Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.2.1.23: beta-galactosidase

This is an abbreviated version!
For detailed information about beta-galactosidase, go to the full flat file.

Word Map on EC 3.2.1.23

Reaction

beta-D-galactopyranosyl-(1-4)-beta-D-galactopyranosyl-(1-6)-beta-D-galactopyranosyl-(1-3)-beta-D-galactopyranose
+ 3 H2O = 4 beta-D-galactopyranose

Synonyms

Aabeta-gal, Acid beta-galactosidase, B-GAL, BbgI, BbgII, BbgIII, BbgIV, beta galactosidase, beta-D-galactohydrolase, beta-D-galactosidase, beta-D-galactoside galactohydrolase, beta-D-glactanase, beta-D-lactosidase, beta-gal, beta-Gal 1, beta-Gal 2, beta-Gal II, beta-galactosidase, beta-galactosidase I, beta-galactosidase II, beta-galactosidase III, beta-galactosidase IV, beta-galase, beta-lactosidase, betagal, betaGly4, betaGS, BGA, BgaB, BgaBM, bgaD, BgaH, BGAL, BGAL1, BGAL10, BGAL11, BGAL12, BGAL13, BGAL14, BGAL15, BGAL16, BGAL17, BGAL2, BGAL3, BGAL4, BGAL5, BGAL6, BGAL7, BGAL8, BGAL9, BgalA, BgAP, BgaS, BgaX, BGL1, BglAp, BGT I, BGT II, cold-active beta-galactosidase, CTP-beta-gal, driselase, EABase, endo-beta-galactosidase, Exo-(1-->4)-beta-D-galactanase, exo-beta-(1->3)-D-galactanase, Gal-2, Gal-5, GAL1, Gal2, Gal3, Gal4, GALA, galactanase, galactosidase, galactosidase, beta, GALB, gherkin lactase, H-BgaS, hcbetagal, Hlac_2868, Hydrolact, LacA, LACS, lactase, lactase phlorizin hydrolase, lactosylceramidase II, Lactozym, Lactozym 3000L, LPH, Maxilact, Maxilact-L/2000, MeBglD2, More, ONPGase, Oryzatym, p-nitrophenyl beta-galactosidase, PF1208, PRGH1, S 2107, SA-beta-GAL, SA-betagal, senescence-associated beta-galactosidase, SPD_0065 protein, SR12 protein, Ss beta-gal, SSO3019, SSU0587, Sumiklat, Trilactase, XC1214, XG-specific beta-galactosidase, YesZ, YH4502, ZD410

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.23 beta-galactosidase

pH Stability

pH Stability on EC 3.2.1.23 - beta-galactosidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.5 - 12
the enzyme retains more than 50% of its initial activity after incubation at pH 0.5 through 12.0 for 60 min
704131
1 - 2
-
70°C, about 30% loss of activity after 10 min, about 50% loss of activity after 1 h, about 75% loss of activity after 3 h
655365
10
-
about 65% loss of activity after 10 in, about 90% loss of activity after 1 h
655365
2.5 - 7
2.5 - 8
-
stable
171190
2.6 - 7
-
at pH 2.6 free enzyme loses more than 30% activity after 24 h, while the immobilized enzyme (on Ca-alginate gel beads) retains almost the same activity, at pH 7.0 the free enzyme loses more than 90% activity after 24 h while the immobilized enzyme retains around 40% activity
701982
2.9 - 9.3
-
30°C, 60 min, stable
171342
3 - 10
3 - 5
3 - 5.5
-
kinetic modelling of the thermal and pH inactivation of the free and an immobilized form of the enzyme, inactivation mechanism, detailed overview
678007
3 - 6
-
37°C, 2 h, stable
171311
3 - 8
-
stable
171280
3.5
-
30°C, 10 min, more than 60% loss of activity
171330
3.5 - 7.5
3.5 - 8
-
stable
171280
3.5 - 8.5
-
4°C, 24 h, stable
171288
4 - 10
-
the enzymatic activity of encapsulated beta-Gal remains constant after pre-incubation at pH 4-10. However, while at pH 2 the soluble enzyme is completely inactivated, encapsulated beta-Gal retains 40% of its maximum activity
703289
4 - 8
4.4 - 8
4.5 - 6.7
-
37°C, maximal stability
171314
4.5 - 7
-
40°C, 3 h, stable
171325
5 - 7
-
30 min, stable
665014
5 - 8
-
pH 5.0: about 30% of maximal activity, pH 8.5: about 50% of maximal activity
171336
5 - 8.5
-
stable over the range
654292
5 - 9
5.5 - 7.5
over 50% of activity at pH 5.5-7.5 at 30°C
702907
5.5 - 8
-
the enzyme remains stable from pH 5.5-8.0, about 40% relative activity at pH 4.0 and 10.0, about 70% relative activity at pH 5.0 and 9.0, less than 10% activity at pH 11.0
701953
5.5 - 9
-
27°C, 10 min, more than 85% of maximal activity
171324
5.6
-
room temperature, 10 days, retains more than 90% of maximal activity
171303
6 - 10
6 - 6.5
-
enzyme is most stable between pH 6.0 and pH 6.5, strain L461
665287
6 - 7
6 - 7.2
-
rapid inactivation below pH 6.0 and above pH 7.2
171306
6 - 8
-
stable
171190
6 - 9
6.5
-
37°C, 1 h, stable only around
171305
6.5 - 10
stable
654252
6.5 - 11
-
enzyme immobilized on calcium alginate
664765
6.5 - 7
-
purified enzyme, stable
682989
6.5 - 7.5
6.5 - 8
-
-
703072
6.8
-
47°C, 2 h, stable
171344
8.5
-
entrapped cross-linked enzyme in complex with concanavalin A: 21% remaining activity, free enzyme: 7% remaining activity
680208
9 - 13
-
kinetic modelling of the thermal and pH inactivation of the free and an immobilized form of the enzyme, inactivation mechanism, detailed overview
678007
additional information