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Literature summary for 3.2.1.23 extracted from

  • O'Connell, S.; Walsh, G.
    Purification and properties of a beta-galactosidase with potential application as a digestive supplement (2007), Appl. Biochem. Biotechnol., 141, 1-14.
    View publication on PubMed
Search for more literature for 3.2.1.23

Application

Application Comment Organism
medicine use of enzyme as a digestive supplement Kluyveromyces marxianus
nutrition the enzyme has a potential application as a digestive supplement Kluyveromyces marxianus
nutrition use of enzyme as a digestive supplement Kluyveromyces marxianus

General Stability

General Stability Organism
the unpurified beta-galactosidase displays a high level of stability when exposed to simulated intestinal conditions in vitro for 4 h Kluyveromyces marxianus
the unpurified enzyme displays a high level of stability when exposed to simulated intestinal conditions in vitro for 4 h Kluyveromyces marxianus

Inhibitors

Inhibitors Comment Organism Structure
D-galactose 5 mM, 93% residual activity Kluyveromyces marxianus
D-glucose 5 mM, 86% residual activity Kluyveromyces marxianus
isopropyl beta-D-thiogalactoside 5 mM, 91% residual activity Kluyveromyces marxianus
additional information no or poor inhibition by D-glucose, D-galactose, IPTG, and sucrose; no or poor inhibition by D-glucose, D-galactose, IPTG, and sucrose Kluyveromyces marxianus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4
-
 lactose pH 6.8 Kluyveromyces marxianus
4
-
 lactose pH 6.8, 37°C Kluyveromyces marxianus
4.1
-
 2-nitrophenyl-beta-D-galactopyranoside pH 6.8, 37°C Kluyveromyces marxianus
4.7
-
 2-nitrophenyl-beta-D-galactoside pH 6.8 Kluyveromyces marxianus
7.1
-
 4-nitrophenyl-beta-D-galactoside pH 6.8 Kluyveromyces marxianus
7.1
-
 4-nitrophenyl-beta-D-galactopyranoside pH 6.8, 37°C Kluyveromyces marxianus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
123000
-
 2 * 123000, SDS-PAGE Kluyveromyces marxianus
251000
-
 gel filtration Kluyveromyces marxianus
251000
-
 PAGE Kluyveromyces marxianus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lactose + H2O Kluyveromyces marxianus the enzyme is involved in lactose hydrolysis in the small intestine of the host organism D-glucose + D-galactose
-
 ?

Organism

Organism UniProt Comment Textmining
Kluyveromyces marxianus
-
 strain DSM5418
-
Kluyveromyces marxianus P00723 strain DSM 5418
-
Kluyveromyces marxianus Q6QTF4 strain DSM 5418
-
Kluyveromyces marxianus DSM 5418
-
 strain DSM5418
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein
-
 Kluyveromyces marxianus
proteolytic modification pancreatin activates the beta-galactosidase due to a partial proteolytic cleavage yielding a more active enzyme form Kluyveromyces marxianus

Purification (Commentary)

Purification (Comment) Organism
-
 Kluyveromyces marxianus
native enzyme 115fold to homogeneity by gel filtration, and anion exchange and hydroxylapatite chromatography Kluyveromyces marxianus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Kluyveromyces marxianus
790
-
 pH 6.8 Kluyveromyces marxianus
790.6
-
 purified enzyme Kluyveromyces marxianus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Kluyveromyces marxianus 2-nitrophenol + beta-D-galactose
-
 ?
2-nitrophenyl beta-D-galactoside + H2O
-
 Kluyveromyces marxianus 2-nitrophenol + D-galactose
-
 ?
2-nitrophenyl beta-D-galactoside + H2O
-
 Kluyveromyces marxianus DSM 5418 2-nitrophenol + D-galactose
-
 ?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Kluyveromyces marxianus 4-nitrophenol + beta-D-galactose
-
 ?
4-nitrophenyl beta-D-galactoside + H2O
-
 Kluyveromyces marxianus 4-nitrophenol + D-galactose
-
 ?
4-nitrophenyl beta-D-galactoside + H2O
-
 Kluyveromyces marxianus DSM 5418 4-nitrophenol + D-galactose
-
 ?
lactose + H2O
-
 Kluyveromyces marxianus D-glucose + D-galactose
-
 ?
lactose + H2O the enzyme is involved in lactose hydrolysis in the small intestine of the host organism Kluyveromyces marxianus D-glucose + D-galactose
-
 ?
lactose + H2O
-
 Kluyveromyces marxianus D-galactose + D-glucose
-
 ?
lactose + H2O
-
 Kluyveromyces marxianus DSM 5418 D-galactose + D-glucose
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 123000, SDS-PAGE Kluyveromyces marxianus
More peptide mass fingerprinting Kluyveromyces marxianus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Kluyveromyces marxianus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
22 50 temperature profile, about 50% of maximal activity between 20°C and 45°C Kluyveromyces marxianus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6300
-
 lactose pH 6.8 Kluyveromyces marxianus
6300
-
 lactose pH 6.8, 37°C Kluyveromyces marxianus
8559
-
 2-nitrophenyl-beta-D-galactoside pH 6.8 Kluyveromyces marxianus
8559
-
 2-nitrophenyl-beta-D-galactopyranoside pH 6.8, 37°C Kluyveromyces marxianus
270000
-
 4-nitrophenyl-beta-D-galactopyranoside pH 6.8, 37°C Kluyveromyces marxianus
2700000
-
 4-nitrophenyl-beta-D-galactoside pH 6.8 Kluyveromyces marxianus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
 Kluyveromyces marxianus

pH Range

pH Minimum pH Maximum Comment Organism
3.5 10 about 50% of maximal activity between pH 5.6 and pH 7.8 Kluyveromyces marxianus

pI Value

Organism Comment pI Value Maximum pI Value
Kluyveromyces marxianus isoelectric focusing
-
 5.1