Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.4.23.1: pepsin A

This is an abbreviated version!
For detailed information about pepsin A, go to the full flat file.

Word Map on EC 3.4.23.1

Reaction

Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves Phe1-/-Val, Gln4-/-His, Glu13-/-Ala, Ala14-/-Leu, Leu15-/-Tyr, Tyr16-/-Leu, Gly23-/-Phe, Phe24-/-Phe and Phe25-/-Tyr bonds in the B chain of insulin =

Synonyms

Aspartic proteinase, EC 3.4.4.1, elixir lactate of pepsin, fundus-pepsin, lactated pepsin, lactated pepsin elixir, P I, P-Ia, P-Ib, P-II, P-III, pep/PAG-L, pepsin, pepsin 1, pepsin A, pepsin A1, pepsin A2, pepsin D, pepsin fortior, Pepsin I/II, pepsin R, pepsinogen A, pepsinogen/PAG-Like, pepsins A1, pepsins A2, PG1, PG1-1, PG2, PG2-2, shewasin A, shewasin D

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.23 Aspartic endopeptidases
                3.4.23.1 pepsin A

KM Value

KM Value on EC 3.4.23.1 - pepsin A

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00867
((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe-Phe-Ala-Leu-Lys-(2,4-dinitrophenyl)
at pH 4.0 and 37°C
0.16
acetyl-Ala-Ala-Lys-Phe(4-NO2)-Ala-Ala-NH2
-
T77D/G78(S)S79 double mutant
0.0000079 - 0.000028
Acid-denatured hemoglobin
-
0.3 - 0.4
AFPLEFEREL
0.084 - 0.17
AFPLEFFREL
0.079 - 0.098
AFPLEFIREL
0.4 - 0.8
benzyloxycarbonyl-Gly-Gly-Phe-Phe 3-(4-pyridyl)propyl ester
0.4 - 1.1
benzyloxycarbonyl-Gly-Phe-Phe 3-(4-pyridyl)propyl ester
0.49
benzyloxycarbonyl-His-Phe-Phe-NH2
-
-
0.18
benzyloxycarbonyl-His-Phe-Phe-O-ethyl ester
-
-
0.37
benzyloxycarbonyl-His-Phe-Phe-O-methyl ester
-
-
0.25
benzyloxycarbonyl-His-Trp-Phe-O-methyl ester
-
-
0.68
benzyloxycarbonyl-His-Tyr-Phe-O-methyl ester
-
-
0.2 - 0.7
benzyloxycarbonyl-Phe-Phe 3-(4-pyridyl)propyl ester
0.04 - 0.37
benzyloxycarbonyl-Phe-Phe-OEt
0.2
bovine serum albumin
-
at 43 mM ionic strength, at pH 2.0 and 37°C
-
0.76 - 1.05
casein
0.02 - 0.12
hemoglobin
-
0.034 - 0.083
KPAEFF(NO2)AL
0.04 - 0.3
KPAEFFRL
0.011 - 0.035
KPIEF(NO2)RL
0.03 - 0.16
KPIKF(NO2)RL
0.0051 - 0.0093
KPILF(NO2)RL
0.015 - 0.05
KPIQF(NO2)RL
0.013 - 0.074
KPPEF(NO2)RL
0.048 - 0.075
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu
0.019
Leu-Ser-Phe(NO2)-Nle-Ala-Leu
-
-
0.011 - 0.044
Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu
0.015 - 0.041
LSF(NO2)-Nle-AL
0.034
Lys-Pro-Ala-Glu-Phe-Phe(4NO2)-Ala-Leu
-
-
0.0433
Lys-Pro-Ala-Glu-Phe-Phe(NO2)-Ala-Leu
-
pH 5.3, 25°C
0.043 - 0.098
Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu
0.0054
Mca-KKPAEFFALK-Dnp
at pH 4.0 and 37°C
0.05
N,N-dimethylcasein
-
-
-
0.046
N,N-dimethylhemoglobin
-
-
-
0.022 - 0.029
POMC (165-174)
0.15 - 0.18
Pro-Thr-Glu-Lys-Phe(4-NO2)-Arg-Leu-NH2
0.025 - 0.074
Pro-Thr-Glu-Phe-(p-nitro-Phe)-Arg-Leu-OH
0.04 - 0.2
Pro-Thr-Glu-Phe-Phe(4-NO2)-Arg-Leu
0.0344 - 0.698
Substance P
additional information
additional information
-