Any feedback?
Literature summary for 3.2.1.23 extracted from
- A beta-galactosidase from pea seeds (PsBGAL): Purification, stabilization, catalytic energetics, conformational heterogeneity, and its significance (2009), J. Agric. Food Chem., 57, 7086-7096.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cetyltrimethylammonium bromide | 148% relative activity at 0.25% (v/v) | Pisum sativum |  |
| EDTA | 127.3% relative activity at 0.02 mM | Pisum sativum | |
| NaCl | 123.47% relative activity at 50 mM | Pisum sativum | |
| NaF | 132.65% relative activity at 10 mM | Pisum sativum | |
General Stability
| General Stability | Organism |
|---|---|
| dilute purified BGAL preparation with a protein concentration less than 0.1 mg/ml is unstable with a t1/2 of about 13 h at 4°C and pH 5.0, at a protein concentration of 0.1 mg/ml the enzyme has a t1/2 of about 10 days at 4°C and pH 5.0 | Pisum sativum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 88.29% residual activity at 1 mM | Pisum sativum | |
| D-galactose | 50% residual activity at 10 mM | Pisum sativum | |
| Mg2+ | 85.18% residual activity at 1 mM | Pisum sativum | |
| additional information | not affected by methylmannoside | Pisum sativum | |
| SDS | 12.69% residual activity at 0.1% (w/v) | Pisum sativum | |
| Triton X-100 | 61% residual activity at 10% (v/v) | Pisum sativum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.97 | - |
2-nitrophenyl beta-D-galactopyranoside | in 50 mM glycine-HCl, pH 3.2, at 37°C | Pisum sativum | |
| 3.03 | - |
lactose | in 50 mM sodium acetate-acetic acid, pH 4.0, at 37°C | Pisum sativum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54200 | - |
deglycosylated enzyme, SDS-PAGE | Pisum sativum |
| 55000 | - |
purified enzyme, SDS-PAGE | Pisum sativum |
| 228000 | - |
purified enzyme, at pH 5.0, gel filtration | Pisum sativum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Pisum sativum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, octyl Sepharose column chromatography, and DEAE-Sephacel gel filtration | Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Pisum sativum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.085 | - |
crude extract, at 37°C | Pisum sativum |
| 77.33 | - |
after 910fold purification, at 37°C | Pisum sativum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, crude preparation, 1 week, the enzyme remains fairly stable with a residual activity of more than 60% | Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-nitrophenyl alpha-D-galactopyranoside + H2O | 1.62% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | 2-nitrophenol + alpha-D-galactopyranose | - |
? | |
| 2-nitrophenyl beta-D-galactopyranoside + H2O | 100% activity | Pisum sativum | 2-nitrophenol + beta-D-galactopyranose | - |
? | |
| 2-nitrophenyl beta-D-glucopyranoside + H2O | 3.27% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | 2-nitrophenol + D-glucopyranose | - |
? | |
| 2-nitrophenyl-alpha-D-mannopyranoside + H2O | 0.31% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | 2-nitrophenol + alpha-D-mannopyranose | - |
? | |
| 3-nitrophenyl beta-D-galactopyranoside + H2O | 0.13% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | 3-nitrophenol + beta-D-galactopyranose | - |
? | |
| 4-nitrophenyl beta-D-galactopyranoside + H2O | 34.49% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | 0.47% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | 4-nitrophenol + D-glucopyranose | - |
? | |
| 4-nitrophenyl beta-D-glucuronide + H2O | 0.69% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | 4-nitrophenol + beta-D-glucuronic acid | - |
? | |
| 4-nitrophenyl beta-D-mannopyranoside + H2O | 0.9% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | 4-nitrophenol + beta-D-mannopyranose | - |
? | |
| 4-nitrophenyl beta-D-xylopyranoside + H2O | 0.31% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
| 4-nitrophenyl beta-L-arabinoside + H2O | 0.21% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | 4-nitrophenol + beta-L-arabinose | - |
? | |
| 4-nitrophenyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O | 1.18% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | 4-nitrophenol + 2-acetamido-2-deoxy-beta-D-glucopyranose | - |
? | |
| 4-nitrophenyl-alpha-D-galactopyranoside + H2O | 0.51% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | 4-nitrophenol + alpha-D-galactopyranose | - |
? | |
| 4-nitrophenyl-alpha-D-galactopyranoside + H2O | 0.9% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | 4-nitrophenol + alpha-D-galactopyranose | - |
? | |
| 4-nitrophenyl-alpha-L-arabinoside + H2O | 0.74% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | 4-nitrophenol + alpha-L-arabinose | - |
? | |
| lactose + H2O | 11.7% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | D-glucose + D-galactose | - |
? | |
| pectic polysaccharides + H2O | 1.27% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | ? | - |
? | |
| xyloglucan + H2O | structural xyloglucans show 0.57% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside, reserve xyloglucans show 1.8% relative activity compared to 2-nitrophenyl-beta-D-galactopyranoside | Pisum sativum | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BGAL | - |
Pisum sativum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Pisum sativum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 60 | the enzyme retains 86.26% of its activity when kept for 10 min at 40°C however it loses 90.85% of its activity at 50°C and 94.89% at 60°C | Pisum sativum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 55.1 | - |
lactose | in 50 mM sodium acetate-acetic acid, pH 4.0, at 37°C | Pisum sativum | |
| 293.9 | - |
2-nitrophenyl beta-D-galactopyranoside | in 50 mM glycine-HCl, pH 3.2, at 37°C | Pisum sativum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.2 | - |
for hydrolysis of 2-nitrophenyl beta-D-galactopyranoside | Pisum sativum |
| 4 | - |
for hydrolysis of lactose | Pisum sativum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.5 | - |
D-galactose | - |
Pisum sativum | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pisum sativum | purified enzyme, isoelectric focusing | - |
8.8 |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 10 | - |
- |
Pisum sativum | D-galactose | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 18.18 | - |
lactose | in 50 mM sodium acetate-acetic acid, pH 4.0, at 37°C | Pisum sativum | |
| 302.9 | - |
2-nitrophenyl beta-D-galactopyranoside | in 50 mM glycine-HCl, pH 3.2, at 37°C | Pisum sativum | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
