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3.51
((7-methoxycoumarin-4-yl)acetyl)-Lys-Lys-Pro-Ala-Glu-Phe-Phe-Ala-Leu-Lys-(2,4-dinitrophenyl)
at pH 4.0 and 37°C
5.43 - 19.1
Acid-denatured hemoglobin
-
56.5 - 71.8
benzyloxycarbonyl-Gly-Gly-Phe-Phe 3-(4-pyridyl)propyl ester
2.2 - 3.1
benzyloxycarbonyl-Gly-Phe-Phe 3-(4-pyridyl)propyl ester
0.21
benzyloxycarbonyl-His-Phe-Phe-NH2
-
-
0.31
benzyloxycarbonyl-His-Phe-Phe-O-ethyl ester
-
-
0.15
benzyloxycarbonyl-His-Phe-Phe-O-methyl ester
-
-
0.013
benzyloxycarbonyl-His-Trp-Phe-O-methyl ester
-
-
0.013
benzyloxycarbonyl-His-Tyr-Phe-O-methyl ester
-
-
0.49 - 0.74
benzyloxycarbonyl-Phe-Phe 3-(4-pyridyl)propyl ester
0.08 - 0.13
benzyloxycarbonyl-Phe-Phe-OEt
4.44
bovine serum albumin
-
at 43 mM ionic strength, at pH 2.0 and 37°C
-
16 - 162
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu
1.2 - 88.7
Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu
56
Lys-Pro-Ala-Glu-Phe-Phe(NO2)-Ala-Leu
-
pH 5.3, 25°C
2.5 - 181
Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu
0.03
Mca-KKPAEFFALK-Dnp
at pH 4.0 and 37°C
7
N,N-dimethylcasein
-
-
-
18.3
N,N-dimethylhemoglobin
-
-
-
0.54 - 3.32
Pro-Thr-Glu-Phe-(p-nitro-Phe)-Arg-Leu-OH
additional information
additional information
-
5.43
Acid-denatured hemoglobin
-
isoform P-I, at pH 2.0 and 37°C
-
8.37
Acid-denatured hemoglobin
-
isoform P-II, at pH 2.0 and 37°C
-
19.1
Acid-denatured hemoglobin
-
isoform P-III, at pH 2.0 and 37°C
-
8.9
AFPLEFEREL
-
in 0.2 M sodium formate buffer, pH 4.0, at 37°C
45
AFPLEFEREL
-
in 0.2 M sodium formate buffer, pH 4.0, at 37°C
85
AFPLEFFREL
-
in 0.2 M sodium formate buffer, pH 4.0, at 37°C
472
AFPLEFFREL
-
in 0.2 M sodium formate buffer, pH 4.0, at 37°C
56
AFPLEFIREL
-
in 0.2 M sodium formate buffer, pH 4.0, at 37°C
203
AFPLEFIREL
-
in 0.2 M sodium formate buffer, pH 4.0, at 37°C
56.5
benzyloxycarbonyl-Gly-Gly-Phe-Phe 3-(4-pyridyl)propyl ester
-
at pH 2.0 and 37°C
71.8
benzyloxycarbonyl-Gly-Gly-Phe-Phe 3-(4-pyridyl)propyl ester
-
at pH 3.5 and 37°C
2.2
benzyloxycarbonyl-Gly-Phe-Phe 3-(4-pyridyl)propyl ester
-
at pH 2.0 and 37°C
3.1
benzyloxycarbonyl-Gly-Phe-Phe 3-(4-pyridyl)propyl ester
-
at pH 3.5 and 37°C
0.49
benzyloxycarbonyl-Phe-Phe 3-(4-pyridyl)propyl ester
-
at pH 2.0 and 37°C
0.74
benzyloxycarbonyl-Phe-Phe 3-(4-pyridyl)propyl ester
-
at pH 3.0 and 37°C
0.08
benzyloxycarbonyl-Phe-Phe-OEt
-
at 43 mM ionic strength, at pH 5.5 and 37°C
0.1
benzyloxycarbonyl-Phe-Phe-OEt
-
at pH 1.0 and 37°C
0.11
benzyloxycarbonyl-Phe-Phe-OEt
-
at pH 2.0 and 37°C
0.13
benzyloxycarbonyl-Phe-Phe-OEt
-
at pH 1.5 and 37°C
7.34
hemoglobin
isoform pepsin 1, in 250 mM sodium acetate buffer (pH 3.0), at 37°C
-
23.2
hemoglobin
-
isoform pepsin 1, in 250 mM sodium acetate buffer (pH 3.0), at 37°C
-
50
hemoglobin
-
pH 3.0, 37°C
-
52.7
hemoglobin
-
isoform PG1-1, at pH 3.0 and 37°C
-
62.9
hemoglobin
-
isoform PG2-2, at pH 3.0 and 37°C
-
5
KPAEFF(NO2)AL
-
mutant enzyme D32E/D215E
6
KPAEFF(NO2)AL
-
mutant enzyme D32E
25
KPAEFF(NO2)AL
-
mutant enzyme D215E
96
KPAEFF(NO2)AL
-
wild-type enzyme
4.69
KPAEFFRL
pepsin A1, at pH 4.0 and 10°C
4.92
KPAEFFRL
pepsin A1, at pH 4.0 and 20°C
8.19
KPAEFFRL
pepsin A1, at pH 4.0 and 30°C
9.73
KPAEFFRL
-
at pH 4.0 and 10°C
12.4
KPAEFFRL
-
at pH 4.0 and 20°C
18.6
KPAEFFRL
pepsin A1, at pH 4.0 and 37°C
18.7
KPAEFFRL
pepsin A2, at pH 4.0 and 10°C
21.9
KPAEFFRL
-
at pH 4.0 and 30°C
28.9
KPAEFFRL
pepsin A2, at pH 4.0 and 20°C
33.3
KPAEFFRL
-
at pH 4.0 and 37°C
36.5
KPAEFFRL
pepsin A1, at pH 4.0 and 45°C
74.7
KPAEFFRL
pepsin A2, at pH 4.0 and 30°C
75.9
KPAEFFRL
-
at pH 4.0 and 45°C
139.2
KPAEFFRL
pepsin A2, at pH 4.0 and 37°C
215.5
KPAEFFRL
pepsin A2, at pH 4.0 and 45°C
0.24
KPIEF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme F111T/L112F/E287M
41.5
KPIEF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme T222V
52.3
KPIEF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme F111T/L112F/T222V/E287M
56.8
KPIEF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme T222V/E287M
60.7
KPIEF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme E287M
68.9
KPIEF(NO2)RL
-
pH 3.5, 25°C, wild-type enzyme
72.5
KPIEF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme F111T/L112F
97.8
KPIEF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme F111T/L112F/E287M
0.63
KPIKF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme F111T/L112F/E287M
32.3
KPIKF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme F111T/L112F/T222V/E287M
38.8
KPIKF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme F111T/L112F
38.9
KPIKF(NO2)RL
-
pH 3.5, 25°C, wild-type enzyme
40.5
KPIKF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme T222V
41.4
KPIKF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme F111T/L112F/E287M
44.2
KPIKF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme E287M
55.5
KPIKF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme T222V/E287M
0.8
KPILF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme T222V/E287M
8
KPILF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme F111T/L112F/E287M
14
KPILF(NO2)RL
-
pH 3.5, 25°C, wild-type enzyme
22.1
KPILF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme T222V
34.4
KPILF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme E287M
35.6
KPILF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme T222V/E287M
36.3
KPILF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme F111T/L112F/T222V/E287M
53.1
KPILF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme F111T/L112F
60.4
KPILF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme F111T/L112F/E287M
0.1
KPIQF(NO2)RL
-
pH 3.5, 25°C, wild-type enzyme
0.19
KPIQF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme T222V
0.24
KPIQF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme F111T/L112F/E287M
0.53
KPIQF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme F111T/L112F
2 - 3.7
KPIQF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme E287M
2.8
KPIQF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme T222V/E287M
47.9
KPIQF(NO2)RL
-
pH 3.5, 25°C, wild-type enzyme
49.7
KPIQF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme T222V
68.9
KPIQF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme F111T/L112F/T222V/E287M
77.4
KPIQF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme T222V/E287M
82.2
KPIQF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme F111T/L112F
85.2
KPIQF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme E287M
97.7
KPIQF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme F111T/L112F/E287M
0.21
KPPEF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme F111T/L112F/E287M
7
KPPEF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme F111T/L112F/T222V/E287M
47.3
KPPEF(NO2)RL
-
pH 3.5, 25°C, wild-type enzyme
58.9
KPPEF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme F111T/L112F/T222V/E287M
62.3
KPPEF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme E287M
62.3
KPPEF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme F111T/L112F
70
KPPEF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme T222V/E287M
71.4
KPPEF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme T222V
79.8
KPPEF(NO2)RL
-
pH 3.5, 25°C, mutant enzyme F111T/L112F/E287M
16
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu
-
unglycosylated mutant S110N/L112T, pH 2.0
53
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu
-
glycosylated mutant T77N, pH 2.0
54
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu
-
glycosylated mutant S110N/L112T, pH 2.0
68.7
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu
-
glycosylated mutant S281N, pH 2.0
70.6
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu
-
glycosylated mutant E244N/V246T, pH 2.0
121
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu
-
unglycosylated mutant E244N/V246T, pH 2.0
127
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu
-
unglycosylated mutant S281N, pH 2.0
150.3
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu
-
unglycosylated mutant S110N/L112T, pH 2.0
154
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu
-
unglycosylated mutant T77N, pH 2.0
162
L-Lys-L-Pro-L-Ala-L-Glu-L-Phe-L-Phe(NO2)-L-Ala-L-Leu
-
wild-type, pH 2.0
1.2
Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu
-
pH 3.95, 37°C, mutant enzyme G76V
2.9
Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu
-
pH 3.95, 37°C, mutant enzyme G76S
23.8
Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu
-
pH 3.95, 37°C, wild-type enzyme
43.4
Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu
-
pH 3.95, 37°C, mutant enzyme G76A
88.7
Leu-Ser-Phe(p-NO2)-Nle-Ala-Leu
-
pH 3.95, 37°C, wild-type enzyme
2.5
Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu
-
pH 2.1, 37°C, mutant enzyme G76S
11.1
Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu
-
pH 2.1, 37°C, mutant enzyme G76V
47
Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu
-
pH 2.1, 37°C, mutant enzyme G76A
180.5
Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu
-
pH 2.1, 37°C, wild-type enzyme
181
Lys-Pro-Ala-Glu-Phe-Phe(p-NO2)-Ala-Leu
-
pH 2.1, 37°C, wild-type enzyme
2.7
POMC (165-174)
-
in 0.2 M sodium formate buffer, pH 4.0, at 37°C
3.7
POMC (165-174)
-
in 0.2 M sodium formate buffer, pH 4.0, at 37°C
0.54
Pro-Thr-Glu-Phe-(p-nitro-Phe)-Arg-Leu-OH
37°C, acidic pH
3.32
Pro-Thr-Glu-Phe-(p-nitro-Phe)-Arg-Leu-OH
37°C, acidic pH
38.8
Substance P
-
isoform PG2-2, at pH 3.0 and 37°C
61
Substance P
-
isoform PG1-1, at pH 3.0 and 37°C
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
salmon
-
-
-
additional information
additional information
shark
-
-
-
additional information
additional information
-
-
-
additional information
additional information
dogfish
-
-
-
additional information
additional information
-
pH-dependence of turnover number of N-trifluoroacetyl aromatic amino acids
-
additional information
additional information
-
turnover numbers for loop mutant enzyme S238-M234 and Y274-T283
-
3.4.23.1 pepsin A
results (
results (
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