General Stability | Organism |
---|---|
Separation of the loosely associated subunits of MW 48000 and 51000 causes complete loss of activity | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Saccharomyces cerevisiae | |
additional information | PMSF | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | matrix | Saccharomyces cerevisiae | 5739 | - |
soluble | - |
Saccharomyces cerevisiae | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
1 * 48000 (MAS1) + 1 * 51000 (MAS2), Saccharomyces cerevisiae, SDS-PAGE | Saccharomyces cerevisiae |
50000 | - |
gel filtration | Saccharomyces cerevisiae |
51000 | - |
1 * 48000 (MAS1) + 1 * 51000 (MAS2), Saccharomyces cerevisiae, SDS-PAGE | Saccharomyces cerevisiae |
100000 | - |
sucrose density gradient centrifugation | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharomyces cerevisiae | removes amino-terminal matrix-targeting sequences from imported mitochondrial precursor proteins during or after translocation across mitochondrial membranes | ? | - |
? | |
additional information | Saccharomyces cerevisiae D-273-10B | removes amino-terminal matrix-targeting sequences from imported mitochondrial precursor proteins during or after translocation across mitochondrial membranes | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae D-273-10B | - |
- |
- |
Purification (Comment) | Organism |
---|---|
from soluble matrix fraction of isolated mitochondria | Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Release of N-terminal targetting peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2 | cleaves amino-terminal matrix-targeting sequences from imported mitochondrial precursor proteins | Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-70°C, after quick-freezing in liquid nitrogen, at least 1 month | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Cytochrome b2 precursor + H2O | - |
Saccharomyces cerevisiae | Cytochrome b2 intermediate form | - |
? | |
Cytochrome b2 precursor + H2O | - |
Saccharomyces cerevisiae D-273-10B | Cytochrome b2 intermediate form | - |
? | |
Cytochrome c oxidase subunit IV precursor + H2O | - |
Saccharomyces cerevisiae | Cytochrome c oxidase subunit IV | - |
? | |
Cytochrome c oxidase subunit IV precursor + H2O | - |
Saccharomyces cerevisiae D-273-10B | Cytochrome c oxidase subunit IV | - |
? | |
Cytochrome c1 precursor + H2O | membrane-bound enzyme | Saccharomyces cerevisiae | Cytochrome c1 intermediate form | - |
? | |
F1-ATPase beta-subunit precursor + H2O | - |
Saccharomyces cerevisiae | F1-ATPase beta-subunit | - |
? | |
F1-ATPase beta-subunit precursor + H2O | - |
Saccharomyces cerevisiae D-273-10B | F1-ATPase beta-subunit | - |
? | |
Mitochondrial alcohol dehydrogenase precursor + H2O | - |
Saccharomyces cerevisiae | Mitochondrial alcohol dehydrogenase | - |
? | |
additional information | the large subunit alone has no cleavage activity | Saccharomyces cerevisiae | ? | - |
? | |
additional information | removes amino-terminal matrix-targeting sequences from imported mitochondrial precursor proteins during or after translocation across mitochondrial membranes | Saccharomyces cerevisiae | ? | - |
? | |
additional information | the large subunit alone has no cleavage activity | Saccharomyces cerevisiae D-273-10B | ? | - |
? | |
additional information | removes amino-terminal matrix-targeting sequences from imported mitochondrial precursor proteins during or after translocation across mitochondrial membranes | Saccharomyces cerevisiae D-273-10B | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 48000 (MAS1) + 1 * 51000 (MAS2), Saccharomyces cerevisiae, SDS-PAGE | Saccharomyces cerevisiae |
More | Saccharomyces cerevisiae has two loosely associated non-identical subunits that dissociate upon gel filtration | Saccharomyces cerevisiae |
More | the large subunit alone has no cleavage activity | Saccharomyces cerevisiae |