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Literature summary for 3.4.23.1 extracted from

  • Shintani, T.; NOmura, K.; Ichishima, E.
    Engineering of porcine pepsin. Alteration of S1 substrate specificity of pepsin to those of fungal aspartic proteinases by site-directed mutagenesis (1997), J. Biol. Chem., 272, 18855-18861.
    View publication on PubMed
Search for more literature for 3.4.23.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sus scrofa

Protein Variants

Protein Variants Comment Organism
G78(S)S79 differences in substrate specificity and catalytic activity Sus scrofa
T77D differences in substrate specificity and catalytic activity Sus scrofa
T77D/G78(S)S79 double mutant, differences in substrate specificity and catalytic activity Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 Pro-Thr-Glu-Phe-Phe(4-NO2)-Arg-Leu wild-type Sus scrofa
0.06
-
 Pro-Thr-Glu-Phe-Phe(4-NO2)-Arg-Leu T77D/G78(S)S79 double mutant Sus scrofa
0.15
-
 Pro-Thr-Glu-Lys-Phe(4-NO2)-Arg-Leu-NH2 T77D/G78(S)S79 double mutant Sus scrofa
0.16
-
 acetyl-Ala-Ala-Lys-Phe(4-NO2)-Ala-Ala-NH2 T77D/G78(S)S79 double mutant Sus scrofa
0.17
-
 Pro-Thr-Glu-Phe-Phe(4-NO2)-Arg-Leu G78(S)S79 mutant Sus scrofa
0.18
-
 Pro-Thr-Glu-Lys-Phe(4-NO2)-Arg-Leu-NH2 T77D mutant Sus scrofa
0.2
-
 Pro-Thr-Glu-Phe-Phe(4-NO2)-Arg-Leu T77D mutant Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
expressed in Escherichia coli Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-Ala-Ala-Lys-Phe(4-NO2)-Ala-Ala-NH2 + H2O
-
 Sus scrofa ?
-
 ?
Hemoglobin + H2O
-
 Sus scrofa ?
-
 ?
Pro-Thr-Glu-Lys-Phe(4-NO2)-Arg-Leu-NH2 + H2O
-
 Sus scrofa ?
-
 ?
Pro-Thr-Glu-Phe-Phe(4-NO2)-Arg-Leu + H2O
-
 Sus scrofa ?
-
 ?