1.11.1.12: phospholipid-hydroperoxide glutathione peroxidase

This is an abbreviated version!
For detailed information about phospholipid-hydroperoxide glutathione peroxidase, go to the full flat file.

Word Map on EC 1.11.1.12

Reaction

2 glutathione +

a hydroperoxy-fatty-acyl-[lipid]
=
glutathione disulfide
+
a hydroxy-fatty-acyl-[lipid]
+
H2O

Synonyms

AC-PHGPx, Bi-PHGPx, class 4 GPx, CsGPx1, CsGPx2, CsGPx3, CsGPx4, DMPHGPx, glutathione peroxidase 4, glutathione peroxidase-4, glutathione-dependent phospholipid peroxidase, GPX, GPx-4, GPX3, GPx4, GPx41, GPx4A, GPx4B, GPXhs2, GPXle1, hydroperoxide glutathione peroxidase, Hyr1, Hyr1/YIR037W, McPHGPx, More, non-selenocysteine PHGPx, NPGPx, nPHGPx, OsPHGPx, peroxidation-inhibiting protein, peroxidation-inhibiting protein: peroxidase, glutathione (phospholipid hydroperoxide-reducing), PHGPX, phospholipid glutathione peroxidase, phospholipid hydroperoxide glutathione peroxidase, phospholipid hydroperoxide glutathione peroxidase A, phospholipid hydroperoxide glutathione peroxidase B, phospholipid hydroperoxide glutathione peroxidase-4, phospholipid-hydroperoxide glutathione peroxidase, phospholipid-hydroperoxide glutathione peroxidases, selenium-dependent glutathione peroxidase type-4, seleno GPx, selenoperoxidase, selenoprotein P, sperm nucleus-specific glutathione peroxidase, type-4 glutathione peroxidase, VcPHGPx

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.12 phospholipid-hydroperoxide glutathione peroxidase

Purification

Purification on EC 1.11.1.12 - phospholipid-hydroperoxide glutathione peroxidase

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PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, Sephadex G-50 gel filtration, and HiTrap SP column chromatography
-
by Ni2+-nitrilotriacetic acid Sepharose
by nickel affinity chromatography
from cytosol and mitochondria
-
large-scale, wild-type and mutant SeC46C from baculovirus/insect cell expression system and mutant SeC46C from expression in Escherichia coli as His-tagged fusion protein
-
on a Ni-NTA column, further purified by gel filtration
-
partially
partially from testis and to homogenity from sperm as recombinant enzyme expressed in Escherichia coli and as wild-type enzyme
-
Percoll step gradient and nickel affinity chromatography
-
recombinant enzyme
-
recombinant enzyme from Escherichia coli
-
recombinant protein
Sec43Cys is purified on a GSH-Sepharose column, Sec43Ser is purified by ionic exchange chromatography, the GST-tags are cleaved with bovine thrombin
-
using a HisTrap column
wild-type and mutant proteins
-