3.5.4.9: methenyltetrahydrofolate cyclohydrolase

This is an abbreviated version!
For detailed information about methenyltetrahydrofolate cyclohydrolase, go to the full flat file.

Word Map on EC 3.5.4.9

Reaction

5,10-methenyltetrahydrofolate
+
H2O
=
10-formyltetrahydrofolate

Synonyms

More, 5,10-Methenyltetrahydrofolate synthetase, Citrovorum factor cyclodehydrase, cyclohydrolase, formyl-methenyl-methylenetetrahydrofolate synthetase (combined), 5,10-methenyl-H4folate cyclohydrolase, 5,10-CH+-H4PteGlu cyclohydrolase, CHF4CH, mosquito cyclohydrolase, methenyl-THF cyclohydrolase, methenylH4F cyclohydrolase, bifunctional methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase, methyleneH4folate cyclohydrolase, methenyltetrahydrofolate dehydrogenase/cyclohydrolase, 5,10-CH=H4 folate synthetase, 5,10-methenyltetrahydrofolate cyclohydrolase, MTHFD1, methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase/formyltetrahydrofolate synthetase, 5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase, MTHFDC, DHCH1, methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase, methylene tetrahydrofolate dehydrogenase/cyclohydrolase, 5,10-methenyltetrahydrofolate cyclohydrolase/5,10-methylene tetrahydrofolate dehydrogenase, dehydrogenasse-cyclohydrolase-synthetase, ADE3, MIS1, MTHFD2, NAD-dependent methylenetetrahydrofolate dehydrogenase-cyclohydrolase, methenyltetrahydrofolate cyclohydrolase, methylene-tetrahydrofolate dehydrogenase/cyclohydrolase, DHCH 1, DHCH 2, methenyl-tetrahydrofolate cyclohydrolase, 5,10-methenyl-THF cyclohydrolase, methylenetetrahydrofolate dehydrogenase/cyclohydrolase, N5,N10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase, N5,N10-methylenetetrahydrofolate dehydrogenase/N5,N10-methenyltetrahydrofolate cyclohydrolase, DHCH, MTHFD2L, FolD, bifunctional N5,N10-methylene-H4F dehydrogenase/N5,N10-methenyl-H4F cyclohydrolase, methenyl-H4F cyclohydrolase, CH+-THF cyclohydrolase, N5,N10-methylenetetrahydrofolate dehydrogenase-cyclohydrolase, methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase, FchA, 5,10-CH+-THF cyclohydrolase

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.4 In cyclic amidines
                3.5.4.9 methenyltetrahydrofolate cyclohydrolase

Storage Stability

Storage Stability on EC 3.5.4.9 - methenyltetrahydrofolate cyclohydrolase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C purified enzyme in 50% glycerol, 95% active after 6 months
-
-20°C, 25% glycerol, 20% cyclohydrolase activity remains after 1 month
-
-20°C, loses 15% activity in 1 day, 50% in 5 days and 70% in 30 days
-
-80°C, 50 mM potassium phosphate, 30% glycerol buffer, stable for over 1 month
-
0°C, relatively unstable, losing 40% activity in 1 day and 75% in 4 days
-
4°C, 25% glycerol, 60% cyclohydrolase activity remains after 1 month
-
5°C, 50 mM phosphate buffer, pH 7.0, stable for at least 1 week
-