Literature summary for 1.3.5.2 extracted from

Copeland, R.A.; Davis, J.P.; Dowling, R.L.; Lombardo, D.; Murphy, K.B.; Patterson, T.A.
Recombinant human dihydroorotate dehydrogenase: expression, purification, and characterization of a catalytically functional truncated enzyme (1995), Arch. Biochem. Biophys., 323, 79-86.

Search for more literature for 1.3.5.2

Application

Application	Comment	Organism
medicine	great interest in inhibitors as potential therapeutic agents for the treatment of diseases involving aberrant cell proliferation	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
-	Homo sapiens
expressed in Escherichia coli DH5alpha TAP330, lacking the endogenous gene for the bacterial dihydroorotate dehydrogenase, pyrimidine auxotroph	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
brequinar sodium	potent, noncompetitive inhibitor	Homo sapiens
diethyldicarbonate	histidine-selective covalent modifier; histidine-selective modifying agent, dose-dependent	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0155	-	S-dihydroorotate	-	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Homo sapiens	16020	-
mitochondrion	inner membrane	Homo sapiens	5739	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	x * 40000, recombinant enzyme, SDS-PAGE, x * 50000, native full-length enzyme, SDS-PAGE	Homo sapiens
40000	-	? * 40000	Homo sapiens
50000	-	x * 40000, recombinant enzyme, SDS-PAGE, x * 50000, native full-length enzyme, SDS-PAGE	Homo sapiens
120000	-	gel filtration	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(S)-dihydroorotate + ubiquinone	Homo sapiens	fourth step in synthesis of uridine 5'-monophosphate	orotate + ubiquinol	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-
Homo sapiens	-	truncated recombinant protein	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, affinity chromatography, ion-exchange chromatography	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.49	-	-	Homo sapiens

Storage Stability

Storage Stability	Organism
-70°C, flash frozen	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-dihydroorotate + ubiquinone	-	Homo sapiens	orotate + ubiquinol	-	?
(S)-dihydroorotate + ubiquinone	fourth step in synthesis of uridine 5'-monophosphate	Homo sapiens	orotate + ubiquinol	-	?

Subunits

Subunits	Comment	Organism
?	x * 40000, recombinant enzyme, SDS-PAGE, x * 50000, native full-length enzyme, SDS-PAGE	Homo sapiens
?	? * 40000	Homo sapiens

Synonyms

Synonyms	Comment	Organism
DHODase	-	Homo sapiens
L-5,6-dihydroorotate:ubiquinone exidoreductase	-	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	full-length native enzyme	Homo sapiens
8.5	-	recombinant enzyme	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
additional information	does not contain a flavin cofactor	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0000232	-	brequinar sodium	full-length native enzyme	Homo sapiens
0.0000265	-	brequinar sodium	recombinant enzyme	Homo sapiens

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Release 2023.1 (January 2023)