3.4.24.64: mitochondrial processing peptidase

This is an abbreviated version!
For detailed information about mitochondrial processing peptidase, go to the full flat file.

Word Map on EC 3.4.24.64

Reaction

Release of N-terminal targetting peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2 =

Synonyms

More, Processing enhancing peptidase, Proteinase, mitochondrial protein precursor-processing, Matrix peptidase, Matrix processing peptidase, Matrix processing proteinase, Mitochondrial protein precursor-processing proteinase, MPP, Mitochondrial chelator-sensitive protease, General mitochondrial processing peptidase, TPP, processing peptidase, Alpha-MPP, Beta-MPP, HA1523, P-52, P-55, PEP, processing enhancing protein, Mas1, Mas2, Plsp1, Atp23, inner membrane peptidase processing enzyme, Imp1, Imp2, mitochondrial processing peptidase, HPP, GPP, TTHA1264, thylakoidal processing peptidase, Plsp2, MPP-alpha, MMP-beta, mitochondrial processing peptidase-alpha protein, PMPCA, mitochondrial processing protease

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.64 mitochondrial processing peptidase

Purification

Purification on EC 3.4.24.64 - mitochondrial processing peptidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 components, a catalytically active MW 55000 and a MW 52000 subunit in a 1:1 ratio
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2 components: MAS1 and MAS2
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alpha- and beta-subunit, isolated as recombinant fusion proteins with maltose-binding protein from Escherichia coli, treated with factor Xa, then separatly purified to homogeneity, active MPP recovered from mixed subunits after denaturation/renaturation procedure
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cytochrome bc1 complex
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from isolated mitochondria
from soluble matrix fraction of isolated mitochondria
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hexahistidine-tagged alpha-MPP and E73Q alpha/beta complex
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hexahistidine-tagged MPP
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histidine-tagged alpha-MPP subunit
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histidine-tagged MPP subunits
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if MAS1 is overproduced in the absence of MAS2 it is insoluble and not suitable for purification, it is therefore purified from the purified holoenzyme
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improved procedure
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native enzyme partially by purification of mitochondria via sucrose gradient centrifugation
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Ni-NTA agarose column chromatography
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recombinant alpha- and beta-subunit separatly and as holoenzyme from Escherichia coli lysate
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recombinant alpha-MPP and native beta-MPP
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recombinant enzyme
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recombinant MPP from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, hydrophobic interaction and anion exchange chromatography, hydroxyapatite chromatography, and gel filtration
soluble matrix enzyme
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wild-type and mutant enzyme
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