.

Word Map on EC 3.5.4.9

Reaction

5,10-methenyltetrahydrofolate
+
H2O
=
10-formyltetrahydrofolate

Synonyms

5,10-CH+-H4PteGlu cyclohydrolase, 5,10-CH+-THF cyclohydrolase, 5,10-CH=H4 folate synthetase, 5,10-methenyl-H4folate cyclohydrolase, 5,10-methenyl-THF cyclohydrolase, 5,10-methenyltetrahydrofolate cyclohydrolase, 5,10-methenyltetrahydrofolate cyclohydrolase/5,10-methylene tetrahydrofolate dehydrogenase, 5,10-Methenyltetrahydrofolate synthetase, 5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase, ADE3, bifunctional methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase, bifunctional N5,N10-methylene-H4F dehydrogenase/N5,N10-methenyl-H4F cyclohydrolase, CH+-THF cyclohydrolase, CHF4CH, Citrovorum factor cyclodehydrase, cyclohydrolase, dehydrogenasse-cyclohydrolase-synthetase, DHCH, DHCH 1, DHCH 2, DHCH1, FchA, FolD, formyl-methenyl-methylenetetrahydrofolate synthetase (combined), methenyl-H4F cyclohydrolase, methenyl-tetrahydrofolate cyclohydrolase, methenyl-THF cyclohydrolase, methenylH4F cyclohydrolase, methenyltetrahydrofolate cyclohydrolase, methenyltetrahydrofolate dehydrogenase/cyclohydrolase, methylene tetrahydrofolate dehydrogenase/cyclohydrolase, methylene-tetrahydrofolate dehydrogenase/cyclohydrolase, methyleneH4folate cyclohydrolase, methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase, methylenetetrahydrofolate dehydrogenase/cyclohydrolase, methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase, methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase/formyltetrahydrofolate synthetase, MIS1, More, mosquito cyclohydrolase, MTHFD1, MTHFD2, MTHFD2L, MTHFDC, N5,N10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase, N5,N10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase, N5,N10-methylenetetrahydrofolate dehydrogenase/N5,N10-methenyltetrahydrofolate cyclohydrolase, NAD-dependent methylenetetrahydrofolate dehydrogenase-cyclohydrolase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.9 methenyltetrahydrofolate cyclohydrolase

Specific Activity

Specific Activity on EC 3.5.4.9 - methenyltetrahydrofolate cyclohydrolase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0002
-
cyclohydrolase activity, rat liver, formation of 5-formyltetrahydrofolate
0.09
-
source spleen
0.13
-
source kidney
0.16
-
source spleen
0.18
-
source liver
0.22
-
source liver
0.25
-
source kidney
0.27
-
source liver
0.28
-
0 day-old seedling
0.35
-
source kidney
0.36
-
source kidney
0.37
-
9 day-old seedling
0.4
-
source liver
0.41
-
source kidney
0.42
-
HCOOH/CO as growing substrate
0.434
-
-
0.44
-
3 day-old seedling
0.49
-
growth conditions H2 + CO2
0.63
-
growth conditions glucose + CO2
0.65
-
-
0.72
-
6 day-old sprout
0.82
-
source liver
0.87
-
-
0.94
-
growth conditions methanol + CO2
1.16
-
HCOOH as growing substrate
1.6
mutant K56R
113
recombinant mutant R250A, cyclohydrolase forward reaction
1173
-
forward reaction, recombinant mutant R173A, substrate 5,10-methenyltetrahydrofolate
12.8
-
reverse reaction, recombinant mutant R173K, substrate 10-formyltetrahydrofolate, in presence of 2',5'-ADP
128
mutantS49A
13.5
-
forward reaction, recombinant mutant S197A, substrate 5,10-methenyltetrahydrofolate
133
DC301, wild-type
146
mutants C147A, T148A
15.9
-
reverse reaction, recombinant mutant S197T, substrate 10-formyltetrahydrofolate, in presence of 2',5'-ADP
160
mutant Y52F
161
recombinant wild-type enzyme, cyclohydrolase forward reaction
18
mutant C147Q
2 - 3
pH 8.0, 37°C, extract from Escherichia coli cells carrying the expression vector
2.1
Petroselinum sp.
-
-
2.2
-
-
2.4
-
source leaf
2.7
-
source root
22.4
-
reverse reaction, recombinant wild-type enzyme, substrate 10-formyltetrahydrofolate, in presence of 2',5'-ADP
22.8
-
reverse reaction, recombinant mutant S197R, substrate 10-formyltetrahydrofolate, in presence of 2',5'-ADP
25
mutant S49Q
3.4
-
forward reaction, recombinant mutant S197R, substrate 5,10-methenyltetrahydrofolate
3.7
-
source petiole
32.9
-
purified enzyme
329
-
forward reaction, recombinant mutant R173K, substrate 5,10-methenyltetrahydrofolate
39
mutant Y52S
47
mutant Y52A
470
-
purified enzyme, pH 7.2, 35°C
51
recombinant loop3G mutant, cyclohydrolase forward reaction
6.3
-
cyclohydrolase activity is monitored by following the consumption of 5,10-methylenetetrahydrofolate yielding 10-formyltetrahydrofolate
6.4
-
reverse reaction, recombinant mutant S197A, substrate 10-formyltetrahydrofolate, in absence of 2',5'-ADP
6.7
mutants C147Q, S49Q
61.6
-
forward reaction, recombinant mutant S197D, substrate 5,10-methenyltetrahydrofolate
66
pH 8.0, 37°C, purified enzyme, recombinant
66.7
recombinant loop5G mutant, cyclohydrolase forward reaction
7.2
-
forward reaction, recombinant wild-type enzyme, substrate 5,10-methenyltetrahydrofolate
7.3
-
reverse reaction, recombinant mutant S197R, substrate 10-formyltetrahydrofolate, in absence of 2',5'-ADP
7.6
-
reverse reaction, recombinant mutant S197A, substrate 10-formyltetrahydrofolate, in presence of 2',5'-ADP
76.5
recombinant mutant Y240A, cyclohydrolase forward reaction
8
-
reverse reaction, recombinant mutant R173A, substrate 10-formyltetrahydrofolate, in presence of 2',5'-ADP
9
-
reverse reaction, recombinant mutant R173K, substrate 10-formyltetrahydrofolate, in absence of 2',5'-ADP
9.3
-
reverse reaction, recombinant mutant S197D, substrate 10-formyltetrahydrofolate, in absence of 2',5'-ADP
9.6
-
reverse reaction, recombinant wild-type enzyme, substrate 10-formyltetrahydrofolate, in absence of 2',5'-ADP
97.4
-
recombinant enzyme
additional information