3.4.23.1: pepsin A

This is an abbreviated version!
For detailed information about pepsin A, go to the full flat file.

Reaction

Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves Phe1-/-Val, Gln4-/-His, Glu13-/-Ala, Ala14-/-Leu, Leu15-/-Tyr, Tyr16-/-Leu, Gly23-/-Phe, Phe24-/-Phe and Phe25-/-Tyr bonds in the B chain of insulin =

Synonyms

Aspartic proteinase, EC 3.4.4.1, elixir lactate of pepsin, fundus-pepsin, lactated pepsin, lactated pepsin elixir, P I, P-Ia, P-Ib, P-II, P-III, pep/PAG-L, pepsin, pepsin 1, pepsin A, pepsin A1, pepsin A2, pepsin D, pepsin fortior, Pepsin I/II, pepsin R, pepsinogen A, pepsinogen/PAG-Like, pepsins A1, pepsins A2, PG1, PG1-1, PG2, PG2-2, shewasin A, shewasin D

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.23 Aspartic endopeptidases

                3.4.23.1 pepsin A

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Results	in table
3	Activating Compound
154	AA Sequence
10	Application
1	CAS Registry Number
11	Cloned(Commentary)
11	Crystallization (Commentary)
2972	Disease/ Diagnostics
1	General Information
3	General Stability
10	IC50 Value
151	Inhibitors
46	kcat/KM [mM/s]
5	Ki Value [mM]
145	KM Value [mM]
1	Localization
3	Metals/Ions
30	Molecular Weight [Da]
11	Natural Substrates/ Products (Substrates)
6	Organic Solvent Stability
124	Organism
23	PDB ID
51	pH Optimum
25	pH Range
24	pH Stability
11	pI Value
8	Posttranslational Modification
34	Protein Variants
40	Purification (Commentary)
1	Reaction
1	Reaction Type
121	Reference
4	Renatured (Commentary)
49	Source Tissue
10	Specific Activity [micromol/min/mg]
4	Storage Stability
235	Substrates and Products (Substrate)
25	Subunits
58	Synonyms
25	Temperature Optimum [°C]
7	Temperature Range [°C]
10	Temperature Stability [°C]
136	Turnover Number [1/s]

KCat KM Value

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KCat KM Value on EC 3.4.23.1 - pepsin A

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kcat/KM VALUE [1/mMs^-1]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

400

((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe-Phe-Ala-Leu-Lys-(2,4-dinitrophenyl)

Shewanella denitrificans

Q12R32

at pH 4.0 and 37°C

755425

190000 - 2400000

Acid-denatured hemoglobin

3 entries

-

29 - 110

AFPLEFEREL

2 entries

1000 - 2800

AFPLEFFREL

2 entries

730 - 2100

AFPLEFIREL

2 entries

70.6 - 180

benzyloxycarbonyl-Gly-Gly-Phe-Phe 3-(4-pyridyl)propyl ester

2 entries

2 - 7.8

benzyloxycarbonyl-Gly-Phe-Phe 3-(4-pyridyl)propyl ester

2 entries

0.7 - 3.7

benzyloxycarbonyl-Phe-Phe 3-(4-pyridyl)propyl ester

2 entries

0.31 - 2.65

benzyloxycarbonyl-Phe-Phe-OEt

4 entries

22.1

bovine serum albumin

Sus scrofa

-

at 43 mM ionic strength, at pH 2.0 and 37°C

753632

-

130 - 3500

hemoglobin

4 entries

-

19.9 - 1306.2

KPAEFFRL

15 entries

2000

Lys-Pro-Ala-Glu-Phe-Phe(NO2)-Ala-Leu

Sus scrofa

-

pH not specified in the publication, temperature not specified in the publication

711237

5.6

Mca-KKPAEFFALK-Dnp

Shewanella amazonensis

A1S3N8

at pH 4.0 and 37°C

717595

93 - 170

POMC (165-174)

2 entries

91 - 1140

Substance P

2 entries

190000

Acid-denatured hemoglobin

Oncorhynchus mykiss

-

isoform P-I, at pH 2.0 and 37°C

753464

-

640000

Acid-denatured hemoglobin

Oncorhynchus mykiss

-

isoform P-II, at pH 2.0 and 37°C

753464

-

2400000

Acid-denatured hemoglobin

Oncorhynchus mykiss

-

isoform P-III, at pH 2.0 and 37°C

753464

-

29

AFPLEFEREL

Homo sapiens

-

in 0.2 M sodium formate buffer, pH 4.0, at 37°C

708952

110

AFPLEFEREL

Sus scrofa

-

in 0.2 M sodium formate buffer, pH 4.0, at 37°C

708952

1000

AFPLEFFREL

Homo sapiens

-

in 0.2 M sodium formate buffer, pH 4.0, at 37°C

708952

2800

AFPLEFFREL

Sus scrofa

-

in 0.2 M sodium formate buffer, pH 4.0, at 37°C

708952

730

AFPLEFIREL

Homo sapiens

-

in 0.2 M sodium formate buffer, pH 4.0, at 37°C

708952

2100

AFPLEFIREL

Sus scrofa

-

in 0.2 M sodium formate buffer, pH 4.0, at 37°C

708952

70.6

benzyloxycarbonyl-Gly-Gly-Phe-Phe 3-(4-pyridyl)propyl ester

Sus scrofa

-

at pH 2.0 and 37°C

753632

180

benzyloxycarbonyl-Gly-Gly-Phe-Phe 3-(4-pyridyl)propyl ester

Sus scrofa

-

at pH 3.5 and 37°C

753632

2

benzyloxycarbonyl-Gly-Phe-Phe 3-(4-pyridyl)propyl ester

Sus scrofa

-

at pH 2.0 and 37°C

753632

7.8

benzyloxycarbonyl-Gly-Phe-Phe 3-(4-pyridyl)propyl ester

Sus scrofa

-

at pH 3.5 and 37°C

753632

0.7

benzyloxycarbonyl-Phe-Phe 3-(4-pyridyl)propyl ester

Sus scrofa

-

at pH 2.0 and 37°C

753632

3.7

benzyloxycarbonyl-Phe-Phe 3-(4-pyridyl)propyl ester

Sus scrofa

-

at pH 3.0 and 37°C

753632

0.31

benzyloxycarbonyl-Phe-Phe-OEt

Sus scrofa

-

at pH 2.0 and 37°C

753632

0.35

benzyloxycarbonyl-Phe-Phe-OEt

Sus scrofa

-

at pH 1.5 and 37°C

753632

0.39

benzyloxycarbonyl-Phe-Phe-OEt

Sus scrofa

-

at pH 1.0 and 37°C

753632

2.65

benzyloxycarbonyl-Phe-Phe-OEt

Sus scrofa

-

at 43 mM ionic strength, at pH 5.5 and 37°C

753632

130

hemoglobin

Lateolabrax japonicus

E2IKQ9

isoform pepsin 1, in 250 mM sodium acetate buffer (pH 3.0), at 37°C

717514

-

190

hemoglobin

Monopterus albus

-

isoform pepsin 1, in 250 mM sodium acetate buffer (pH 3.0), at 37°C

717613

-

1600

hemoglobin

Micropterus salmoides

-

isoform PG1-1, at pH 3.0 and 37°C

753325

-

3500

hemoglobin

Micropterus salmoides

-

isoform PG2-2, at pH 3.0 and 37°C

753325

-

19.9

KPAEFFRL

Micropterus salmoides

A0A125SQG8, A0A125SQG9

pepsin A1, at pH 4.0 and 10°C

753326

36.8

KPAEFFRL

Micropterus salmoides

A0A125SQG8, A0A125SQG9

pepsin A1, at pH 4.0 and 20°C

753326

70.2

KPAEFFRL

Sus scrofa

-

at pH 4.0 and 10°C

753326

164.6

KPAEFFRL

Micropterus salmoides

A0A125SQG8, A0A125SQG9

pepsin A1, at pH 4.0 and 30°C

753326

186.2

KPAEFFRL

Sus scrofa

-

at pH 4.0 and 20°C

753326

218.5

KPAEFFRL

Micropterus salmoides

A0A125SQG8, A0A125SQG9

pepsin A2, at pH 4.0 and 10°C

753326

251.3

KPAEFFRL

Micropterus salmoides

A0A125SQG8, A0A125SQG9

pepsin A1, at pH 4.0 and 37°C

753326

283

KPAEFFRL

Micropterus salmoides

A0A125SQG8, A0A125SQG9

pepsin A2, at pH 4.0 and 20°C

753326

286.3

KPAEFFRL

Micropterus salmoides

A0A125SQG8, A0A125SQG9

pepsin A1, at pH 4.0 and 45°C

753326

475.4

KPAEFFRL

Sus scrofa

-

at pH 4.0 and 30°C

753326

598.9

KPAEFFRL

Sus scrofa

-

at pH 4.0 and 37°C

753326

604.8

KPAEFFRL

Micropterus salmoides

A0A125SQG8, A0A125SQG9

pepsin A2, at pH 4.0 and 30°C

753326

959.9

KPAEFFRL

Micropterus salmoides

A0A125SQG8, A0A125SQG9

pepsin A2, at pH 4.0 and 45°C

753326

999.6

KPAEFFRL

Micropterus salmoides

A0A125SQG8, A0A125SQG9

pepsin A2, at pH 4.0 and 37°C

753326

1306.2

KPAEFFRL

Sus scrofa

-

at pH 4.0 and 45°C

753326

93

POMC (165-174)

Homo sapiens

-

in 0.2 M sodium formate buffer, pH 4.0, at 37°C

708952

170

POMC (165-174)

Sus scrofa

-

in 0.2 M sodium formate buffer, pH 4.0, at 37°C

708952

91

Substance P

Micropterus salmoides

-

isoform PG1-1, at pH 3.0 and 37°C

753325

1140

Substance P

Micropterus salmoides

-

isoform PG2-2, at pH 3.0 and 37°C

753325