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Literature summary for 3.2.1.23 extracted from
- Characterization of Sulfolobus solfataricus beta-galactosidase mutant F441Y expressed in Pichia pastoris (2014), J. Sci. Food Agric., 94, 1359-1365.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the F441Y mutant enzyme has potential application in the industrial preparation of galactooligosaccharides | Saccharolobus solfataricus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| mutant F441Y is expressed in Pichia pastoris | Saccharolobus solfataricus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F441Y | mutant enzyme shows good thermostability | Saccharolobus solfataricus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 26.3 | - |
lactose | pH 7.5, 80°C, recombinant mutant enzyme F441Y | Saccharolobus solfataricus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
x * 56000, SDS-PAGE | Saccharolobus solfataricus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | P22498 | - |
- |
| Saccharolobus solfataricus P2 | P22498 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| mutant enzyme F441Y | Saccharolobus solfataricus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 152.3 | - |
pH 7.5, 80°C, activity of recombinant mutant enzyme F441Y, substrate: 2-nitrophenyl beta-D-galactopyranoside | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus | 2-nitrophenol + beta-D-galactose | - |
? | |
| 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Saccharolobus solfataricus P2 | 2-nitrophenol + beta-D-galactose | - |
? | |
| lactose + H2O | - |
Saccharolobus solfataricus | D-glucose + D-galactose | - |
? | |
| lactose + H2O | - |
Saccharolobus solfataricus P2 | D-glucose + D-galactose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 56000, SDS-PAGE | Saccharolobus solfataricus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LACS | - |
Saccharolobus solfataricus |
| SSO3019 | locus name | Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
recombinant mutant enzyme F441Y, substrate: 2-nitrophenyl beta-D-galactopyranoside | Saccharolobus solfataricus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | 95 | 65°C; about 55% of maximal activity, 95°C: about 90% of maximal activity, recombinant mutant enzyme F441Y, substrate: 2-nitrophenyl-beta-D-galactopyranoside | Saccharolobus solfataricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
50% loss of activity after 12 h, recombinant mutant enzyme F441Y | Saccharolobus solfataricus |
| 80 | - |
50% loss of activity after 11 h, recombinant mutant enzyme F441Y | Saccharolobus solfataricus |
| 85 | - |
50% loss of activity after 5.5 h, recombinant mutant enzyme F441Y | Saccharolobus solfataricus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.67 | - |
lactose | pH 7.5, 80°C, recombinant mutant enzyme F441Y | Saccharolobus solfataricus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
recombinant mutant enzyme F441Y, substrate: 2-nitrophenyl beta-D-galactopyranoside | Saccharolobus solfataricus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7.5 | pH 4.5: about 85% of maximal activity, pH 7.5: about 50% of maximal activity, recombinant mutant enzyme F441Y, substrate: 2-nitrophenyl-beta-D-galactopyranoside | Saccharolobus solfataricus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
half-life: 30 h | Saccharolobus solfataricus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
lactose | pH 7.5, 80°C, recombinant mutant enzyme F441Y | Saccharolobus solfataricus | |
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