Activating Compound | Comment | Organism | Structure |
---|---|---|---|
oxygen | seems to promote very low DHODH activity | Candida albicans |
Application | Comment | Organism |
---|---|---|
medicine | background for the development of antipyrimidines with high efficacy for decreasing in situ pyrimidine nucleotide pools in Candida albicans | Candida albicans |
Cloned (Comment) | Organism |
---|---|
full-length DHODH and N-terminally truncated DHODH, which lacks the targeting sequence and the transmembrane domain recombinantly expressed in Escherichia coli BL21 | Candida albicans |
Protein Variants | Comment | Organism |
---|---|---|
L11S | two CUG codons in the DHODH ORF changed to UCG by site-directed PCR mutagenesis for gene expression in the bacterial system | Candida albicans |
L78S | two CUG codons in the DHODH ORF changed to UCG by site-directed PCR mutagenesis for gene expression in the bacterial system | Candida albicans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
brequinar sodium | 1 mM reduces the activity by more than 50% of the full-length enzyme | Candida albicans | |
redoxal | 0.5 mM exhibit an inhibitory effect of more than 50% on full-length DHODH and N-terminally truncated DHODH compared with the noninhibited reaction | Candida albicans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
dihydroorotate | N-terminally truncated DHODH, fixed concentration of dihydroorotate and varied concentrations of 2,6-dichloroindophenol from 0-0.2 mM | Candida albicans | |
0.042 | - |
dihydroorotate | full-length DHODH, fixed concentration of dihydroorotate of 1.0 mM and varied concentrations of decylubiquinone from 0-0.2 mM | Candida albicans | |
0.065 | - |
dihydroorotate | N-terminally truncated DHODH, fixed concentration of dihydroorotate of 1.0 mM and varied concentrations of decylubiquinone from 0-0.2 mM | Candida albicans | |
0.108 | - |
dihydroorotate | full-length DHODH, concentration of dihydroorotate varied from 0-1.0 mM at fixed concentrations of 0.1 mM decylubiquinone and 0.06 mM 2,6-dichloroindophenol | Candida albicans | |
0.111 | - |
dihydroorotate | N-terminally truncated DHODH, concentration of dihydroorotate varied from 0-1.0 mM at fixed concentrations of 0.1 mM decylubiquinone and 0.06 mM 2,6-dichloroindophenol | Candida albicans | |
0.122 | - |
dihydroorotate | full-length DHODH, fixed concentration of dihydroorotate of 1.0 mM and varied concentrations of 2,6-dichloroindophenol from 0-0.2 mM | Candida albicans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
48000 for the full-length enzyme and 42000 for the truncated enzyme, SDS-PAGE | Candida albicans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | Q874I4 | - |
- |
Purification (Comment) | Organism |
---|---|
full-length DHODH and N-terminally truncated DHODH, by affinity chromatography | Candida albicans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6 | - |
using decylubiquinone and 2,6-dichloroindophenol as acceptor | Candida albicans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroorotate + 2,3-dimethoxy-5-methyl-1,4-benzoquinone | - |
Candida albicans | orotate + reduced 2,3-dimethoxy-5-methyl-1,4-benzoquinone | - |
? | |
dihydroorotate + 2,6-dichloroindophenol | - |
Candida albicans | orotate + reduced 2,6-dichloroindophenol | - |
? | |
dihydroorotate + decylubiquinone | - |
Candida albicans | orotate + reduced decylubiquinone | - |
? | |
dihydroorotate + potassium hexacyanoferrate(III) | - |
Candida albicans | orotate + reduced potassium hexacyanoferrate(III) | - |
? | |
dihydroorotate + ubiquinone 30 | - |
Candida albicans | orotate + reduced ubiquinone 30 | - |
? | |
dihydroorotate + ubiquinone 50 | - |
Candida albicans | orotate + reduced ubiquinone 50 | - |
? | |
additional information | fumarate and NAD are inadequate electron acceptors for full-length DHODH and N-terminally truncated DHODH | Candida albicans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | 48000 for the full-length enzyme and 42000 for the truncated enzyme, SDS-PAGE | Candida albicans |
Synonyms | Comment | Organism |
---|---|---|
DHODH | - |
Candida albicans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.1 | - |
dihydroorotate | full-length DHODH | Candida albicans | |
2.2 | - |
dihydroorotate | N-terminally truncated DHODH | Candida albicans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
8.0-8.5 | Candida albicans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | - |
Candida albicans |