1.11.1.12: phospholipid-hydroperoxide glutathione peroxidase

This is an abbreviated version!
For detailed information about phospholipid-hydroperoxide glutathione peroxidase, go to the full flat file.

Word Map on EC 1.11.1.12

Reaction

2 glutathione +

a hydroperoxy-fatty-acyl-[lipid]
=
glutathione disulfide
+
a hydroxy-fatty-acyl-[lipid]
+
H2O

Synonyms

AC-PHGPx, Bi-PHGPx, class 4 GPx, CsGPx1, CsGPx2, CsGPx3, CsGPx4, DMPHGPx, glutathione peroxidase 4, glutathione peroxidase-4, glutathione-dependent phospholipid peroxidase, GPX, GPx-4, GPX3, GPx4, GPx41, GPx4A, GPx4B, GPXhs2, GPXle1, hydroperoxide glutathione peroxidase, Hyr1, Hyr1/YIR037W, McPHGPx, More, non-selenocysteine PHGPx, NPGPx, nPHGPx, OsPHGPx, peroxidation-inhibiting protein, peroxidation-inhibiting protein: peroxidase, glutathione (phospholipid hydroperoxide-reducing), PHGPX, phospholipid glutathione peroxidase, phospholipid hydroperoxide glutathione peroxidase, phospholipid hydroperoxide glutathione peroxidase A, phospholipid hydroperoxide glutathione peroxidase B, phospholipid hydroperoxide glutathione peroxidase-4, phospholipid-hydroperoxide glutathione peroxidase, phospholipid-hydroperoxide glutathione peroxidases, selenium-dependent glutathione peroxidase type-4, seleno GPx, selenoperoxidase, selenoprotein P, sperm nucleus-specific glutathione peroxidase, type-4 glutathione peroxidase, VcPHGPx

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.12 phospholipid-hydroperoxide glutathione peroxidase

Engineering

Engineering on EC 1.11.1.12 - phospholipid-hydroperoxide glutathione peroxidase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C2S/C10S/C37S/C66S/C75S/C107S/C148S
-
substitution of all Cys residues by Ser. The mutant exhibits a Ping-Pong mechanism and structure similar to that of native GPx4, the activity is similar to wild-type
SeC46C
-
expression of mutant in Escherichia coli as His-tagged fusion protein and in baculovirus/insect cell system, site directed mutagenesis to avoid recognition problems of selenocysteine encoded by TGA stop codon in the expression systems, mutant has reduced specific acitvity
U46C
-
selenocysteine 46 to cysteine mutant, crystallization data
C82S
-
in the presence of thioredixin system, wild-type Hyr1 can consume H2O2 rapidly, while the C82S mutant totally abolish the activity, indicating the important role of Cys82 in Trx2 specifity
Sec43Cys
-
mutation of the selenium cysteine in the active site
Sec43Ser
-
mutation of the selenium cysteine in the active site
additional information