3.2.1.23: beta-galactosidase

This is an abbreviated version!
For detailed information about beta-galactosidase, go to the full flat file.

Reaction

Synonyms

Aabeta-gal, Acid beta-galactosidase, B-GAL, BbgI, BbgII, BbgIII, BbgIV, beta galactosidase, beta-D-galactohydrolase, beta-D-galactosidase, beta-D-galactoside galactohydrolase, beta-D-glactanase, beta-D-lactosidase, beta-gal, beta-Gal 1, beta-Gal 2, beta-Gal II, beta-galactosidase, beta-galactosidase I, beta-galactosidase II, beta-galactosidase III, beta-galactosidase IV, beta-galase, beta-lactosidase, betagal, betaGly4, betaGS, BGA, BgaB, BgaBM, bgaD, BgaH, BGAL, BGAL1, BGAL10, BGAL11, BGAL12, BGAL13, BGAL14, BGAL15, BGAL16, BGAL17, BGAL2, BGAL3, BGAL4, BGAL5, BGAL6, BGAL7, BGAL8, BGAL9, BgalA, BgAP, BgaS, BgaX, BGL1, BglAp, BGT I, BGT II, cold-active beta-galactosidase, CTP-beta-gal, driselase, EABase, endo-beta-galactosidase, Exo-(1-->4)-beta-D-galactanase, exo-beta-(1->3)-D-galactanase, Gal-2, Gal-5, GAL1, Gal2, Gal3, Gal4, GALA, galactanase, galactosidase, galactosidase, beta, GALB, gherkin lactase, H-BgaS, hcbetagal, Hlac_2868, Hydrolact, LacA, LACS, lactase, lactase phlorizin hydrolase, lactosylceramidase II, Lactozym, Lactozym 3000L, LPH, Maxilact, Maxilact-L/2000, MeBglD2, More, ONPGase, Oryzatym, p-nitrophenyl beta-galactosidase, PF1208, PRGH1, S 2107, SA-beta-GAL, SA-betagal, senescence-associated beta-galactosidase, SPD_0065 protein, SR12 protein, Ss beta-gal, SSO3019, SSU0587, Sumiklat, Trilactase, XC1214, XG-specific beta-galactosidase, YesZ, YH4502, ZD410

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.23 beta-galactosidase

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Results	in table
64	Activating Compound
63272	AA Sequence
89	Application
1	CAS Registry Number
83	Cloned(Commentary)
11	Crystallization (Commentary)
6802	Disease/ Diagnostics
25	Expression
10	General Information
31	General Stability
2	IC50 Value
577	Inhibitors
115	kcat/KM [mM/s]
135	Ki Value [mM]
410	KM Value [mM]
65	Localization
205	Metals/Ions
251	Molecular Weight [Da]
143	Natural Substrates/ Products (Substrates)
42	Organic Solvent Stability
728	Organism
2	Oxidation Stability
9	Pathway
609	PDB ID
267	pH Optimum
49	pH Range
96	pH Stability
42	pI Value
34	Posttranslational Modification
90	Protein Variants
154	Purification (Commentary)
11	Reaction
5	Reaction Type
595	Reference
5	Renatured (Commentary)
117	Source Tissue
178	Specific Activity [micromol/min/mg]
35	Storage Stability
1115	Substrates and Products (Substrate)
189	Subunits
341	Synonyms
1	Systematic Name
208	Temperature Optimum [°C]
55	Temperature Range [°C]
260	Temperature Stability [°C]
177	Turnover Number [1/s]

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.17 - 1.75

2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside

4 entries

2 - 540

2-nitrophenyl beta-D-galactopyranoside

15 entries

179.5 - 224.9

2-nitrophenyl beta-D-galactoside

2 entries

1345

2-nitrophenyl-beta-D-fucopyranoside

Alicyclobacillus acidocaldarius

Q0R5R5

pH 6.5, 65°C, wild-type enzyme

678474

0.01 - 8559

2-nitrophenyl-beta-D-galactopyranoside

30 entries

8559

2-nitrophenyl-beta-D-galactoside

Kluyveromyces marxianus

P00723, Q6QTF4

pH 6.8

677630

2.6

3,4-dinitrophenyl beta-D-galactopyranoside

Xanthomonas phaseoli pv. manihotis

-

pH 7.0, 30°C, mutant E184A, glycohydrolase activity

679834

1.7

4-methylumbelliferyl beta-D-galactoside

Tenebrio molitor

-

-

655727

12.71 - 600

4-nitrophenyl beta-D-fucopyranoside

7 entries

5.4 - 620

4-nitrophenyl beta-D-galactopyranoside

14 entries

10.39 - 286

4-nitrophenyl beta-D-glucopyranoside

7 entries

1740

4-nitrophenyl-alpha-L-arabinopyranoside

Alicyclobacillus acidocaldarius

Q0R5R5

pH 6.5, 65°C, wild-type enzyme

678474

2.1 - 270000

4-nitrophenyl-beta-D-galactopyranoside

8 entries

2700000

4-nitrophenyl-beta-D-galactoside

Kluyveromyces marxianus

P00723, Q6QTF4

pH 6.8

677630

65

alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc

Aspergillus oryzae

Q2UCU3

pH 4.5, 60°C

750529

289

beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-beta-D-Glc

Aspergillus oryzae

Q2UCU3

pH 4.5, 60°C

750529

26

beta-D-galactosyl azide

Escherichia coli

-

-

171271

18

beta-lactose

Thermomyces lanuginosus

-

-

171344

6.95 - 8.12

cellobiose

2 entries

1.2 - 6300

lactose

45 entries

33 - 380

lactulose

2 entries

242

methyl-beta-D-galactopyranoside

Saccharolobus solfataricus

-

pH 6.5, 30°C

679616

16

methyl-beta-D-galactoside

Thermomyces lanuginosus

-

-

171344

43 - 603

o-nitrophenyl beta-D-galactopyranoside

10 entries

1 - 941

o-nitrophenyl-beta-D-galactoside

10 entries

2.4

p-nitrophenyl beta-D-fucoside

Tenebrio molitor

-

-

655727

5000

p-nitrophenyl-alpha-L-arabinoside

Thermomyces lanuginosus

-

-

171344

42.4 - 1040

p-nitrophenyl-beta-D-galactoside

4 entries

166

phenyl-beta-D-galactopyranoside

Saccharolobus solfataricus

-

pH 6.5, 30°C

679616

65

phenyl-beta-D-galactoside

Thermomyces lanuginosus

-

-

171344

additional information

additional information

Escherichia coli

-

turnover numbers of chimeric galactosidases

171341

-

0.17

2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside

Clostridium perfringens

-

mutant enzyme E354A, in 20 mM sodium acetate buffer at pH 6.0, 37°C

702342

1.35

2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside

Clostridium perfringens

-

mutant enzyme D429A, in 20 mM sodium acetate buffer at pH 6.0, 37°C

702342

1.55

2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside

Clostridium perfringens

-

mutant enzyme E467A, in 20 mM sodium acetate buffer at pH 6.0, 37°C

702342

1.75

2,4-dinitrophenyl 2-acetamido-2-deoxy-alpha-D-galactopyranosyl-(1-3)-[alpha-L-fucopyranosyl-(1-2)]-beta-D-galactopyranoside

Clostridium perfringens

-

wild type enzyme, in 20 mM sodium acetate buffer at pH 6.0, 37°C

702342

2 - 3.7

2-nitrophenyl beta-D-galactopyranoside

uncultured soil bacterium

-

pH 7, 5°C

713883

4.9

2-nitrophenyl beta-D-galactopyranoside

Escherichia coli

P00722

mutant enzyme H418N, in 30 mM TES buffer, 0.01 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C

706624

5.4

2-nitrophenyl beta-D-galactopyranoside

Escherichia coli

P00722

mutant enzyme H418E, in 30 mM TES buffer, 0.01 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C

706624

10.4

2-nitrophenyl beta-D-galactopyranoside

Escherichia coli

P00722

mutant enzyme H418F, in 30 mM TES buffer, 10 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C

706624

22.5

2-nitrophenyl beta-D-galactopyranoside

Lactiplantibacillus plantarum

-

wild-type, pH 6.5, 40°C

744825

62.7

2-nitrophenyl beta-D-galactopyranoside

Lactiplantibacillus plantarum

-

mutant FMNP01Gal-2, pH 6.5, 40°C

744825

66.7

2-nitrophenyl beta-D-galactopyranoside

uncultured soil bacterium

-

pH 7, 10°C

713883

90

2-nitrophenyl beta-D-galactopyranoside

Escherichia coli

P00722

wild type enzyme, in 30 mM TES buffer, 145 mM NaCl, pH 7.0 at 25°C

706624

92

2-nitrophenyl beta-D-galactopyranoside

Lactiplantibacillus plantarum WCFS1

-

at pH 7.5 and 55°C

703072

149

2-nitrophenyl beta-D-galactopyranoside

Caldicellulosiruptor saccharolyticus

-

at pH 6.0 and 80°C

701857

149

2-nitrophenyl beta-D-galactopyranoside

uncultured soil bacterium

-

pH 7, 20°C

713883

234

2-nitrophenyl beta-D-galactopyranoside

Streptococcus thermophilus

-

pH 6.5, 25-40°C

716892

293.9

2-nitrophenyl beta-D-galactopyranoside

Pisum sativum

-

in 50 mM glycine-HCl, pH 3.2, at 37°C

704135

386

2-nitrophenyl beta-D-galactopyranoside

uncultured soil bacterium

-

pH 7, 38°C

713883

540

2-nitrophenyl beta-D-galactopyranoside

Lactiplantibacillus pentosus

-

pH 6.5, 30°C

714562

179.5

2-nitrophenyl beta-D-galactoside

Aspergillus oryzae

Q2UCU3

mutant enzyme W806F, at pH 5.0 and 60°C

750594

224.9

2-nitrophenyl beta-D-galactoside

Aspergillus oryzae

Q2UCU3

wild type enzyme, at pH 5.0 and 60°C

750594

0.01 - 0.08

2-nitrophenyl-beta-D-galactopyranoside

Bos taurus

-

25°C

682849

0.21

2-nitrophenyl-beta-D-galactopyranoside

Alicyclobacillus acidocaldarius

Q0R5R5

pH 6.5, 65°C, mutant E313G

678474

0.32

2-nitrophenyl-beta-D-galactopyranoside

Alicyclobacillus acidocaldarius

Q0R5R5

pH 6.5, 65°C, mutant E157G

678474

12.7

2-nitrophenyl-beta-D-galactopyranoside

Arthrobacter psychrolactophilus

Q08KN3

pH 8.0, 10°C, recombinant enzyme

682671

13

2-nitrophenyl-beta-D-galactopyranoside

Arthrobacter psychrolactophilus

-

-

701854

17.9

2-nitrophenyl-beta-D-galactopyranoside

Alicyclobacillus acidocaldarius

-

pH 6.0, 65°C

679560

45.4

2-nitrophenyl-beta-D-galactopyranoside

Kluyveromyces marxianus

-

dimeric enzyme, 25°C

682849

45.4

2-nitrophenyl-beta-D-galactopyranoside

Penicillium canescens

-

dimeric enzyme, 25°C

682849

47.2

2-nitrophenyl-beta-D-galactopyranoside

Lactobacillus delbrueckii

Q1G9Z4

wild type enzyme, in 100 mM sodium acetate buffer pH 5.5, at 45°C

706705

49.2

2-nitrophenyl-beta-D-galactopyranoside

Lactobacillus delbrueckii

Q1G9Z4

mutant enzyme E491A, in HEPES buffer pH 6.5, at 45°C

706705

50

2-nitrophenyl-beta-D-galactopyranoside

Lactobacillus acidophilus

-

-

701854

52.4

2-nitrophenyl-beta-D-galactopyranoside

Arthrobacter sp.

C7ASJ5

at 10°C

702907

54

2-nitrophenyl-beta-D-galactopyranoside

Escherichia coli

P00722

mutant enzyme E416Q

706614

71.2

2-nitrophenyl-beta-D-galactopyranoside

Arthrobacter sp.

C7ASJ5

at 55°C

702907

81

2-nitrophenyl-beta-D-galactopyranoside

Arthrobacter sp.

C7ASJ5

at 20°C

702907

81

2-nitrophenyl-beta-D-galactopyranoside

Escherichia coli

P00722

mutant enzyme E416V

706614

95

2-nitrophenyl-beta-D-galactopyranoside

Saccharolobus solfataricus

-

-

701854

96

2-nitrophenyl-beta-D-galactopyranoside

Aspergillus carbonarius

-

pH 5.5

695663

123.9

2-nitrophenyl-beta-D-galactopyranoside

Arthrobacter sp.

C7ASJ5

at 30°C

702907

149

2-nitrophenyl-beta-D-galactopyranoside

Caldicellulosiruptor saccharolyticus

-

-

701854

169.9

2-nitrophenyl-beta-D-galactopyranoside

Arthrobacter sp.

C7ASJ5

at 40°C

702907

180

2-nitrophenyl-beta-D-galactopyranoside

Escherichia coli

-

dimeric enzyme, 25°C

682849

212.4

2-nitrophenyl-beta-D-galactopyranoside

Arthrobacter sp.

C7ASJ5

at 50°C

702907

338

2-nitrophenyl-beta-D-galactopyranoside

Limosilactobacillus reuteri

-

-

701854

400

2-nitrophenyl-beta-D-galactopyranoside

Escherichia coli

-

tetrameric enzyme, 25°C

682849

440

2-nitrophenyl-beta-D-galactopyranoside

Aspergillus carbonarius

-

pH 5.5

695663

620

2-nitrophenyl-beta-D-galactopyranoside

Escherichia coli

P00722

native enzyme

706614

2657

2-nitrophenyl-beta-D-galactopyranoside

Alicyclobacillus acidocaldarius

Q0R5R5

pH 6.5, 65°C, wild-type enzyme

678474

5700

2-nitrophenyl-beta-D-galactopyranoside

Saccharolobus solfataricus

-

pH 6.5, 75°C

679616

8559

2-nitrophenyl-beta-D-galactopyranoside

Kluyveromyces marxianus

P00723, Q6QTF4

pH 6.8, 37°C

677630

12.71

4-nitrophenyl beta-D-fucopyranoside

Putranjiva roxburghii

A0A023MIF8

pH 4.6, 37°C, recombinant enzyme

751822

14.77

4-nitrophenyl beta-D-fucopyranoside

Putranjiva roxburghii

A0A023MIF8

pH 4.6, 37°C, native enzyme

751822

34.8

4-nitrophenyl beta-D-fucopyranoside

uncultured microorganism

A0A1E1FFN6

pH 6.0, 60°C, without additive

749691

117

4-nitrophenyl beta-D-fucopyranoside

uncultured microorganism

A0A1E1FFN6

pH 6.0, 60°C, additive: 250 mM D-galactose

749691

142

4-nitrophenyl beta-D-fucopyranoside

uncultured microorganism

A0A1E1FFN6

pH 6.0, 60°C, additive: 25 mM cellobiose

749691

486

4-nitrophenyl beta-D-fucopyranoside

uncultured microorganism

A0A1E1FFN6

pH 6.0, 60°C, additive: 250 mM D-glucose

749691

600

4-nitrophenyl beta-D-fucopyranoside

uncultured microorganism

A0A1E1FFN6

pH 6.0, 60°C, additive: 250 mM D-xylose

749691

5.4

4-nitrophenyl beta-D-galactopyranoside

Escherichia coli

P00722

mutant enzyme H418E, in 30 mM TES buffer, 0.01 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C

706624

5.5

4-nitrophenyl beta-D-galactopyranoside

Escherichia coli

P00722

mutant enzyme H418N, in 30 mM TES buffer, 0.01 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C

706624

5.82

4-nitrophenyl beta-D-galactopyranoside

Putranjiva roxburghii

A0A023MIF8

pH 4.6, 37°C, recombinant enzyme

751822

7.39

4-nitrophenyl beta-D-galactopyranoside

Putranjiva roxburghii

A0A023MIF8

pH 4.6, 37°C, native enzyme

751822

10.3

4-nitrophenyl beta-D-galactopyranoside

Escherichia coli

P00722

mutant enzyme H418F, in 30 mM TES buffer, 10 mM Mg2+, 150 mM Na+, pH 7.0 at 25°C

706624

32

4-nitrophenyl beta-D-galactopyranoside

Caldicellulosiruptor saccharolyticus

-

at pH 6.0 and 80°C

701857

56

4-nitrophenyl beta-D-galactopyranoside

Saccharopolyspora rectivirgula

-

-

701854

95.6

4-nitrophenyl beta-D-galactopyranoside

uncultured microorganism

A0A1E1FFN6

pH 6.0, 60°C, without additive

749691

128

4-nitrophenyl beta-D-galactopyranoside

uncultured microorganism

A0A1E1FFN6

pH 6.0, 60°C, additive: 250 mM D-galactose

749691

129

4-nitrophenyl beta-D-galactopyranoside

uncultured microorganism

A0A1E1FFN6

pH 6.0, 60°C, additive: 25 mM cellobiose

749691

187

4-nitrophenyl beta-D-galactopyranoside

uncultured microorganism

A0A1E1FFN6

pH 6.0, 60°C, additive: 250 mM D-glucose

749691

197

4-nitrophenyl beta-D-galactopyranoside

uncultured microorganism

A0A1E1FFN6

pH 6.0, 60°C, additive: 250 mM D-xylose

749691

525

4-nitrophenyl beta-D-galactopyranoside

Aspergillus oryzae

Q2UCU3

pH 4.5, 60°C

750529

620

4-nitrophenyl beta-D-galactopyranoside

Escherichia coli

P00722

wild type enzyme, in 30 mM TES buffer, 145 mM NaCl, pH 7.0 at 25°C

706624

10.39

4-nitrophenyl beta-D-glucopyranoside

Putranjiva roxburghii

A0A023MIF8

pH 4.6, 37°C, recombinant enzyme

751822

12

4-nitrophenyl beta-D-glucopyranoside

Putranjiva roxburghii

A0A023MIF8

pH 4.6, 37°C, native enzyme

751822

31.2

4-nitrophenyl beta-D-glucopyranoside

uncultured microorganism

A0A1E1FFN6

pH 6.0, 60°C, without additive

749691

91.1

4-nitrophenyl beta-D-glucopyranoside

uncultured microorganism

A0A1E1FFN6

pH 6.0, 60°C, additive: 25 mM cellobiose

749691

108

4-nitrophenyl beta-D-glucopyranoside

uncultured microorganism

A0A1E1FFN6

pH 6.0, 60°C, additive: 250 mM D-galactose

749691

236

4-nitrophenyl beta-D-glucopyranoside

uncultured microorganism

A0A1E1FFN6

pH 6.0, 60°C, additive: 250 mM D-glucose

749691

286

4-nitrophenyl beta-D-glucopyranoside

uncultured microorganism

A0A1E1FFN6

pH 6.0, 60°C, additive: 250 mM D-xylose

749691

2.1

4-nitrophenyl-beta-D-galactopyranoside

Xanthomonas phaseoli pv. manihotis

-

pH 7.0, 30°C, mutant E184A, glycohydrolase activity

679834

32

4-nitrophenyl-beta-D-galactopyranoside

Caldicellulosiruptor saccharolyticus

-

-

701854

932

4-nitrophenyl-beta-D-galactopyranoside

Aspergillus aculeatus

-

-

701854

2140

4-nitrophenyl-beta-D-galactopyranoside

Xanthomonas phaseoli pv. manihotis

-

pH 7.0, 30°C, wild-type enzyme, glycohydrolase activity

679834

2988

4-nitrophenyl-beta-D-galactopyranoside

Alicyclobacillus acidocaldarius

Q0R5R5

pH 6.5, 65°C, wild-type enzyme

678474

15750

4-nitrophenyl-beta-D-galactopyranoside

Aspergillus carbonarius

-

pH 5.5

695663

74000

4-nitrophenyl-beta-D-galactopyranoside

Aspergillus carbonarius

-

pH 5.5

695663

270000

4-nitrophenyl-beta-D-galactopyranoside

Kluyveromyces marxianus

P00723, Q6QTF4

pH 6.8, 37°C

677630

6.95

cellobiose

Putranjiva roxburghii

A0A023MIF8

pH 4.6, 37°C, recombinant enzyme

751822

8.12

cellobiose

Putranjiva roxburghii

A0A023MIF8

pH 4.6, 37°C, native enzyme

751822

1.2

lactose

Tenebrio molitor

-

-

655727

1.76

lactose

Arthrobacter sp.

C7ASJ5

at 10°C

702907

1.9

lactose

Saccharolobus solfataricus

-

-

701854

2.36

lactose

Arthrobacter sp.

C7ASJ5

at 20°C

702907

2.67

lactose

Saccharolobus solfataricus

P22498

pH 7.5, 80°C, recombinant mutant enzyme F441Y

725811

3

lactose

Arthrobacter psychrolactophilus

-

-

701854

3.02

lactose

Arthrobacter psychrolactophilus

Q08KN3

pH 8.0, 10°C, recombinant enzyme

682671

4.1

lactose

Arthrobacter sp.

-

15°C, recombinant mutant BgaS7, i.e. E229D/V405A

679711

4.7

lactose

Arthrobacter sp.

Q7WTU5

at 5°C

655860

4.81

lactose

Arthrobacter sp.

C7ASJ5

at 30°C

702907

5.2

lactose

Arthrobacter sp.

Q7WTU5

at 18°C

655860

5.3

lactose

Arthrobacter sp.

-

15°C, recombinant wild-type BgaS

679711

5.73

lactose

Arthrobacter sp.

C7ASJ5

at 40°C

702907

6.98

lactose

Arthrobacter sp.

C7ASJ5

at 50°C

702907

12.5

lactose

Thermus sp.

-

-

171336

13

lactose

Aspergillus carbonarius

-

pH 5.5

695663

13

lactose

Limosilactobacillus reuteri

-

pH 6.5, 30°C, strain L103

665287

18.7

lactose

Arthrobacter sp.

-

15°C, recombinant mutant BgaS6, i.e.E229D/V405A/G803D

679711

20

lactose

Lactiplantibacillus pentosus

-

pH 6.5, 30°C

714562

33

lactose

Pseudoalteromonas haloplanktis

P81650

25°C

654252

33

lactose

uncultured soil bacterium

-

pH 7, 10°C

713883

42

lactose

Caldicellulosiruptor saccharolyticus

-

-

701854

42

lactose

Caldicellulosiruptor saccharolyticus

-

at pH 6.0 and 80°C

701857

45.7

lactose

Lactobacillus delbrueckii

Q1G9Z4

wild type enzyme, in 100 mM sodium acetate buffer pH 5.5, at 45°C

706705

46.3

lactose

Lactobacillus delbrueckii

Q1G9Z4

mutant enzyme E491A, in HEPES buffer pH 6.5, at 45°C

706705

53

lactose

uncultured soil bacterium

-

pH 7, 5°C

713883

55

lactose

Aspergillus carbonarius

-

pH 5.5

695663

55.1

lactose

Pisum sativum

-

in 50 mM sodium acetate-acetic acid, pH 4.0, at 37°C

704135

58

lactose

Limosilactobacillus reuteri

-

-

701854

58

lactose

Limosilactobacillus reuteri

-

pH 6.5, 30°C, strain L461

665287

63

lactose

Saccharopolyspora rectivirgula

-

-

701854

63.1

lactose

Saccharopolyspora rectivirgula

-

-

171353

92

lactose

uncultured soil bacterium

-

pH 7, 20°C

713883

98

lactose

Lactiplantibacillus plantarum WCFS1

-

at pH 7.0 and 60°C

703072

114

lactose

Saccharolobus solfataricus

-

pH 6.5, 30°C

679616

122.6

lactose

Saccharolobus solfataricus

P22498

30°C, pH 6.5, wild-type enzyme

722308

141

lactose

uncultured soil bacterium

-

pH 7, 38°C

713883

162.2

lactose

Saccharolobus solfataricus

P22498

30°C, pH 6.5, mutant enzyme F441Y

722308

167.5

lactose

Saccharolobus solfataricus

P22498

30°C, pH 6.5, mutant enzyme F359Q

722308

212

lactose

Alicyclobacillus acidocaldarius

Q0R5R5

pH 6.5, 65°C, wild-type enzyme

678474

254

lactose

Aspergillus aculeatus

-

-

701854

632

lactose

Lactobacillus acidophilus

-

-

701854

869

lactose

Aspergillus oryzae

Q2UCU3

pH 4.5, 60°C

750529

6300

lactose

Kluyveromyces marxianus

P00723, Q6QTF4

pH 6.8

677630

6300

lactose

Kluyveromyces marxianus

P00723, Q6QTF4

pH 6.8, 37°C

677630

33

lactulose

Thermomyces lanuginosus

-

-

171344

380

lactulose

Alicyclobacillus acidocaldarius

Q0R5R5

pH 6.5, 65°C, wild-type enzyme

678474

43

o-nitrophenyl beta-D-galactopyranoside

Arthrobacter sp.

Q7WTU5

at 5°C

655860

57.2

o-nitrophenyl beta-D-galactopyranoside

Arthrobacter sp.

Q7WTU5

at 10°C

655860

81.8

o-nitrophenyl beta-D-galactopyranoside

Planococcus sp. (in: Bacteria)

Q9KI47

1.9°C

654248

100

o-nitrophenyl beta-D-galactopyranoside

Arthrobacter sp.

Q7WTU5

at 18°C

655860

104

o-nitrophenyl beta-D-galactopyranoside

Planococcus sp. (in: Bacteria)

Q9KI47

10°C

654248

110.1

o-nitrophenyl beta-D-galactopyranoside

Arthrobacter sp.

Q7WTU5

at 20°C

655860

203

o-nitrophenyl beta-D-galactopyranoside

Pseudoalteromonas haloplanktis

P81650

20°C

654252

288

o-nitrophenyl beta-D-galactopyranoside

Planococcus sp. (in: Bacteria)

Q9KI47

20°C

654248

507

o-nitrophenyl beta-D-galactopyranoside

Planococcus sp. (in: Bacteria)

Q9KI47

30°C

654248

603

o-nitrophenyl beta-D-galactopyranoside

Planococcus sp. (in: Bacteria)

Q9KI47

39°C

654248

1

o-nitrophenyl-beta-D-galactoside

Kluyveromyces lactis

-

isoenzyme betaG41

171290

5

o-nitrophenyl-beta-D-galactoside

Kluyveromyces lactis

-

isoenzyme betaG630

171290

47.6

o-nitrophenyl-beta-D-galactoside

Saccharopolyspora rectivirgula

-

-

171353

54.5

o-nitrophenyl-beta-D-galactoside

Thermoanaerobacterium thermosulfurigenes

-

at 25°C, in presence of 10 mM Mg2+

171346

66.7

o-nitrophenyl-beta-D-galactoside

Thermus sp.

-

-

171336

264

o-nitrophenyl-beta-D-galactoside

Thermoanaerobacterium thermosulfurigenes

-

at 50°C, in presence of 10 mM Mg2+

171346

338

o-nitrophenyl-beta-D-galactoside

Limosilactobacillus reuteri

-

pH 6.5, 30°C, strain L461

665287

431

o-nitrophenyl-beta-D-galactoside

Thermoanaerobacter sp.

-

-

171289

888

o-nitrophenyl-beta-D-galactoside

Kluyveromyces lactis

-

isoenzyme betaG550

171290

941

o-nitrophenyl-beta-D-galactoside

Thermomyces lanuginosus

-

-

171344

42.4

p-nitrophenyl-beta-D-galactoside

Thermoanaerobacterium thermosulfurigenes

-

at 25°C, in presence of 10 mM Mg2+

171346

55.8

p-nitrophenyl-beta-D-galactoside

Saccharopolyspora rectivirgula

-

-

171353

312

p-nitrophenyl-beta-D-galactoside

Thermoanaerobacterium thermosulfurigenes

-

at 50°C, in presence of 10 mM Mg2+

171346

1040

p-nitrophenyl-beta-D-galactoside

Thermomyces lanuginosus

-

-

171344