Any feedback?
Literature summary for 3.2.1.23 extracted from
- Dissociation and catalytic activity of oligomer forms of beta-galactosidases (2007), Russ. J. Phys. Chem. A, 81, 990-994.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Escherichia coli | |
| additional information | - |
additional information | kinetics | Bos taurus | |
| additional information | - |
additional information | kinetics | Kluyveromyces marxianus | |
| additional information | - |
additional information | kinetics | Penicillium canescens | |
| 0.093 | - |
2-nitrophenyl-beta-D-galactopyranoside | tetrameric enzyme, 25°C | Escherichia coli |  |
| 1 | - |
2-nitrophenyl-beta-D-galactopyranoside | dimeric enzyme, 25°C | Kluyveromyces marxianus | |
| 1 | - |
2-nitrophenyl-beta-D-galactopyranoside | tetrameric enzyme, 25°C | Penicillium canescens | |
| 2.5 | - |
2-nitrophenyl-beta-D-galactopyranoside | 25°C | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 12000 | - |
1 * 90000 + 1 * 12000 | Kluyveromyces marxianus |
| 41000 | - |
1 * 41000 | Bos taurus |
| 90000 | - |
1 * 90000 + 1 * 12000 | Kluyveromyces marxianus |
| 116000 | - |
4 * 116000 | Escherichia coli |
| 116000 | - |
2 * 116000 | Escherichia coli |
| 203000 | - |
dimeric enzyme form | Kluyveromyces marxianus |
| 220000 | 280000 | dimeric enzyme form | Penicillium canescens |
| 232000 | - |
dimeric enzyme form | Escherichia coli |
| 440000 | 560000 | tetrameric enzyme form | Penicillium canescens |
| 464000 | - |
tetrameric enzyme form | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Kluyveromyces marxianus | - |
- |
- |
| Penicillium canescens | - |
- |
- |
| Penicillium canescens F-178 | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Escherichia coli | - |
| commercial preparation | - |
Bos taurus | - |
| commercial preparation | - |
Kluyveromyces marxianus | - |
| liver | commercial preparation | Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
| additional information | - |
- |
Bos taurus |
| additional information | - |
- |
Kluyveromyces marxianus |
| additional information | - |
- |
Penicillium canescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Escherichia coli | 2-nitrophenol + beta-D-galactose | - |
? | |
| 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Bos taurus | 2-nitrophenol + beta-D-galactose | - |
? | |
| 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Kluyveromyces marxianus | 2-nitrophenol + beta-D-galactose | - |
? | |
| 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Penicillium canescens | 2-nitrophenol + beta-D-galactose | - |
? | |
| 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Penicillium canescens F-178 | 2-nitrophenol + beta-D-galactose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 90000 + 1 * 12000 | Kluyveromyces marxianus |
| dimer | 2 * 11000-140000 | Penicillium canescens |
| dimer | 2 * 116000 | Escherichia coli |
| monomer | 1 * 41000 | Bos taurus |
| More | other oligomer forms of the enzyme are inactive | Escherichia coli |
| More | other oligomer forms of the enzyme are inactive | Kluyveromyces marxianus |
| More | other oligomer forms of the enzyme are inactive | Penicillium canescens |
| tetramer | 4 * 116000 | Escherichia coli |
| tetramer | 4 * 11000-140000 | Penicillium canescens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Escherichia coli |
| 25 | - |
assay at | Bos taurus |
| 25 | - |
assay at | Kluyveromyces marxianus |
| 25 | - |
assay at | Penicillium canescens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 45 | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | 0.08 | 2-nitrophenyl-beta-D-galactopyranoside | 25°C | Bos taurus | |
| 45.4 | - |
2-nitrophenyl-beta-D-galactopyranoside | dimeric enzyme, 25°C | Kluyveromyces marxianus | |
| 45.4 | - |
2-nitrophenyl-beta-D-galactopyranoside | dimeric enzyme, 25°C | Penicillium canescens | |
| 180 | - |
2-nitrophenyl-beta-D-galactopyranoside | dimeric enzyme, 25°C | Escherichia coli | |
| 400 | - |
2-nitrophenyl-beta-D-galactopyranoside | tetrameric enzyme, 25°C | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.2 | 4.5 | - |
Penicillium canescens |
| 7 | 7.5 | - |
Escherichia coli |
| 7 | 7.5 | - |
Bos taurus |
| 7.2 | 7.5 | - |
Kluyveromyces marxianus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
