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Literature summary for 3.4.24.64 extracted from

  • Saavedra-Alanis, V.M.; Rysavy, P.; Rosenberg, L.E.; Kalousek, F.
    Rat liver mitochondrial processing peptidase. Both alpha- and beta-subunits are required for activity (1994), J. Biol. Chem., 269, 9284-9288.
    View publication on PubMed
Search for more literature for 3.4.24.64

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rattus norvegicus
rat, alpha- and beta-subunit, expressed in Escherichia coli as fusion proteins with maltose-binding protein Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 1 * 50000 (beta-MPP) + 1 * 53000 (alpha-MPP), recombinant enzyme, SDS-PAGE Rattus norvegicus
53000
-
 1 * 50000 (beta-MPP) + 1 * 53000 (alpha-MPP), recombinant enzyme, SDS-PAGE Rattus norvegicus
108000
-
 gel filtration Rattus norvegicus
108000
-
 rat Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
alpha- and beta-subunit, isolated as recombinant fusion proteins with maltose-binding protein from Escherichia coli, treated with factor Xa, then separatly purified to homogeneity, active MPP recovered from mixed subunits after denaturation/renaturation procedure Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
mitochondrial malate dehydrogenase precursor + H2O from rat Rattus norvegicus mitochondrial malate dehydrogenase + mitochondrial malate dehydrogenase transit peptide
-
 ?
Ornithine carbamoyl transferase precursor + H2O EC 2.1.3.3, from rat Rattus norvegicus Ornithine carbamoyl transferase intermediate form
-
 ?
Rieske FES protein precursor + H2O from Neurospora Rattus norvegicus Rieske FES protein intermediate form from Neurospora ?

Subunits

Subunits Comment Organism
dimer 1 * 50000 (beta-MPP) + 1 * 53000 (alpha-MPP), recombinant enzyme, SDS-PAGE Rattus norvegicus