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Literature summary for 3.4.23.1 extracted from

  • Wald, M.; Rehbein, H.; Beermann, C.; Bussmann, B.; Schwarz, K.
    Purification and characterization of pepsinogen and pepsin from the stomach of rainbow trout (Oncorhynchus mykiss) (2016), Eur. Food Res. Technol., 242, 1925-1935 .
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by EDTA, E-64, leupeptin, 4-(2-aminoethyl)benzenesulfonyl fluoride or aprotinin Oncorhynchus mykiss
pepstatin A
-
Oncorhynchus mykiss

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000079
-
Acid-denatured hemoglobin isoform P-III, at pH 2.0 and 37°C Oncorhynchus mykiss
0.000013
-
Acid-denatured hemoglobin isoform P-II, at pH 2.0 and 37°C Oncorhynchus mykiss
0.000028
-
Acid-denatured hemoglobin isoform P-I, at pH 2.0 and 37°C Oncorhynchus mykiss

Organism

Organism UniProt Comment Textmining
Oncorhynchus mykiss
-
rainbow trout
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE Sepharose column chromatography and Superdex G75 gel filtration Oncorhynchus mykiss

Source Tissue

Source Tissue Comment Organism Textmining
stomach
-
Oncorhynchus mykiss
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Acid-denatured hemoglobin + H2O
-
Oncorhynchus mykiss ?
-
?

Subunits

Subunits Comment Organism
? x * 35000, isoform P-II, SDS-PAGE Oncorhynchus mykiss
? x * 37000, isoform P-Ia, SDS-PAGE Oncorhynchus mykiss
? x * 37000, isoform P-Ib, SDS-PAGE Oncorhynchus mykiss
? x * 37000, isoform P-III, SDS-PAGE Oncorhynchus mykiss

Synonyms

Synonyms Comment Organism
P-Ia isoform Oncorhynchus mykiss
P-Ib isoform Oncorhynchus mykiss
P-II isoform Oncorhynchus mykiss
P-III isoform Oncorhynchus mykiss

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
isoforms P-II and P-III Oncorhynchus mykiss
40
-
isoform P-I Oncorhynchus mykiss

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.43
-
Acid-denatured hemoglobin isoform P-I, at pH 2.0 and 37°C Oncorhynchus mykiss
8.37
-
Acid-denatured hemoglobin isoform P-II, at pH 2.0 and 37°C Oncorhynchus mykiss
19.1
-
Acid-denatured hemoglobin isoform P-III, at pH 2.0 and 37°C Oncorhynchus mykiss

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5
-
isoforms P-II and P-III Oncorhynchus mykiss
3
-
isoform P-I Oncorhynchus mykiss

pH Range

pH Minimum pH Maximum Comment Organism
1 6 trout pepsins exhibit the highest enzyme activity at pH 3.0 (isoform P-I) and 2.5 (isoforms P-II and P-III); P-I shows a broad optimum in the pH range from 1.5 to 4.0, while the optima for P-II and III are restricted to the pH range from 2.0 to 3.0.at pH 4.0. Isoform P-I retains 94% activity, while isoforms P-II 72 and P-III have 65% activity. All three isoforms show residual activity of about 20% at pH 6.0 Oncorhynchus mykiss

pI Value

Organism Comment pI Value Maximum pI Value
Oncorhynchus mykiss isoform P-IIIa, isoelectric focusing
-
3.7
Oncorhynchus mykiss isoform P-IIIb, isoelectric focusing
-
3.8
Oncorhynchus mykiss isoform P-Ia, isoelectric focusing
-
4
Oncorhynchus mykiss isoform P-Ib, isoelectric focusing
-
4
Oncorhynchus mykiss isoform P-IIIc, isoelectric focusing
-
4
Oncorhynchus mykiss isoform P-IIId, isoelectric focusing
-
4.2
Oncorhynchus mykiss isoform P-II, isoelectric focusing
-
5.9

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
190000
-
Acid-denatured hemoglobin isoform P-I, at pH 2.0 and 37°C Oncorhynchus mykiss
640000
-
Acid-denatured hemoglobin isoform P-II, at pH 2.0 and 37°C Oncorhynchus mykiss
2400000
-
Acid-denatured hemoglobin isoform P-III, at pH 2.0 and 37°C Oncorhynchus mykiss