Literature summary for 3.5.4.9 extracted from

Eadsforth, T.C.; Gardiner, M.; Maluf, F.V.; McElroy, S.; James, D.; Frearson, J.; Gray, D.; Hunter, W.N.
Assessment of Pseudomonas aeruginosa N5,N10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase as a potential antibacterial drug target (2012), PLoS ONE, 7, e35973.

Search for more literature for 3.5.4.9

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 25% (w/v) PEG 3350 and 0.2 M magnesium formate	Pseudomonas aeruginosa

Inhibitors

Inhibitors	Comment	Organism	Structure
2-[(2-chloro-6-fluorobenzyl)sulfanyl]-6-(trifluoromethyl)pyrimidin-4-ol	-	Pseudomonas aeruginosa
5-[(4-chlorophenyl)sulfonyl]-6-ethoxypyrimidine-2,4-diamine	-	Pseudomonas aeruginosa
6-benzyl-5-methyl-7-oxo-4,7-dihydropyrazolo[1,5-a]pyrimidine-3-carbonitrile	-	Pseudomonas aeruginosa
LY354899	i.e. 5,6,7,8-tetrahydro-N5,N10-carbonylfolic acid	Pseudomonas aeruginosa
LY374571	i.e. (2R)-2-[(4-{[(2,5-diamino-6-hydroxypyrimidin-4-yl)carbamoyl]amino}phenyl)formamido]pentanedioic acid	Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.026	-	5,10-methenyltetrahydrofolate	at pH 7.9 and 22°C	Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
30000	-	2 * 30000, SDS-PAGE	Pseudomonas aeruginosa
60000	-	gel filtration	Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
5,10-methenyltetrahydrofolate + H2O	Pseudomonas aeruginosa	-	10-formyltetrahydrofolate	-	r

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	Q9I2U6	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+-charged HisTrap column chromatography and Superdex 200 gel filtration	Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5,10-methenyltetrahydrofolate + H2O	-	Pseudomonas aeruginosa	10-formyltetrahydrofolate	-	r

Subunits

Subunits	Comment	Organism
homodimer	2 * 30000, SDS-PAGE	Pseudomonas aeruginosa

Synonyms

Synonyms	Comment	Organism
FolD	-	Pseudomonas aeruginosa
N5,N10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase	bifunctional enzyme	Pseudomonas aeruginosa

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.00003	-	at pH 7.9 and 22°C	Pseudomonas aeruginosa	LY374571
0.0009	-	at pH 7.9 and 22°C	Pseudomonas aeruginosa	5-[(4-chlorophenyl)sulfonyl]-6-ethoxypyrimidine-2,4-diamine
0.0025	-	at pH 7.9 and 22°C	Pseudomonas aeruginosa	2-[(2-chloro-6-fluorobenzyl)sulfanyl]-6-(trifluoromethyl)pyrimidin-4-ol
0.0038	-	at pH 7.9 and 22°C	Pseudomonas aeruginosa	6-benzyl-5-methyl-7-oxo-4,7-dihydropyrazolo[1,5-a]pyrimidine-3-carbonitrile

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Release 2023.1 (January 2023)