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Literature summary for 1.3.5.2 extracted from

  • Garavito, M.F.; Narvaez-Ortiz, H.Y.; Pulido, D.C.; Loeffler, M.; Judelson, H.S.; Restrepo, S.; Zimmermann, B.H.
    Phytophthora infestans dihydroorotate dehydrogenase is a potential target for chemical control - a comparison with the enzyme from Solanum tuberosum (2019), Front. Microbiol., 10, 1479 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.5.2

Activating Compound

Activating Compound Comment Organism Structure
2-(4-biphenylylcarbamoyl)-2-benzoic acid i.e. MD 129, 0.5 mM, 103% of initial activity Phytophthora infestans
alloxan 0.5 mM, 112% of initial activity Phytophthora infestans
atovaquone 0.5 mM, 127% of initial activity Phytophthora infestans
NSC 277965 0.5 mM, 130% of initial activity Solanum tuberosum
NSC 71097 0.5 mM, 129% of initial activity Phytophthora infestans

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Phytophthora infestans
expression in Escherichia coli Solanum tuberosum

Protein Variants

Protein Variants Comment Organism
additional information expression of N-terminally truncated versions lacking the transmembrane anchor Phytophthora infestans
additional information expression of N-terminally truncated versions lacking the transmembrane anchor Solanum tuberosum

Inhibitors

Inhibitors Comment Organism Structure
2-(4-biphenylylcarbamoyl)-1-cyclopentene-1-carboxylic acid i.e. MD 108, 0.5 mM, 23% of initial activity Phytophthora infestans
2-(4-biphenylylcarbamoyl)-1-cyclopentene-1-carboxylic acid i.e. MD 108, 0.5 mM, 90% of initial activity Solanum tuberosum
2-(4-biphenylylcarbamoyl)-2-benzoic acid i.e. MD 129, 0.5 mM, 95% of initial activity Solanum tuberosum
A77-1726 0.5 mM, 14% of initial activity, inhibits the growth of Phytophthora infestans Phytophthora infestans
A77-1726 0.5 mM, 97% of initial activity Solanum tuberosum
alloxan 0.5 mM, 74% of initial activity Solanum tuberosum
ametoctradin 0.5 mM, 41% of initial activity Phytophthora infestans
ametoctradin 0.5 mM,102% of initial activity Solanum tuberosum
atovaquone 0.5 mM, 127% of initial activity Phytophthora infestans
atovaquone 0.5 mM, 75% of initial activity Solanum tuberosum
brequinar 0.5 mM, 98% of initial activity Phytophthora infestans
brequinar 0.5 mM, 49% of initial activity Solanum tuberosum
dichloroallyl lawsone 0.5 mM, 30% of initial activity Phytophthora infestans
dichloroallyl lawsone 0.5 mM, 39% of initial activity Solanum tuberosum
N-(3-chloro-2-methoxybiphenyl-4-yl)-2-cyano-3-hydroxybut-2-enamide i.e. MD 209, 0.5 mM, 2% of initial activity Phytophthora infestans
N-(3-chloro-2-methoxybiphenyl-4-yl)-2-cyano-3-hydroxybut-2-enamide i.e. MD 209, 0.5 mM, 0% of initial activity Solanum tuberosum
N-(3-chlorobiphenyl-4-yl)-2-cyano-3-hydroxybut-2-enamide i.e. MD 241, 0.5 mM, 1% of initial activity Phytophthora infestans
N-(3-chlorobiphenyl-4-yl)-2-cyano-3-hydroxybut-2-enamide i.e. MD 241, 0.5 mM, 87% of initial activity Solanum tuberosum
N-[3,5-difluoro-4-(trifluoromethyl)phenyl]-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine i.e. DSM190, 0.5 mM, 36% of initial activity Phytophthora infestans
N-[3,5-difluoro-4-(trifluoromethyl)phenyl]-5-methyl[1,2,4]triazolo[1,5-a]pyrimidin-7-amine i.e. DSM190, 0.5 mM, 38% of initial activity Solanum tuberosum
NSC 277965 0.5 mM, 60% of initial activity Phytophthora infestans
NSC 61890 0.5 mM, 93% of initial activity Phytophthora infestans
NSC 61890 0.5 mM, 36% of initial activity Solanum tuberosum
NSC 71097 0.5 mM, 70% of initial activity Solanum tuberosum
redoxal 0.5 mM, 31% of initial activity Phytophthora infestans
redoxal 0.5 mM, 39% of initial activity Solanum tuberosum
toltrazuril 1 mM, 67% of initial activity Phytophthora infestans
toltrazuril 1 mM, 0% of initial activity Solanum tuberosum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 coenzyme QD pH 8, 30°C Phytophthora infestans
0.02
-
 (S)-dihydroorotate pH 8, 30°C Solanum tuberosum
0.03
-
 coenzyme QD pH 8, 30°C Solanum tuberosum
0.057
-
 (S)-dihydroorotate pH 8, 30°C Phytophthora infestans

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Phytophthora infestans 5739
-
mitochondrion
-
 Solanum tuberosum 5739
-

Organism

Organism UniProt Comment Textmining
Phytophthora infestans I7EMP0
-
-
Solanum tuberosum M1BCR0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-dihydroorotate + 1,4-naphthoquinone 50% of the activity with coenzyme Qd Phytophthora infestans orotate + 1,4-naphthoquinol
-
 ?
(S)-dihydroorotate + 1,4-naphthoquinone 78% of the activity with coenzyme Qd Solanum tuberosum orotate + 1,4-naphthoquinol
-
 ?
(S)-dihydroorotate + 2,5-dimethyl-p-benzoquinone 48% of the activity with coenzyme Qd Phytophthora infestans orotate + 2,5-dimethyl-p-benzoquinol
-
 ?
(S)-dihydroorotate + 2,5-dimethyl-p-benzoquinone 67% of the activity with coenzyme Qd Solanum tuberosum orotate + 2,5-dimethyl-p-benzoquinol
-
 ?
(S)-dihydroorotate + 2,6-dihydrophenolindophenol
-
 Phytophthora infestans orotate + reduced 2,6-dihydrophenolindophenol
-
 ?
(S)-dihydroorotate + 2,6-dihydrophenolindophenol
-
 Solanum tuberosum orotate + reduced 2,6-dihydrophenolindophenol
-
 ?
(S)-dihydroorotate + 5,8-dihydroxy-1,4-naphthoquinone 78% of the activity with coenzyme Qd Solanum tuberosum orotate + 5,8-dihydroxy-1,4-naphthoquinol
-
 ?
(S)-dihydroorotate + coenzyme Q0 56% of the activity with coenzyme Qd Phytophthora infestans orotate + reduced coenzyme Q0
-
 ?
(S)-dihydroorotate + coenzyme Q0 96% of the activity with coenzyme Qd Solanum tuberosum orotate + reduced coenzyme Q0
-
 ?
(S)-dihydroorotate + coenzyme Q1 111% of the activity with coenzyme Qd Phytophthora infestans orotate + reduced coenzyme Q1
-
 ?
(S)-dihydroorotate + coenzyme Q1 124% of the activity with coenzyme Qd Solanum tuberosum orotate + reduced coenzyme Q1
-
 ?
(S)-dihydroorotate + coenzyme Q10 19% of the activity with coenzyme Qd Solanum tuberosum orotate + reduced coenzyme Q10
-
 ?
(S)-dihydroorotate + coenzyme Q10 30% of the activity with coenzyme Qd Phytophthora infestans orotate + reduced coenzyme Q10
-
 ?
(S)-dihydroorotate + coenzyme Q2 123% of the activity with coenzyme Qd Solanum tuberosum orotate + reduced coenzyme Q2
-
 ?
(S)-dihydroorotate + coenzyme Q6 102% of the activity with coenzyme Qd Solanum tuberosum orotate + reduced coenzyme Q6
-
 ?
(S)-dihydroorotate + coenzyme Q6 144% of the activity with coenzyme Qd Phytophthora infestans orotate + reduced coenzyme Q6
-
 ?
(S)-dihydroorotate + coenzyme Q9 111% of the activity with coenzyme Qd Solanum tuberosum orotate + reduced coenzyme Q9
-
 ?
(S)-dihydroorotate + coenzyme Q9 66% of the activity with coenzyme Qd Phytophthora infestans orotate + reduced coenzyme Q9
-
 ?
(S)-dihydroorotate + coenzyme Qd
-
 Phytophthora infestans orotate + reduced coenzyme Qd
-
 ?
(S)-dihydroorotate + coenzyme Qd
-
 Solanum tuberosum orotate + reduced coenzyme Qd
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
30
-
 (S)-dihydroorotate pH 8, 30°C Solanum tuberosum
75
-
 (S)-dihydroorotate pH 8, 30°C Phytophthora infestans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1000
-
 coenzyme QD pH 8, 30°C Solanum tuberosum
1300
-
 (S)-dihydroorotate pH 8, 30°C Phytophthora infestans
1500
-
 (S)-dihydroorotate pH 8, 30°C Solanum tuberosum
4900
-
 coenzyme QD pH 8, 30°C Phytophthora infestans