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3.5.4.9: methenyltetrahydrofolate cyclohydrolase

This is an abbreviated version!
For detailed information about methenyltetrahydrofolate cyclohydrolase, go to the full flat file.

Word Map on EC 3.5.4.9

Reaction

5,10-methenyltetrahydrofolate
+
H2O
=
10-formyltetrahydrofolate

Synonyms

5,10-CH+-H4PteGlu cyclohydrolase, 5,10-CH+-THF cyclohydrolase, 5,10-CH=H4 folate synthetase, 5,10-methenyl-H4folate cyclohydrolase, 5,10-methenyl-THF cyclohydrolase, 5,10-methenyltetrahydrofolate cyclohydrolase, 5,10-methenyltetrahydrofolate cyclohydrolase/5,10-methylene tetrahydrofolate dehydrogenase, 5,10-Methenyltetrahydrofolate synthetase, 5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase, ADE3, bifunctional methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase, bifunctional N5,N10-methylene-H4F dehydrogenase/N5,N10-methenyl-H4F cyclohydrolase, CH+-THF cyclohydrolase, CHF4CH, Citrovorum factor cyclodehydrase, cyclohydrolase, dehydrogenasse-cyclohydrolase-synthetase, DHCH, DHCH 1, DHCH 2, DHCH1, FchA, FolD, formyl-methenyl-methylenetetrahydrofolate synthetase (combined), methenyl-H4F cyclohydrolase, methenyl-tetrahydrofolate cyclohydrolase, methenyl-THF cyclohydrolase, methenylH4F cyclohydrolase, methenyltetrahydrofolate cyclohydrolase, methenyltetrahydrofolate dehydrogenase/cyclohydrolase, methylene tetrahydrofolate dehydrogenase/cyclohydrolase, methylene-tetrahydrofolate dehydrogenase/cyclohydrolase, methyleneH4folate cyclohydrolase, methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase, methylenetetrahydrofolate dehydrogenase/cyclohydrolase, methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase, methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase/formyltetrahydrofolate synthetase, MIS1, More, mosquito cyclohydrolase, MTHFD1, MTHFD2, MTHFD2L, MTHFDC, N5,N10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase, N5,N10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase, N5,N10-methylenetetrahydrofolate dehydrogenase/N5,N10-methenyltetrahydrofolate cyclohydrolase, NAD-dependent methylenetetrahydrofolate dehydrogenase-cyclohydrolase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.9 methenyltetrahydrofolate cyclohydrolase

Crystallization

Crystallization on EC 3.5.4.9 - methenyltetrahydrofolate cyclohydrolase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
small platelike crystals belong to body centered orthorhombic space group I222
-
crystal structure of DC301, the cytosolic DC domain of the trifunctional enzyme
crystal structure of the enzyme (MTHFD2) in complex with a substrate-based inhibitor and the enzyme cofactors NAD+ and inorganic phosphate
purified recombinant apo-DHCH, hanging drop vapor diffusion, mixing of 0.002 ml 9 mg/ml protein in 50 mM Tris–HCl, 100 mM NaCl, pH 7.5, with 0.002 ml reservoir solution containing 20% PEG 3350 and 0.2 M ammonium acetate, equilibration against 0.07 ml of reservoir solution at 22°C, 3 days, X-ray diffraction structure determination and analysis at 2.7 A resolution, modelling
hanging drop vapor diffusion method, using 25% (w/v) PEG 3350 and 0.2 M magnesium formate
purified enzyme free and in complex with NADP+, sitting drop vapour diffusion method, mixing of 1.8 mg/ml protein in 10 mM Tris-HCl, 50 mM KCl, and 2 mM DTT, with 18% PEG 4000, 400 mM NaCl, and 100 mM Tris-HCl, pH 8.0 at 22°C, 5 mM NADP+ is added during crystallization, X-ray diffraction structure determination and analysis, molecular replacement
in the presence of NADP+, at 22°C, using polyethylene glycol 4000 as a precipitant, sitting drop vapour diffusion method
-