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3.2.1.23: beta-galactosidase

This is an abbreviated version!
For detailed information about beta-galactosidase, go to the full flat file.

Word Map on EC 3.2.1.23

Reaction

beta-D-galactopyranosyl-(1-4)-beta-D-galactopyranosyl-(1-6)-beta-D-galactopyranosyl-(1-3)-beta-D-galactopyranose
+ 3 H2O = 4 beta-D-galactopyranose

Synonyms

Aabeta-gal, Acid beta-galactosidase, B-GAL, BbgI, BbgII, BbgIII, BbgIV, beta galactosidase, beta-D-galactohydrolase, beta-D-galactosidase, beta-D-galactoside galactohydrolase, beta-D-glactanase, beta-D-lactosidase, beta-gal, beta-Gal 1, beta-Gal 2, beta-Gal II, beta-galactosidase, beta-galactosidase I, beta-galactosidase II, beta-galactosidase III, beta-galactosidase IV, beta-galase, beta-lactosidase, betagal, betaGly4, betaGS, BGA, BgaB, BgaBM, bgaD, BgaH, BGAL, BGAL1, BGAL10, BGAL11, BGAL12, BGAL13, BGAL14, BGAL15, BGAL16, BGAL17, BGAL2, BGAL3, BGAL4, BGAL5, BGAL6, BGAL7, BGAL8, BGAL9, BgalA, BgAP, BgaS, BgaX, BGL1, BglAp, BGT I, BGT II, cold-active beta-galactosidase, CTP-beta-gal, driselase, EABase, endo-beta-galactosidase, Exo-(1-->4)-beta-D-galactanase, exo-beta-(1->3)-D-galactanase, Gal-2, Gal-5, GAL1, Gal2, Gal3, Gal4, GALA, galactanase, galactosidase, galactosidase, beta, GALB, gherkin lactase, H-BgaS, hcbetagal, Hlac_2868, Hydrolact, LacA, LACS, lactase, lactase phlorizin hydrolase, lactosylceramidase II, Lactozym, Lactozym 3000L, LPH, Maxilact, Maxilact-L/2000, MeBglD2, More, ONPGase, Oryzatym, p-nitrophenyl beta-galactosidase, PF1208, PRGH1, S 2107, SA-beta-GAL, SA-betagal, senescence-associated beta-galactosidase, SPD_0065 protein, SR12 protein, Ss beta-gal, SSO3019, SSU0587, Sumiklat, Trilactase, XC1214, XG-specific beta-galactosidase, YesZ, YH4502, ZD410

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.23 beta-galactosidase

Specific Activity

Specific Activity on EC 3.2.1.23 - beta-galactosidase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.012
recombinant isozyme BGal1 in Escherichia coli, substrate 4-nitrophenyl-beta-D-galactopyranoside
0.025
-
crude homogenate, at pH 4.8, 60°C
0.04
recombinant isozyme BGal2 in Escherichia coli, substrate 2-nitrophenyl-beta-D-galactopyranoside
0.058
recombinant isozyme BGal1 in Escherichia coli, substrate 2-nitrophenyl-beta-D-galactopyranoside
0.083
recombinant isozyme BGal2 in Escherichia coli, substrate 4-nitrophenyl-beta-D-galactopyranoside
0.085
-
crude extract, at 37°C
0.19
-
wild type enzyme, using 20 mM 4-nitrophenyl-beta-D-galactopyranoside as substrate, at 37°C
0.22
-
mutant enzyme R109K, using 2.65 mM 4-nitrophenyl-beta-D-galactopyranoside as substrate, at 37°C
0.27
-
after 11.1fold purification, at pH 4.8, 60°C
0.3346
-
40% of nitrogen source for yeast is supplemented as urea
0.35
-
cell extract, in 50 mM potassium phosphate buffer, pH 7.3, containing 100 mM NaCl and 100 mM beta-mercaptoethanol
0.47
-
mutant enzyme R109K, using 20 mM 4-nitrophenyl-beta-D-galactopyranoside as substrate, at 37°C
0.5
-
wild type enzyme, using 2.65 mM 4-nitrophenyl-beta-D-galactopyranoside as substrate, at 37°C
0.581
-
purified enzyme
0.6
-
mutant enzyme R109W, using 2.65 mM 4-nitrophenyl-beta-D-galactopyranoside as substrate, at 37°C
0.6597
-
nitrogen source for yeast is ammonium sulfate
0.72
-
partially purified enzyme, 37°C
0.9
Kerstingiella geocarpa
-
-
0.96
-
wild-type, pH 6.5, 40°C
1.148
-
37°C, 3 min, pH 6.6, 20% of nitrogen source for yeast is supplemented as urea
1.3
crude enzyme, in 200 mM potassium phosphate buffer, pH 6.8, for 15 min at 37°C
1.52
-
mutant enzyme R109V, using 2.65 mM 4-nitrophenyl-beta-D-galactopyranoside as substrate, at 37°C
1.79
-
mutant enzyme R109W, using 20 mM 4-nitrophenyl-beta-D-galactopyranoside as substrate, at 37°C
109
-
purified enzyme
1093
-
His-tagged enzyme from crude extract, at 30°C in 0.1 M sodium phosphate, pH 7.0, 10 mM KCl, 1 mM MgSO4, 5 mM mercaptoethanol
11.6
Caldariella acidophila
-
-
110.8
113
-
purified enzyme
116.4
-
purified enzyme
121
substrate: beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-beta-D-Glc, pH 4.5, 60°C
125.2
1410
-
substrate lactose, pH 3.5, 40°C
152.3
pH 7.5, 80°C, activity of recombinant mutant enzyme F441Y, substrate: 2-nitrophenyl beta-D-galactopyranoside
16
-
partially purified enzyme, 75°C
168.6
17.7
substrate: lactose, pH 4.5, 60°C
1709
-
purified commercial preparation
191.6
-
purified enzyme from mutant strain H26-10-7
2.6
-
after 7fold purification, in 50 mM potassium phosphate buffer, pH 7.3, containing 100 mM NaCl and 100 mM beta-mercaptoethanol
2.62
-
mutant FMNP01Gal-2, pH 6.5, 40°C
2.68
substrate: 4-nitrophenyl alpha-D-glucopyranoside, pH 6.0, 60°C
20.33
-
purified isozyme BGT II
20.4
purified recombinant His-tagged enzyme
21.7
-
crude extract, at pH 6.0 and 80°C
212
-
after 9.8fold purification, at pH 6.0 and 80°C
23.1
-
enzyme form 1
243
-
38°C, pH 7, substrate: 2-nitrophenyl-beta-D-galactopyranoside
2444
-
mature enzyme after 2.7fold purification, at 30°C in 0.1 M sodium phosphate, pH 7.0, 10 mM KCl, 1 mM MgSO4, 5 mM mercaptoethanol
25.4
-
38°C, pH 7, substrate: lactose
25.7
substrate: 4-nitrophenyl beta-D-glucopyranoside, pH 6.0, 60°C
25.76
-
beta-galactosidase V
27.2
substrate: 4-nitrophenyl beta-D-fucopyranoside, pH 6.0, 60°C
2969
-
His-tagged enzyme after 2.7fold purification, at 30°C in 0.1 M sodium phosphate, pH 7.0, 10 mM KCl, 1 mM MgSO4, 5 mM mercaptoethanol
299
substrate: 4-nitrophenyl beta-D-galactopyranoside, pH 4.5, 60°C
3 - 3.9
substrate: alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc, pH 4.5, 60°C
31.25
-
purified isozyme BGT I
33.25
-
beta-D-galactoside galactohydrolase pH 4.2
35.8
substrate: alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-[alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc, pH 4.5, 60°C
36.2
substrate: 4-nitrophenyl beta-D-galactopyranoside, pH 6.0, 60°C
39.79
-
beta-galactosidase I
4.15
substrate: 4-nitrophenyl beta-D-xylopyranoside, pH 6.0, 60°C
4.9
-
mutant enzyme R109V, using 20 mM 4-nitrophenyl-beta-D-galactopyranoside as substrate, at 37°C
40.2
-
purified enzyme from wild-type strain H26
42
substrate: 2-nitrophenyl-beta D-galactoside, pH 7.2, 22°C, the enzyme also shows beta-fucosidase activity
464
-
purified enzyme, substrate: 2-nitrophenyl beta-D-galactopyranoside, pH 6.5, 25-40°C
48.6
-
-
592
purified native enzyme
6.5
-
cell-free extract
62.83
-
-
65.1
-
-
69.3
after 53.3fold purification, in 200 mM potassium phosphate buffer, pH 6.8, for 15 min at 37°C
71
substrate: beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)-beta-D-Glc-(1->4)-[beta-D-Gal-(1->2)-alpha-D-Xyl-(1->6)]-beta-D-Glc-(1->4)-beta-D-Glc, pH 4.5, 60°C
77.33
-
after 910fold purification, at 37°C
77.5
-
enzyme form 2
82.4
-
enzyme form 3
898
-
mature enzyme from crude extract, at 30°C in 0.1 M sodium phosphate, pH 7.0, 10 mM KCl, 1 mM MgSO4, 5 mM mercaptoethanol
90
Saccharomyces fragilis
-
-
95.49
-
beta-galactosidase II
95.69
-
beta-galactosidase III
97
-
pH 6.5, 30°C, substrate: 2-nitrophenyl beta-D-galactopyranoside
additional information