Cloned (Comment) | Organism |
---|---|
sequence comparisons, overview, expression in Escherichia coli strain BL21(DE3) | Thermus thermophilus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant SeMet-labeled enzyme, sitting-drop vapor-diffusion method, 0.001 ml of protein solution containing 22.5 mg/ml protein in 20 mM Tris-HCl (pH 8.0), 150 mM NaCl, and 1 mM DTT, are mixed with 0.001 ml of reservoir solution containing 0.15 M DL-malic acid, pH 7.0, 22% PEG 3350, X-ray diffraction structure determination and analysis at 2.29 A resolution | Thermus thermophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Thermus thermophilus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | a zinc-metallopeptidase, the betaMPP subunit contains the zinc-binding motif, HxxEHx74E, essential for peptidase activity | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45578 | - |
1 * 45578, alphabeta, sequence calculation | Thermus thermophilus |
47000 | - |
gel filtration, recombinant enzyme | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q5SIV0 | - |
- |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | Q5SIV0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant MPP from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, hydrophobic interaction and anion exchange chromatography, hydroxyapatite chromatography, and gel filtration | Thermus thermophilus |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45578, alphabeta, sequence calculation | Thermus thermophilus |
More | TTHA1264 possesses two repetitive domains with a canonical fold for peptidase family M16. The betaMPP subunit contains the zinc-binding motif essential for peptidase activity, while subunit alphaMPP instead possesses a glycine-rich loop, which is proposed to be important for substrate recognition or restriction. Structure analysis, modelling, overview | Thermus thermophilus |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the M16 zinc peptidase family | Thermus thermophilus |
TTHA1264 | - |
Thermus thermophilus |
General Information | Comment | Organism |
---|---|---|
physiological function | MPP is involved in the transport of nuclear-encoded proteins from the cytosol into mitochondria | Thermus thermophilus |