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Literature summary for 3.4.23.1 extracted from

  • Cao, M.J.; Chen, W.Q.; Du, C.H.; Yoshida, A.; Lan, W.G.; Liu, G.M.; Su, W.J.
    Pepsinogens and pepsins from Japanese seabass (Lateolabrax japonicus) (2011), Comp. Biochem. Physiol. B, 158, 259-265.
    View publication on PubMed
Search for more literature for 3.4.23.1

Activating Compound

Activating Compound Comment Organism Structure
additional information the pepsinogen isoform converts into the active form pepsin under pH 2.0 Lateolabrax japonicus

Inhibitors

Inhibitors Comment Organism Structure
pepstatin A complete inhibition at a molar ratio of 1:20 (pepstatin A:pepsin) Lateolabrax japonicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.055
-
 hemoglobin isoform pepsin 1, in 250 mM sodium acetate buffer (pH 3.0), at 37°C Lateolabrax japonicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 x * 30000, pepsin isoform P1, SDS-PAGE Lateolabrax japonicus
35000
-
 x * 35000, pepsinogen isoform PG1, SDS-PAGE Lateolabrax japonicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Lateolabrax japonicus the cleavage site of isoform PG1 to produce pepsin is clearly at the 41-42 bond of Phe-Ala ?
-
 ?

Organism

Organism UniProt Comment Textmining
Lateolabrax japonicus E2IKQ9 pepsinogen isoform A1; Japanese seabass
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sephacel column chromatography, and Sephacryl S-200 gel filtration Lateolabrax japonicus

Source Tissue

Source Tissue Comment Organism Textmining
stomach
-
 Lateolabrax japonicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 the specific activity of the crude extract and 7.9fold purified enzyme are 4.0 and 31.5 units/mg. One unit of pepsin activity is defined as an increase of absorbance of 1.0 at 280 nm during an incubation time of 30 min at pH 3.0 and 37°C Lateolabrax japonicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Hemoglobin + H2O
-
 Lateolabrax japonicus ?
-
 ?
additional information the cleavage site of isoform PG1 to produce pepsin is clearly at the 41-42 bond of Phe-Ala Lateolabrax japonicus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, pepsin isoform P1, SDS-PAGE Lateolabrax japonicus
? x * 35000, pepsinogen isoform PG1, SDS-PAGE Lateolabrax japonicus

Synonyms

Synonyms Comment Organism
pepsin 1 pepsin A isoform 1 Lateolabrax japonicus
PG1 pepsinogen isoform Lateolabrax japonicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Lateolabrax japonicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 at 50°C and pH 7.0, the half-life of isoform P1 is 35 min Lateolabrax japonicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.34
-
 hemoglobin isoform pepsin 1, in 250 mM sodium acetate buffer (pH 3.0), at 37°C Lateolabrax japonicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
-
 Lateolabrax japonicus

pI Value

Organism Comment pI Value Maximum pI Value
Lateolabrax japonicus pepsinogen isoform PG1, isoelectric focusing
-
 5.3

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
130
-
 hemoglobin isoform pepsin 1, in 250 mM sodium acetate buffer (pH 3.0), at 37°C Lateolabrax japonicus