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Literature summary for 1.11.1.12 extracted from
- Purification and physicochemical characterization of a recombinant phospholipid hydroperoxide glutathione peroxidase from Oryza sativa (2007), J. Biochem. Mol. Biol., 40, 412-418.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the entire encoding region for Oryza sativa PHGPx is expressed in Escherichia coli M15 | Oryza sativa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00014 | - |
1-palmitoyl-2-(13-hydroperoxy-cis-9,trans-11-octadecadienoyl)-L-3-phosphatidylcholine | cosubstrate: glutathione | Oryza sativa |  |
| 0.0362 | - |
1-palmitoyl-2-(13-hydroperoxy-cis-9,trans-11-octadecadienoyl)-L-3-phosphatidylcholine | cosubstrate: thioredoxin | Oryza sativa | |
| 0.799 | - |
H2O2 | cosubstrate: glutathione | Oryza sativa | |
| 4.344 | - |
tert-butyl hydroperoxide | cosubstrate: glutathione | Oryza sativa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21000 | - |
x * 21000, recombinant enzyme containing a six-histidine tag at its N-terminus, SDS-PAGE | Oryza sativa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oryza sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutathione + 1-palmitoyl-2-(13-hydroperoxy-cis-9,trans-11-octadecadienoyl)-L-3-phosphatidylcholine | - |
Oryza sativa | ? | - |
? | |
| glutathione + H2O2 | - |
Oryza sativa | ? | - |
? | |
| glutathione + tert-butyl hydroperoxide | - |
Oryza sativa | ? | - |
? | |
| thioredoxin + 1-palmitoyl-2-(13-hydroperoxy-cis-9,trans-11-octadecadienoyl)-L-3-phosphatidylcholine | - |
Oryza sativa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 21000, recombinant enzyme containing a six-histidine tag at its N-terminus, SDS-PAGE | Oryza sativa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| OsPHGPx | - |
Oryza sativa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 27 | - |
- |
Oryza sativa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 40 | 10°C: 80% of maximal activity, 40°C: 60% of maximal activity | Oryza sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.3 | - |
- |
Oryza sativa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 11 | pH 8.0: about 40% of maximal activity, pH 11: about 80% of maximal activity | Oryza sativa |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Oryza sativa | isoelectric focusing electrophoresis | - |
8.5 |
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