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Literature summary for 3.2.1.23 extracted from

  • Iqbal, S.; Nguyen, T.H.; Nguyen, T.T.; Maischberger, T.; Haltrich, D.
    beta-Galactosidase from Lactobacillus plantarum WCFS1: biochemical characterization and formation of prebiotic galacto-oligosaccharides (2010), Carbohydr. Res., 345, 1408-1416.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol the lower concentrations (1-10 mM) of 2-mercaptoethanol show a stimulating effect on activity Lactiplantibacillus plantarum WCFS1
dithiothreitol the lower concentrations (1-10 mM) of dithiothreitol show a stimulating effect on activity Lactiplantibacillus plantarum WCFS1
Urea the lower concentrations (1-10 mM) of urea show a stimulating effect on activity Lactiplantibacillus plantarum WCFS1

Inhibitors

Inhibitors Comment Organism Structure
beta-mercaptoethanol 100 mM of beta-mercaptoethanol shows an inhibitory effect on activity (91% residual activity) Lactiplantibacillus plantarum WCFS1
dithiothreitol 100 mM of dithiothreitol shows an inhibitory effect on activity (84% residual activity) Lactiplantibacillus plantarum WCFS1
EDTA EDTA strongly inactivates the enzyme even at 1 mM (17% residual activity), 13% residual activity at 100 mM EDTA Lactiplantibacillus plantarum WCFS1
Urea 100 mM of urea shows an inhibitory effect on activity (48% residual activity) Lactiplantibacillus plantarum WCFS1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.9
-
2-nitrophenyl beta-D-galactopyranoside at pH 7.5 and 55°C Lactiplantibacillus plantarum WCFS1
29
-
lactose at pH 7.0 and 60°C Lactiplantibacillus plantarum WCFS1

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 326% relative activity at 10 mM Lactiplantibacillus plantarum WCFS1
Mg2+ the enzyme needs MgCl2 for its stability retaining 73% and 85% of its activity after an incubation at 37°C for 5 h in the presence of 1 and 10 mM MgCl2, 414% relative activity at 10 mM Lactiplantibacillus plantarum WCFS1
Mn2+ 341% relative activity at 10 mM Lactiplantibacillus plantarum WCFS1
Na+ 165% relative activity at 10 mM Lactiplantibacillus plantarum WCFS1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
1 * 72000 + 1 * 35000, SDS-PAGE Lactiplantibacillus plantarum WCFS1
35223
-
1 * 72180 + 1 * 35223, calculated from amino acid sequence Lactiplantibacillus plantarum WCFS1
72000
-
1 * 72000 + 1 * 35000, SDS-PAGE Lactiplantibacillus plantarum WCFS1
72180
-
1 * 72180 + 1 * 35223, calculated from amino acid sequence Lactiplantibacillus plantarum WCFS1
107000
-
gel filtration Lactiplantibacillus plantarum WCFS1

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum WCFS1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
p-aminobenzyl 1-thio-beta-D-galactopyranoside column chromatography Lactiplantibacillus plantarum WCFS1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O preferred substrate Lactiplantibacillus plantarum WCFS1 2-nitrophenol + beta-D-galactose
-
?
lactose + H2O preferred substrate Lactiplantibacillus plantarum WCFS1 D-galactose + D-glucose
-
?
additional information the enzyme shows a strong preference for the formation of beta-(1-6) linkages in its transgalactosylation mode, while beta-(1-3)-linked products are formed to a lesser extent, comprising about 80% and 9%, respectively, of the newly formed glycosidic linkages in the oligosaccharide mixture at maximum galacto-oligosaccharides formation Lactiplantibacillus plantarum WCFS1 ?
-
?

Subunits

Subunits Comment Organism
heterodimer 1 * 72000 + 1 * 35000, SDS-PAGE Lactiplantibacillus plantarum WCFS1
heterodimer 1 * 72180 + 1 * 35223, calculated from amino acid sequence Lactiplantibacillus plantarum WCFS1

Synonyms

Synonyms Comment Organism
beta-D-galactoside galactohydrolase
-
Lactiplantibacillus plantarum WCFS1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
for hydrolysis of 2-nitrophenyl beta-D-galactopyranoside Lactiplantibacillus plantarum WCFS1
60
-
for hydrolysis of lactose Lactiplantibacillus plantarum WCFS1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
92
-
2-nitrophenyl beta-D-galactopyranoside at pH 7.5 and 55°C Lactiplantibacillus plantarum WCFS1
98
-
lactose at pH 7.0 and 60°C Lactiplantibacillus plantarum WCFS1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
for hydrolysis of lactose Lactiplantibacillus plantarum WCFS1
7.5
-
for hydrolysis of 2-nitrophenyl beta-D-galactopyranoside Lactiplantibacillus plantarum WCFS1

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 8
-
Lactiplantibacillus plantarum WCFS1

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.3
-
lactose at pH 7.0 and 60°C Lactiplantibacillus plantarum WCFS1
130
-
2-nitrophenyl beta-D-galactopyranoside at pH 7.5 and 55°C Lactiplantibacillus plantarum WCFS1