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Literature summary for 3.2.1.23 extracted from

  • Ferreira, A.H.; Terra, W.R.; Ferreira, C.
    Characterization of a beta-glycosidase highly active on disaccharides and of a beta-galactosidase from Tenebrio molitor midgut lumen (2003), Insect Biochem. Mol. Biol., 33, 253-265.
    View publication on PubMed
Search for more literature for 3.2.1.23

Inhibitors

Inhibitors Comment Organism Structure
gamma-galactonolactone
-
 Tenebrio molitor
lactose
-
 Tenebrio molitor
octyl-beta-thio-galactoside
-
 Tenebrio molitor
p-nitrophenyl beta-D-fucoside
-
 Tenebrio molitor
p-nitrophenyl beta-D-galactopyranoside
-
 Tenebrio molitor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 4-methylumbelliferyl beta-D-galactoside
-
 Tenebrio molitor
21
-
 p-nitrophenyl beta-D-fucoside
-
 Tenebrio molitor
250
-
 lactose
-
 Tenebrio molitor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 2 * 30000, SDS-PAGE Tenebrio molitor
64000
-
 gel filtration Tenebrio molitor

Organism

Organism UniProt Comment Textmining
Tenebrio molitor
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Tenebrio molitor

Source Tissue

Source Tissue Comment Organism Textmining
larva midgut Tenebrio molitor
-
midgut lumen Tenebrio molitor
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.7
-
-
 Tenebrio molitor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl beta-D-galactoside + H2O
-
 Tenebrio molitor 4-methylumbelliferone + beta-D-galactose
-
 ?
lactose + H2O
-
 Tenebrio molitor D-glucose + D-galactose
-
 ?
p-nitrophenyl beta-D-fucoside + H2O
-
 Tenebrio molitor p-nitrophenol + beta-D-fucose
-
 ?
p-nitrophenyl beta-D-galactopyranoside + H2O
-
 Tenebrio molitor p-nitrophenol + beta-D-galactose
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 30000, SDS-PAGE Tenebrio molitor

Synonyms

Synonyms Comment Organism
betaGly4
-
 Tenebrio molitor

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 pH 5.5, more than 80% loss of activity after 430 min Tenebrio molitor

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2
-
 lactose
-
 Tenebrio molitor
1.7
-
 4-methylumbelliferyl beta-D-galactoside
-
 Tenebrio molitor
2.4
-
 p-nitrophenyl beta-D-fucoside
-
 Tenebrio molitor

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0081
-
 gamma-galactonolactone hydrolysis of 4-methylumbelliferyl beta-D-galactoside Tenebrio molitor
0.02
-
 gamma-galactonolactone hydrolysis of p-nitrophenyl beta-D-galactoside Tenebrio molitor
0.34
-
 octyl-beta-thio-galactoside hydrolysis of 4-methylumbelliferyl beta-D-galactoside Tenebrio molitor
0.7
-
 p-nitrophenyl beta-D-galactopyranoside hydrolysis of 4-methylumbellipheryl beta-D-galactoside Tenebrio molitor
1
-
 octyl-beta-thio-galactoside hydrolysis of p-nitrophenyl beta-D-galactoside Tenebrio molitor
4.3
-
 p-nitrophenyl beta-D-fucoside hydrolysis of 4-methylumbelliferyl beta-D-galactoside Tenebrio molitor
260
-
 lactose hydrolysis of 4-methylumbelliferyl beta-D-galactoside Tenebrio molitor