Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Trematomus bernacchii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
pepstatin A | requires an inhibitor/enzyme ratio of 1:10 to reach about 50% inhibition; requires an inhibitor/enzyme ratio of 1:10 to reach about 60% inhibition | Trematomus bernacchii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
Pro-Thr-Glu-Phe-(p-nitro-Phe)-Arg-Leu-OH | 37°C, acidic pH | Trematomus bernacchii | |
0.074 | - |
Pro-Thr-Glu-Phe-(p-nitro-Phe)-Arg-Leu-OH | 37°C, acidic pH | Trematomus bernacchii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34250 | - |
ESI mass spectrometry | Trematomus bernacchii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trematomus bernacchii | Q6HA04 | precursor | - |
Trematomus bernacchii | Q6HA05 | precursor | - |
Purification (Comment) | Organism |
---|---|
- |
Trematomus bernacchii |
Renatured (Comment) | Organism |
---|---|
renaturation of the supernatant containing pepsinogen A1 is achieved by a 200fold dilution of the denaturant in 100 mm Tris/HCl buffer (pH 8.0) | Trematomus bernacchii |
renaturation of the supernatant containing pepsinogen A2 is achieved by a 200fold dilution of the denaturant in 100 mm Tris/HCl buffer (pH 8.0) | Trematomus bernacchii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no digestion of Phe-Gly-His-Phe-(p-nitro-Phe)-Ala-Phe-OMe | Trematomus bernacchii | ? | - |
? | |
Pro-Thr-Glu-Phe-(p-nitro-Phe)-Arg-Leu-OH + H2O | - |
Trematomus bernacchii | Pro-Thr-Glu-Phe + (p-nitro-Phe)-Arg-Leu-OH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
pepsins A1 | - |
Trematomus bernacchii |
pepsins A2 | - |
Trematomus bernacchii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Trematomus bernacchii |
50 | - |
- |
Trematomus bernacchii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | 25°C: about 70% of maximal activity, 50°C: about 90% of maximal activity | Trematomus bernacchii |
25 | 60 | 25°C: about 50% of maximal activity, 60°C: about 10% of maximal activity | Trematomus bernacchii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 5.3, half-life: 270 min | Trematomus bernacchii |
50 | - |
pH 5.3, half-life: 72 min | Trematomus bernacchii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.54 | - |
Pro-Thr-Glu-Phe-(p-nitro-Phe)-Arg-Leu-OH | 37°C, acidic pH | Trematomus bernacchii | |
3.32 | - |
Pro-Thr-Glu-Phe-(p-nitro-Phe)-Arg-Leu-OH | 37°C, acidic pH | Trematomus bernacchii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2 | - |
- |
Trematomus bernacchii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
1 | 3.5 | pH 1.0: about 85% of maximal activity, pH 3.5: about 40% of maximal activity | Trematomus bernacchii |
1 | 4 | pH 1.0: about 70% of maximal activity, pH 4.0: about 50% of maximal activity | Trematomus bernacchii |