1.3.5.2: dihydroorotate dehydrogenase (quinone)

This is an abbreviated version!
For detailed information about dihydroorotate dehydrogenase (quinone), go to the full flat file.

Word Map on EC 1.3.5.2

Reaction

(S)-dihydroorotate
+
a quinone
=
Orotate
+
a quinol

Synonyms

class 2 dihydroorotate dehydrogenases, DHO-DH, DHOD, DHODase, DHODH, HsDHODH, L-5,6-dihydroorotate:ubiquinone exidoreductase, PfDHODH

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.5 With a quinone or related compound as acceptor
                1.3.5.2 dihydroorotate dehydrogenase (quinone)

Purification

Purification on EC 1.3.5.2 - dihydroorotate dehydrogenase (quinone)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
; cation-, anion-exchange, gel filtration
-
; ion-exchange, hydroxyapatite, gel filtration for U937, cation-exchange for spleen
-
ammonium sulfate precipitation, affinity chromatography, ion-exchange chromatography
-
by nickel agarose chromatography
full-length DHODH and N-terminally truncated DHODH, by affinity chromatography
ion-exchange, ammonium sulfate precipitation, gel filtration
-
Ni-NTA column chromatography
-
Ni2+/nitrilotriacetate column
recombinant His-tagged mutant DHODDELTA384-413 from Escherichia coli by affinity chromatography and gel filtration
-
recombinant protein, His8-tag
-
streptomycin sulfate precipitation, anion-exchange, gel filtration
-
Talon resin column chromatography
-