Application | Comment | Organism |
---|---|---|
medicine | great interest in inhibitors as potential therapeutic agents for the treatment of diseases involving aberrant cell proliferation | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
expressed in Escherichia coli DH5alpha TAP330, lacking the endogenous gene for the bacterial dihydroorotate dehydrogenase, pyrimidine auxotroph | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
brequinar sodium | potent, noncompetitive inhibitor | Homo sapiens | |
diethyldicarbonate | histidine-selective covalent modifier; histidine-selective modifying agent, dose-dependent | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0155 | - |
S-dihydroorotate | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
mitochondrion | inner membrane | Homo sapiens | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, recombinant enzyme, SDS-PAGE, x * 50000, native full-length enzyme, SDS-PAGE | Homo sapiens |
40000 | - |
? * 40000 | Homo sapiens |
50000 | - |
x * 40000, recombinant enzyme, SDS-PAGE, x * 50000, native full-length enzyme, SDS-PAGE | Homo sapiens |
120000 | - |
gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-dihydroorotate + ubiquinone | Homo sapiens | fourth step in synthesis of uridine 5'-monophosphate | orotate + ubiquinol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Homo sapiens | - |
truncated recombinant protein | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, affinity chromatography, ion-exchange chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.49 | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-70°C, flash frozen | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-dihydroorotate + ubiquinone | - |
Homo sapiens | orotate + ubiquinol | - |
? | |
(S)-dihydroorotate + ubiquinone | fourth step in synthesis of uridine 5'-monophosphate | Homo sapiens | orotate + ubiquinol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, recombinant enzyme, SDS-PAGE, x * 50000, native full-length enzyme, SDS-PAGE | Homo sapiens |
? | ? * 40000 | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
DHODase | - |
Homo sapiens |
L-5,6-dihydroorotate:ubiquinone exidoreductase | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
full-length native enzyme | Homo sapiens |
8.5 | - |
recombinant enzyme | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | does not contain a flavin cofactor | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000232 | - |
brequinar sodium | full-length native enzyme | Homo sapiens | |
0.0000265 | - |
brequinar sodium | recombinant enzyme | Homo sapiens |