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3.2.1.23: beta-galactosidase

This is an abbreviated version!
For detailed information about beta-galactosidase, go to the full flat file.

Word Map on EC 3.2.1.23

Reaction

beta-D-galactopyranosyl-(1-4)-beta-D-galactopyranosyl-(1-6)-beta-D-galactopyranosyl-(1-3)-beta-D-galactopyranose
+ 3 H2O = 4 beta-D-galactopyranose

Synonyms

Aabeta-gal, Acid beta-galactosidase, B-GAL, BbgI, BbgII, BbgIII, BbgIV, beta galactosidase, beta-D-galactohydrolase, beta-D-galactosidase, beta-D-galactoside galactohydrolase, beta-D-glactanase, beta-D-lactosidase, beta-gal, beta-Gal 1, beta-Gal 2, beta-Gal II, beta-galactosidase, beta-galactosidase I, beta-galactosidase II, beta-galactosidase III, beta-galactosidase IV, beta-galase, beta-lactosidase, betagal, betaGly4, betaGS, BGA, BgaB, BgaBM, bgaD, BgaH, BGAL, BGAL1, BGAL10, BGAL11, BGAL12, BGAL13, BGAL14, BGAL15, BGAL16, BGAL17, BGAL2, BGAL3, BGAL4, BGAL5, BGAL6, BGAL7, BGAL8, BGAL9, BgalA, BgAP, BgaS, BgaX, BGL1, BglAp, BGT I, BGT II, cold-active beta-galactosidase, CTP-beta-gal, driselase, EABase, endo-beta-galactosidase, Exo-(1-->4)-beta-D-galactanase, exo-beta-(1->3)-D-galactanase, Gal-2, Gal-5, GAL1, Gal2, Gal3, Gal4, GALA, galactanase, galactosidase, galactosidase, beta, GALB, gherkin lactase, H-BgaS, hcbetagal, Hlac_2868, Hydrolact, LacA, LACS, lactase, lactase phlorizin hydrolase, lactosylceramidase II, Lactozym, Lactozym 3000L, LPH, Maxilact, Maxilact-L/2000, MeBglD2, More, ONPGase, Oryzatym, p-nitrophenyl beta-galactosidase, PF1208, PRGH1, S 2107, SA-beta-GAL, SA-betagal, senescence-associated beta-galactosidase, SPD_0065 protein, SR12 protein, Ss beta-gal, SSO3019, SSU0587, Sumiklat, Trilactase, XC1214, XG-specific beta-galactosidase, YesZ, YH4502, ZD410

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.23 beta-galactosidase

Storage Stability

Storage Stability on EC 3.2.1.23 - beta-galactosidase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10°C, stable for several months
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-20°C, 20 mM phosphate buffer, pH 6.9 without the addition of protein stabilizers, at least 2 years, no loss of activity
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-20°C, 30% glycerol, stable
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-20°C, 50% loss of activity when stored overnight
-20°C, complete inactivation after a few days
-
-20°C, crude enzyme extract, 24 h, fully stable, loss of 49% activity after 12 days, partially purified enzyme is stable for 60 days
-
-20°C, purified enzyme, 50 mM sodium phosphate, pH 6.5, and 2.8 mM 4-nitrophenyl-beta-D-galactopyranoside, stable for long periods of storage
-
-20°C, stable for at least 2 months
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-20°C, stable for at least 6 months
-
0-4°C, 0.2-0.3 mg of protein per ml, 10 mM Tris/HCl buffer, 0.1 M NaCl, 0.02% sodium azide, both enzyme forms, stable for 3 months
-
22°C, purified enzyme, half-life is 180 h
25°C, 20 mM phosphate buffer, pH 6.9 without the addition of protein stabilizers, 55 days, no loss of activity
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25°C, pH 6.5, more than 60% activity after 45 days
2°C, stable for several months
-
37°C, purified enzyme, half-life is 120 h
45°C, purified enzyme, half-life is 24 h
4°C, 18% loss of activity when stored overnight
4°C, 72 h, stable
-
4°C, beta-D-galactoside galactohydrolase pH 4.2, stable for 30 days
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4°C, crude preparation, 1 week, the enzyme remains fairly stable with a residual activity of more than 60%
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4°C, free enzyme in phosphate buffer (0.05 M, pH 6.5), 30 days, about 90% loss of activity
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4°C, immobilized enzyme (on Ca-alginate gel beads) in 0.045 M CaCl2 aqueous solution, 30 days, about 10% loss of activity
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4°C, immobilized enzymes, stable
4°C, lyophilized preparation, stable for 6 months
4°C, pH 4.0, 50 mM sodium citrate buffer, stable for several months
-
4°C, pH 6.0-9.0, stable for 24 h
4°C, protein concentration 1.2 mg/ml, stable for 8 months
-
4°C, purified enzyme, completely stable for 30 days, 90% remaining activity after 45 days,
4°C, stable for several months
-
5°C to 7°C, 10% v/v gycerol, 20% loss of activity after 4 weeks, complete inactivation in absence of glycerol
Bacteroides polypragmatus
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entrapped crosslinked concanavalin A-beta galactosidase complex retains 95% activity after sevenfold repeated use, and 93% of its original activity after 2 months storage at 4°C
-