1.3.5.2: dihydroorotate dehydrogenase (quinone)

This is an abbreviated version!
For detailed information about dihydroorotate dehydrogenase (quinone), go to the full flat file.

Word Map on EC 1.3.5.2

Reaction

(S)-dihydroorotate
+
a quinone
=
Orotate
+
a quinol

Synonyms

class 2 dihydroorotate dehydrogenases, DHO-DH, DHOD, DHODase, DHODH, HsDHODH, L-5,6-dihydroorotate:ubiquinone exidoreductase, PfDHODH

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.5 With a quinone or related compound as acceptor
                1.3.5.2 dihydroorotate dehydrogenase (quinone)

Cloned

Cloned on EC 1.3.5.2 - dihydroorotate dehydrogenase (quinone)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
; expressed in Escherichia coli DH5alpha TAP330, lacking the endogenous gene for the bacterial dihydroorotate dehydrogenase, pyrimidine auxotroph
-
; expression in Escherichia coli BL21(DE3) TAP330, a pyrD(-) minus derivative
-
expressed in Escherichia coli BL21 (DE3) cells
-
expressed in Escherichia coli Bl21-CodonPlus (DE3)-RP cells
-
expressed in Escherichia coli DH5alpha TAP330, lacking the endogenous gene for the bacterial dihydroorotate dehydrogenase, pyrimidine auxotroph
-
expressed in Escherichia coli XL-1 Blue, histidine tagged
expressed in Escherichia coli XL-1 Blue, histidine tagged; expression of complete catalytically active enzyme in SF21 insect cells of Spodoptera frugiperda with baculovirus vector system
-
expressed in pyr4 mutants of Ustilago maydis
-
expression in Escherichia coli
-
expression in Escherichia coli pyrD using a construct with His-tag fusion
expression of GFP-tagged enzyme in SW480 cells
-
expression of the expression construct pET28b-TEVpfDHODDELTA384–413 in Escherichia coli strain BL21
-
full-length DHODH and N-terminally truncated DHODH, which lacks the targeting sequence and the transmembrane domain recombinantly expressed in Escherichia coli BL21
overexpression of Escherichia coli dihyroorotate dehyrogenase in same strain, partially deleted for the chromosomal pyrD gene, clone selection followed by ampicillin and by complementation of the pyrimidine requirement
-