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Literature summary for 3.4.23.1 extracted from

  • Brier, S.; Maria, G.; Carginale, V.; Capasso, A.; Wu, Y.; Taylor, R.M.; Borotto, N.B.; Capasso, C.; Engen, J.R.
    Purification and characterization of pepsins A1 and A2 from the Antarctic rock cod Trematomus bernacchii (2007), FEBS J., 274, 6152-6166.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.23.1

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Trematomus bernacchii

Inhibitors

Inhibitors Comment Organism Structure
pepstatin A requires an inhibitor/enzyme ratio of 1:10 to reach about 50% inhibition; requires an inhibitor/enzyme ratio of 1:10 to reach about 60% inhibition Trematomus bernacchii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
 Pro-Thr-Glu-Phe-(p-nitro-Phe)-Arg-Leu-OH 37°C, acidic pH Trematomus bernacchii
0.074
-
 Pro-Thr-Glu-Phe-(p-nitro-Phe)-Arg-Leu-OH 37°C, acidic pH Trematomus bernacchii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34250
-
 ESI mass spectrometry Trematomus bernacchii

Organism

Organism UniProt Comment Textmining
Trematomus bernacchii Q6HA04 precursor
-
Trematomus bernacchii Q6HA05 precursor
-

Purification (Commentary)

Purification (Comment) Organism
-
 Trematomus bernacchii

Renatured (Commentary)

Renatured (Comment) Organism
renaturation of the supernatant containing pepsinogen A1 is achieved by a 200fold dilution of the denaturant in 100 mm Tris/HCl buffer (pH 8.0) Trematomus bernacchii
renaturation of the supernatant containing pepsinogen A2 is achieved by a 200fold dilution of the denaturant in 100 mm Tris/HCl buffer (pH 8.0) Trematomus bernacchii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no digestion of Phe-Gly-His-Phe-(p-nitro-Phe)-Ala-Phe-OMe Trematomus bernacchii ?
-
 ?
Pro-Thr-Glu-Phe-(p-nitro-Phe)-Arg-Leu-OH + H2O
-
 Trematomus bernacchii Pro-Thr-Glu-Phe + (p-nitro-Phe)-Arg-Leu-OH
-
 ?

Synonyms

Synonyms Comment Organism
pepsins A1
-
 Trematomus bernacchii
pepsins A2
-
 Trematomus bernacchii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Trematomus bernacchii
50
-
-
 Trematomus bernacchii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50 25°C: about 70% of maximal activity, 50°C: about 90% of maximal activity Trematomus bernacchii
25 60 25°C: about 50% of maximal activity, 60°C: about 10% of maximal activity Trematomus bernacchii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 pH 5.3, half-life: 270 min Trematomus bernacchii
50
-
 pH 5.3, half-life: 72 min Trematomus bernacchii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.54
-
 Pro-Thr-Glu-Phe-(p-nitro-Phe)-Arg-Leu-OH 37°C, acidic pH Trematomus bernacchii
3.32
-
 Pro-Thr-Glu-Phe-(p-nitro-Phe)-Arg-Leu-OH 37°C, acidic pH Trematomus bernacchii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2
-
-
 Trematomus bernacchii

pH Range

pH Minimum pH Maximum Comment Organism
1 3.5 pH 1.0: about 85% of maximal activity, pH 3.5: about 40% of maximal activity Trematomus bernacchii
1 4 pH 1.0: about 70% of maximal activity, pH 4.0: about 50% of maximal activity Trematomus bernacchii