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3.4.23.1: pepsin A

This is an abbreviated version!
For detailed information about pepsin A, go to the full flat file.

Word Map on EC 3.4.23.1

Reaction

Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves Phe1-/-Val, Gln4-/-His, Glu13-/-Ala, Ala14-/-Leu, Leu15-/-Tyr, Tyr16-/-Leu, Gly23-/-Phe, Phe24-/-Phe and Phe25-/-Tyr bonds in the B chain of insulin =

Synonyms

Aspartic proteinase, EC 3.4.4.1, elixir lactate of pepsin, fundus-pepsin, lactated pepsin, lactated pepsin elixir, P I, P-Ia, P-Ib, P-II, P-III, pep/PAG-L, pepsin, pepsin 1, pepsin A, pepsin A1, pepsin A2, pepsin D, pepsin fortior, Pepsin I/II, pepsin R, pepsinogen A, pepsinogen/PAG-Like, pepsins A1, pepsins A2, PG1, PG1-1, PG2, PG2-2, shewasin A, shewasin D

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.23 Aspartic endopeptidases
                3.4.23.1 pepsin A

Purification

Purification on EC 3.4.23.1 - pepsin A

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography of pepsin and pepsinogen using immobilized ligands derived from the specific substrate for this enzyme, optimum conditions of an interaction of the enzyme and its zymogen with immobilized ligands derived from the enzyme substrate: N-acetyl-L-Phe-3,5-diiodo-L-tyrosine
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ammonium sulfate precipitation, DEAE Sepharose column chromatography and Superdex G75 gel filtration
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ammonium sulfate precipitation, DEAE-Sephacel column chromatography, and Sephacryl S-200 gel filtration
ammonium sulfate precipitation, DEAE-Sephacel gel filtration, and Sephacryl S-200 gel filtration
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binding to immobilized inhibitor Val-D-Leu-Pro-Phe-Phe-Val-D-Leu
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Co2+-carged His-Trap HP column chromatography, pepstatin-agarose affinity chromatography, and Mono Q column chromatography
commercial preparation
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DEAE Sephacel column chromatography, Sephadex G-100 gel filtration, and Mono Q column chromatography
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expressed in Escherichia coli
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from rennet
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HisTrap column chromatography and Superdex 200 gel filtration
multiple isoforms
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Ni-NTA column chromatography and Superdex S200 gel filtration
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Sephadex G-100 gel filtration
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stomach extract
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