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Literature summary for 3.2.1.23 extracted from

  • Sheridan, P.P.; Brenchley, J.E.
    Characterization of a salt-tolerant family 42 beta-galactosidase from a psychrophilic antarctic Planococcus isolate (2000), Appl. Environ. Microbiol., 66, 2438-2444.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
lactose 10 mM, 29% inhibition at 1.1 mM o-nitrophenyl beta-D-galactopyranoside Planococcus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Planococcus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
KCl still active in presence of 4 M, more active in presence of KCl than in presence of NaCl Planococcus sp. (in: Bacteria)
NaCl still active in presence of 4 M, more active in presence of KCl than in presence of NaCl Planococcus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
2 * 75000, SDS-PAGE Planococcus sp. (in: Bacteria)
155000
-
light scattering experiments Planococcus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Planococcus sp. (in: Bacteria) Q9KI47
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Planococcus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
o-nitrophenyl beta-D-fucopyranoside + H2O 6.1% of the activity with o-nitrophenyl beta-D-galactopyranoside Planococcus sp. (in: Bacteria) o-nitrophenol + beta-D-fucose
-
?
o-nitrophenyl beta-D-galactopyranoside + H2O
-
Planococcus sp. (in: Bacteria) o-nitrophenol + beta-D-galactose
-
?
p-nitrophenyl beta-D-fucopyranoside + H2O 3.8% of the activity with o-nitrophenyl beta-D-galactopyranoside Planococcus sp. (in: Bacteria) p-nitrophenol + beta-D-fucose
-
?
p-nitrophenyl beta-D-galactopyranoside + H2O 93% of the activity with o-nitrophenyl beta-D-galactopyranoside Planococcus sp. (in: Bacteria) p-nitrophenol + beta-D-galactose
-
?

Subunits

Subunits Comment Organism
dimer 2 * 75000, SDS-PAGE Planococcus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42
-
-
Planococcus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
24 56 24°C: about 45% of maximal activity, 56°C: about 70% of maximal activity Planococcus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
42
-
2 h, stable Planococcus sp. (in: Bacteria)
48
-
1 h, about 35% loss of activity Planococcus sp. (in: Bacteria)
55
-
10 min, complete and irreversible inactivation after 10 min Planococcus sp. (in: Bacteria)

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
81.8
-
o-nitrophenyl beta-D-galactopyranoside 1.9°C Planococcus sp. (in: Bacteria)
104
-
o-nitrophenyl beta-D-galactopyranoside 10°C Planococcus sp. (in: Bacteria)
288
-
o-nitrophenyl beta-D-galactopyranoside 20°C Planococcus sp. (in: Bacteria)
507
-
o-nitrophenyl beta-D-galactopyranoside 30°C Planococcus sp. (in: Bacteria)
603
-
o-nitrophenyl beta-D-galactopyranoside 39°C Planococcus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Planococcus sp. (in: Bacteria)