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2'-deoxyadenosine + H2O
D-ribose + ?
-
-
-
-
?
2'-O-methyladenosine + H2O
2-O-methylribose + adenine
-
-
-
-
?
2'-O-methylcytidine + H2O
2-O-methylribose + cytosine
-
-
-
-
?
2'-O-methylguanosine + H2O
2-O-methylribose + guanidine
-
-
-
-
?
2'-O-methylinosine + H2O
2-O-methylribose + hypoxanthine
-
-
-
-
?
2'-O-methyluridine + H2O
2-O-methylribose + uracil
-
-
-
-
?
2,6-diamino-9-beta-D-ribofuranosylpurine + H2O
2,6-diaminopurine + D-ribose
-
76% of the activity with inosine
-
-
ir
2,8-diamino-9-beta-D-ribofuranosyladenine + H2O
2,8-diaminoadenine + D-ribose
-
38% of the activity with inosine
-
-
ir
2-aminopurine riboside + H2O
2-aminopurine + D-ribose
2-hydroxy-9-beta-D-ribofuranosyladenine + H2O
2-hydroxyadenine + D-ribose
-
10% of the activity with inosine
-
-
ir
3'-AMP + H2O
adenine + D-ribose 3-phosphate
E15229
0.67% of the activity with adenosine
-
-
?
3'-deoxyadenosine + H2O
D-ribose + ?
-
-
-
-
?
3-deazaadenosine + H2O
D-ribose + 3-deazaadenine
-
-
-
-
?
4-nitrophenyl beta-D-ribofuranoside + H2O
4-nitrophenol + beta-D-ribofuranose
-
-
-
-
?
4-nitrophenyl beta-D-ribofuranoside + H2O
4-nitrophenol + D-ribose
4-nitrophenylriboside + H2O
4-nitrophenol + D-ribose
-
-
-
-
?
4-nitrophenylriboside + H2O
D-ribose + 4-nitrophenol
-
-
-
?
5'-AMP + H2O
adenine + D-ribose 5-phosphate
-
-
-
-
?
5'-deoxyadenosine + H2O
5-deoxy-D-ribose + adenine
-
-
-
-
?
5'-deoxyadenosine + H2O
D-ribose + ?
-
-
-
-
?
5'-GMP + H2O
guanine + D-ribose 5-phosphate
5'-IMP + H2O
hypoxanthine + D-ribose 5-phosphate
-
-
-
-
?
5,6-dimethyl-1-beta-D-ribofuranosylbenzimidazole + H2O
5,6-dimethylbenzimidazole + D-ribose
-
200% of the activity with inosine
-
-
ir
5-methyluridine + H2O
5-methyluracil + D-ribose
6-dimethylamino-9-beta-D-ribofuranosylbenzimidazole + H2O
6-dimethylaminobenzimidazole + D-ribose
-
30% of the activity with inosine
-
-
ir
6-mercaptopurine ribonucleoside + H2O
6-mercaptopurine + ribose
-
-
-
-
?
7-methyl guanosine + H2O
D-ribose + 7-methyl guanine
7-methylguanosine + H2O
?
-
-
-
-
?
7-methylguanosine + H2O
D-ribose + 7-methylguanine
-
-
-
?
9-beta-D-ribofuranosylpurine + H2O
purine + D-ribose
-
47% of the activity with inosine
-
-
ir
adenosine + H2O
adenine + D-ribose
adenosine + H2O
D-ribose + adenine
adenosine 5'-phosphate + H2O
?
-
31% of the activity with inosine
-
-
?
cytidine + H2O
cytosine + D-ribose
cytidine + H2O
D-ribose + cytosine
cytosine + D-ribose
cytidine + H2O
-
-
-
-
r
deamino-NAD+ + H2O
?
-
-
-
-
?
ethenoadenosine + H2O
D-ribose + ethenoadenine
-
-
-
-
?
guanosine + H2O
D-ribose + guanine
guanosine + H2O
guanine + D-ribose
imidazoleacetic acid riboside + H2O
imidazoleacetate + D-ribose
-
5% of the activity with inosine
-
-
ir
inosine + H2O
D-ribose + hypoxanthine
inosine + H2O
hypoxanthine + D-ribose
NAD+ + H2O
adenine + ?
-
-
-
?
nicotinamide riboside + H2O
nicotinamide + D-ribose
-
150% of the activity with inosine
-
-
ir
p-nitrophenylriboside + H2O
4-nitrophenol + D-ribose
-
-
-
-
?
p-nitrophenylriboside + H2O
p-nitrophenol + D-ribose
-
-
-
?
purine ribonucleoside + H2O
purine + D-ribose
purine riboside + H2O
purine + D-ribose
thymidine + H2O
D-ribose + thymine
-
-
-
-
?
uridine + H2O
D-ribose + uracil
uridine + H2O
uracil + D-ribose
xanthosine + H2O
D-ribose + xanthine
xanthosine + H2O
xanthine + D-ribose
additional information
?
-
2-aminopurine riboside + H2O
2-aminopurine + D-ribose
-
-
-
-
?
2-aminopurine riboside + H2O
2-aminopurine + D-ribose
-
-
-
?
4-nitrophenyl beta-D-ribofuranoside + H2O
4-nitrophenol + D-ribose
-
-
-
-
?
4-nitrophenyl beta-D-ribofuranoside + H2O
4-nitrophenol + D-ribose
-
-
-
?
5'-GMP + H2O
guanine + D-ribose 5-phosphate
-
-
-
-
?
5'-GMP + H2O
guanine + D-ribose 5-phosphate
-
-
-
-
?
5-methyluridine + H2O
5-methyluracil + D-ribose
-
-
-
-
?
5-methyluridine + H2O
5-methyluracil + D-ribose
-
-
-
-
?
7-methyl guanosine + H2O
D-ribose + 7-methyl guanine
-
-
-
-
?
7-methyl guanosine + H2O
D-ribose + 7-methyl guanine
-
-
-
?
adenosine + H2O
adenine + D-ribose
E15229
-
-
-
?
adenosine + H2O
adenine + D-ribose
-
-
-
-
?
adenosine + H2O
adenine + D-ribose
-
-
-
-
?
adenosine + H2O
adenine + D-ribose
-
-
-
-
?
adenosine + H2O
adenine + D-ribose
-
-
-
-
?
adenosine + H2O
adenine + D-ribose
-
-
-
-
?
adenosine + H2O
adenine + D-ribose
-
-
-
-
?
adenosine + H2O
adenine + D-ribose
-
-
-
-
?
adenosine + H2O
adenine + D-ribose
-
-
-
?
adenosine + H2O
adenine + D-ribose
preferred substrate
-
-
?
adenosine + H2O
D-ribose + adenine
-
-
-
-
?
adenosine + H2O
D-ribose + adenine
-
15% of the activity with inosine
-
-
?
adenosine + H2O
D-ribose + adenine
-
-
-
-
?
adenosine + H2O
D-ribose + adenine
-
-
-
-
?
adenosine + H2O
D-ribose + adenine
-
-
-
?
adenosine + H2O
D-ribose + adenine
-
-
-
?
adenosine + H2O
D-ribose + adenine
-
-
-
-
?
adenosine + H2O
D-ribose + adenine
-
best substrate
-
-
?
adenosine + H2O
D-ribose + adenine
-
103% of the activity with inosine
-
-
ir
adenosine + H2O
D-ribose + adenine
-
no activity
-
-
?
adenosine + H2O
D-ribose + adenine
-
IAG-NH shows higher affinity for adenosine with respect to inosine and guanosine, but the catalytic efficiency of these substrates is comparable
-
-
?
adenosine + H2O
D-ribose + adenine
-
-
-
-
?
adenosine + H2O
D-ribose + adenine
-
-
-
-
?
adenosine + H2O
D-ribose + adenine
-
-
-
-
?
adenosine + H2O
D-ribose + adenine
-
substrate for inosine-adenosine-guanosine nucleoside hydrolase
-
-
?
adenosine + H2O
D-ribose + adenine
-
-
-
-
?
adenosine + H2O
D-ribose + adenine
-
-
-
?
adenosine + H2O
D-ribose + adenine
-
3.6% of the activity with xanthosine
-
-
?
cytidine + H2O
cytosine + D-ribose
-
-
-
-
?
cytidine + H2O
cytosine + D-ribose
-
-
-
-
?
cytidine + H2O
cytosine + D-ribose
-
about 20% of the rate with adenosine
-
-
?
cytidine + H2O
cytosine + D-ribose
-
-
-
-
r
cytidine + H2O
cytosine + D-ribose
-
-
-
-
?
cytidine + H2O
cytosine + D-ribose
-
-
-
?
cytidine + H2O
D-ribose + cytosine
-
isoform rih1
-
-
?
cytidine + H2O
D-ribose + cytosine
-
54% of the activity with inosine
-
-
ir
cytidine + H2O
D-ribose + cytosine
-
-
-
-
?
cytidine + H2O
D-ribose + cytosine
-
-
-
-
?
cytidine + H2O
D-ribose + cytosine
-
-
-
-
?
guanosine + H2O
D-ribose + guanine
-
-
-
-
?
guanosine + H2O
D-ribose + guanine
-
preferred substrate
-
-
?
guanosine + H2O
D-ribose + guanine
-
33% of the activity with inosine
-
-
?
guanosine + H2O
D-ribose + guanine
-
-
-
-
?
guanosine + H2O
D-ribose + guanine
-
-
-
-
?
guanosine + H2O
D-ribose + guanine
-
-
-
?
guanosine + H2O
D-ribose + guanine
-
-
-
?
guanosine + H2O
D-ribose + guanine
-
-
-
-
?
guanosine + H2O
D-ribose + guanine
-
-
-
-
?
guanosine + H2O
D-ribose + guanine
-
about 80% of the rate with adenosine
-
-
?
guanosine + H2O
D-ribose + guanine
-
90% of the activity with inosine
-
-
ir
guanosine + H2O
D-ribose + guanine
-
-
-
-
?
guanosine + H2O
D-ribose + guanine
-
-
-
-
?
guanosine + H2O
D-ribose + guanine
-
highest activity
-
-
?
guanosine + H2O
D-ribose + guanine
-
highest activity
-
-
?
guanosine + H2O
D-ribose + guanine
-
-
-
-
?
guanosine + H2O
D-ribose + guanine
-
-
-
-
?
guanosine + H2O
D-ribose + guanine
-
-
-
-
?
guanosine + H2O
D-ribose + guanine
-
-
-
-
?
guanosine + H2O
D-ribose + guanine
-
preferred substrate
-
-
?
guanosine + H2O
D-ribose + guanine
-
-
-
-
?
guanosine + H2O
D-ribose + guanine
-
-
-
-
?
guanosine + H2O
D-ribose + guanine
-
-
-
?
guanosine + H2O
D-ribose + guanine
-
1.4% of the activity with xanthosine
-
-
?
guanosine + H2O
guanine + D-ribose
E15229
29.5% of the activity with adenosine
-
-
?
guanosine + H2O
guanine + D-ribose
-
-
-
-
?
guanosine + H2O
guanine + D-ribose
-
-
-
-
?
guanosine + H2O
guanine + D-ribose
-
-
-
-
?
guanosine + H2O
guanine + D-ribose
-
-
-
-
?
guanosine + H2O
guanine + D-ribose
-
-
-
?
guanosine + H2O
guanine + D-ribose
preferred substrate
-
-
?
inosine + H2O
D-ribose + hypoxanthine
-
-
-
-
?
inosine + H2O
D-ribose + hypoxanthine
-
preferred substrate
-
-
?
inosine + H2O
D-ribose + hypoxanthine
-
-
-
?
inosine + H2O
D-ribose + hypoxanthine
-
-
-
?
inosine + H2O
D-ribose + hypoxanthine
87% of the activity with xanthosine
-
-
?
inosine + H2O
D-ribose + hypoxanthine
Physcomitrium patens Gransden 2004
87% of the activity with xanthosine
-
-
?
inosine + H2O
D-ribose + hypoxanthine
-
-
-
-
?
inosine + H2O
D-ribose + hypoxanthine
-
-
-
-
?
inosine + H2O
D-ribose + hypoxanthine
-
preferred substrate
-
-
?
inosine + H2O
D-ribose + hypoxanthine
-
-
-
-
?
inosine + H2O
D-ribose + hypoxanthine
-
-
-
?
inosine + H2O
D-ribose + hypoxanthine
best substrate
-
-
?
inosine + H2O
hypoxanthine + D-ribose
-
-
-
-
?
inosine + H2O
hypoxanthine + D-ribose
-
-
-
-
?
inosine + H2O
hypoxanthine + D-ribose
-
-
-
-
?
inosine + H2O
hypoxanthine + D-ribose
-
-
-
-
?
inosine + H2O
hypoxanthine + D-ribose
E15229
78.9% of the activity with adenosine
-
-
?
inosine + H2O
hypoxanthine + D-ribose
-
-
-
-
?
inosine + H2O
hypoxanthine + D-ribose
-
-
-
-
?
inosine + H2O
hypoxanthine + D-ribose
-
-
-
-
?
inosine + H2O
hypoxanthine + D-ribose
-
kinetic mechanism is rapid equilibrium with random product release
-
-
?
inosine + H2O
hypoxanthine + D-ribose
-
rate of hydrolysis similar to adenosine
-
-
?
inosine + H2O
hypoxanthine + D-ribose
-
-
-
?
inosine + H2O
hypoxanthine + D-ribose
-
-
-
?
inosine + H2O
hypoxanthine + D-ribose
-
-
-
ir
inosine + H2O
hypoxanthine + D-ribose
-
-
-
-
?
inosine + H2O
hypoxanthine + D-ribose
-
-
-
-
?
inosine + H2O
hypoxanthine + D-ribose
-
-
-
?
inosine + H2O
hypoxanthine + D-ribose
-
-
-
?
inosine + H2O
hypoxanthine + D-ribose
-
-
-
-
?
inosine + H2O
hypoxanthine + D-ribose
-
-
-
-
?
inosine + H2O
hypoxanthine + D-ribose
-
-
-
-
?
inosine + H2O
hypoxanthine + D-ribose
-
-
-
-
?
inosine + H2O
hypoxanthine + D-ribose
-
-
-
-
?
inosine + H2O
hypoxanthine + D-ribose
-
-
-
-
?
inosine + H2O
hypoxanthine + D-ribose
-
kinetic mechanism is rapid equilibrium with random product release
-
-
?
inosine + H2O
hypoxanthine + D-ribose
-
-
-
-
?
inosine + H2O
hypoxanthine + D-ribose
-
-
-
-
?
inosine + H2O
hypoxanthine + D-ribose
-
-
-
?
inosine + H2O
hypoxanthine + D-ribose
preferred substrate
-
-
?
inosine + H2O
hypoxanthine + D-ribose
-
25% of the activity with xanthosine
-
-
?
purine ribonucleoside + H2O
purine + D-ribose
-
-
-
-
?
purine ribonucleoside + H2O
purine + D-ribose
-
-
-
-
?
purine ribonucleoside + H2O
purine + D-ribose
-
-
-
-
?
purine riboside + H2O
purine + D-ribose
-
-
-
-
?
purine riboside + H2O
purine + D-ribose
-
-
-
-
?
purine riboside + H2O
purine + D-ribose
-
-
-
-
?
uridine + H2O
D-ribose + uracil
-
substrate for isoform URH1, but nor URH2
-
-
?
uridine + H2O
D-ribose + uracil
-
isoform rih1
-
-
?
uridine + H2O
D-ribose + uracil
-
-
-
-
?
uridine + H2O
D-ribose + uracil
-
about 15% of the rate with adenosine
-
-
?
uridine + H2O
D-ribose + uracil
-
-
-
ir
uridine + H2O
D-ribose + uracil
-
-
-
-
?
uridine + H2O
D-ribose + uracil
-
-
-
-
?
uridine + H2O
D-ribose + uracil
-
-
-
-
?
uridine + H2O
D-ribose + uracil
-
-
-
-
?
uridine + H2O
uracil + D-ribose
-
-
-
-
?
uridine + H2O
uracil + D-ribose
-
-
-
-
?
uridine + H2O
uracil + D-ribose
-
-
-
-
?
uridine + H2O
uracil + D-ribose
-
-
-
?
xanthosine + H2O
D-ribose + xanthine
-
-
-
-
?
xanthosine + H2O
D-ribose + xanthine
-
no activity
-
-
?
xanthosine + H2O
D-ribose + xanthine
-
no activity
-
-
?
xanthosine + H2O
D-ribose + xanthine
best substrate
-
-
?
xanthosine + H2O
D-ribose + xanthine
Physcomitrium patens Gransden 2004
best substrate
-
-
?
xanthosine + H2O
D-ribose + xanthine
-
-
-
-
?
xanthosine + H2O
D-ribose + xanthine
-
-
-
-
?
xanthosine + H2O
D-ribose + xanthine
70% of the activity with inosine
-
-
?
xanthosine + H2O
xanthine + D-ribose
E15229
63.5% of the activity with adenosine
-
-
?
xanthosine + H2O
xanthine + D-ribose
-
-
-
-
?
xanthosine + H2O
xanthine + D-ribose
-
-
-
-
?
xanthosine + H2O
xanthine + D-ribose
-
-
-
-
?
additional information
?
-
-
no hydrolysis of the pyrimidines uridine and cytidine
-
-
?
additional information
?
-
hydrolase activity could not be detected when uridine or cytidine is used as a substrate
-
-
?
additional information
?
-
-
hydrolase activity could not be detected when uridine or cytidine is used as a substrate
-
-
?
additional information
?
-
IunH can hydrolyse inosine, adenosine and guanosine with the activity order: inosine -
-
?
additional information
?
-
-
IunH can hydrolyse inosine, adenosine and guanosine with the activity order: inosine -
-
?
additional information
?
-
-
isoform rih1 shows pyrimidine-specific ribonuclease activity. Activity of isoform rih2 in decreasing order: inosine, adenine, uridine, guanosine, xanthosine, cytidine
-
-
?
additional information
?
-
-
purine metabolizing enzyme
-
-
?
additional information
?
-
-
enzyme of purine-metabolism
-
-
?
additional information
?
-
-
enzyme of purine catabolism
-
-
?
additional information
?
-
-
enzyme of purine-metabolism
-
-
?
additional information
?
-
-
enzyme of purine-metabolism
-
-
?
additional information
?
-
-
enzyme of purine catabolism
-
-
?
additional information
?
-
-
enzyme of purine-metabolism
-
-
?
additional information
?
-
-
enzyme of purine-metabolism
-
-
?
additional information
?
-
-
the enzyme prefers ribonucleosides and 2'-O-methylribonucleosides as its hydrolytic substrates, but reacts weakly with 2'-deoxyribonucleosides
-
-
?
additional information
?
-
poor activity with adenosine, uridine, and guanosine, no activity with cytokinin riboside, cytidine, and trans-zeatin riboside. Substrate docking into the active site, overview
-
-
?
additional information
?
-
-
poor activity with adenosine, uridine, and guanosine, no activity with cytokinin riboside, cytidine, and trans-zeatin riboside. Substrate docking into the active site, overview
-
-
?
additional information
?
-
Physcomitrium patens Gransden 2004
poor activity with adenosine, uridine, and guanosine, no activity with cytokinin riboside, cytidine, and trans-zeatin riboside. Substrate docking into the active site, overview
-
-
?
additional information
?
-
-
enzyme of purine catabolism
-
-
?
additional information
?
-
-
IAG-NH does not recognize as substrate nucleosides 5'-phosphates, NAD+ and NADP+
-
-
?
additional information
?
-
the enzyme catalyzes the irreversible hydrolysis of the N-glycosidic bond of beta-ribonucleosides forming ribose and purine base, substrate specificity and involved residues, overview
-
-
?
additional information
?
-
-
IAG-NH does not recognize as substrate nucleosides 5'-phosphates, NAD+ and NADP+
-
-
?
additional information
?
-
-
cytidine, uridine, and 4-nitrophenyl-riboside are poor substrates
-
-
?
additional information
?
-
-
inosine-guanosine nucleoside hydrolase does not use adenosine as substrate
-
-
?
additional information
?
-
-
cytidine, uridine, and 4-nitrophenyl-riboside are poor substrates
-
-
?
additional information
?
-
-
enzyme of purine-metabolism
-
-
?
additional information
?
-
poor activity with adenosine, uridine, guanosine, trans-zeatin riboside, and cytokinin riboside, almost no activity with cytidine. Substrate docking into the active site, overview
-
-
?
additional information
?
-
-
poor activity with adenosine, uridine, guanosine, trans-zeatin riboside, and cytokinin riboside, almost no activity with cytidine. Substrate docking into the active site, overview
-
-
?
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(+-)-1-[(9-deazahypoxanthin-9-yl)methyl]azetidine-2-methanol
(1S)-(1-aminonaphthalen-4-yl)-1,4-dideoxy-1,4-imino-D-ribitol
-
-
(1S)-1,4-dideoxy-1-(2-guanidinoethyl)-1,4-imino-D-ribitol
-
-
(1S)-1-(2,3-diamino-5-pyridyl)-1,4-dideoxy-1,4-imino-D-ribitol
-
-
(1S)-1-(3,4-diaminophenyl)-1,4-dideoxy-1,4-imino-D-ribitol
-
-
(1S)-1-(4-aminomethyl)phenyl-1,4-dideoxy-1,4-imino-D-ribitol
-
-
(1S)-1-(4-cyanophenyl)1,4-dideoxy-1,4-imino-D-ribitol
-
-
(2R,3R,4S)-1-benzyl-2-(hydroxymethyl)pyrrolidine-3,4-diol
-
(2R,3R,4S)-1-[(4-amino-4,5-dihydro-3H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-2-(hydroxymethyl)pyrrolidine-3,4-diol
-
(2R,3R,4S)-1-[(4-hydroxy-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-2-(hydroxymethyl)pyrrolidin-3,4-diol
nanomolar inhibitor
(2R,3R,4S)-2-(hydroxymethyl)-1-(4-iodobenzyl)pyrrolidine-3,4-diol
-
(2R,3R,4S)-2-(hydroxymethyl)-1-(4-nitrobenzyl)pyrrolidine-3,4-diol
-
(2R,3R,4S)-2-(hydroxymethyl)-1-(quinolin-8-ylmethyl)pyrrolidin-3,4-diol
nanomolar inhibitor
(2R,3R,4S)-2-(hydroxymethyl)-1-(quinolin-8-ylmethyl)pyrrolidine-3,4-diol
-
(2R,3R,4S,5S)-2-(hydroxymethyl)-5-phenylpyrrolidine-3,4-diol
-
(2S,3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-2-(hydroxymethyl)pyrrolidine-3,4-diol
nanomolar inhibitor
1,10-phenanthroline
E15229
1 mM, 23% inhibition
1,2,5-trideoxy-1-(guanin-9-yl)-2,5-imino-D-allitol
-
-
1,2,5-trideoxy-1-(hypoxanthin-9-yl)-2,5-imino-D-alitol
-
-
1,4-dideoxy-(3-guanidinopropyl)-1,4-imino-D-ribitol
-
-
1,4-dideoxy-1,4-imino-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(1-isoquinolinyl)methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(1-methyl-1H-1,2,3-benzotriazol-5-yl)methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(1-methyl-1H-benzimidazol-2-yl)methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(2(1H)-quinolon-8-yl)methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(2-amino-4-benzothiazoyl)-methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(2-aminopyridin-3-yl)methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(2-aminoquinolin-8-yl)methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(2-chloroquinolin-8-yl)methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(2-methoxyquinolin-8-yl)methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(2-naphthyl)methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(2-quinolinyl)methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(3-cyanobenzyl)-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(3-indolyl)methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(3-nitrobenzyl)-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(3-quinolinyl)methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(4(1H)-quinolon-8-yl)methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(4-amidinobenzyl)-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(4-amino-2-methylquinolin-8-yl)methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(4-aminoquinazolin-8-yl)methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(4-aminoquinolin-8-yl)methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(4-aminothieno[3,2-d]pyrimidin-7-yl)-methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(4-chloro-2-methylquinolin-8-yl)methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(4-chloroquinolin-8-yl)methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(4-cyanobenzyl)-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(4-guanidinobenzyl)-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(4-methoxyquinolin-8-yl)methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(4-methyl-2-phenylpyrimidin-5-yl)methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(4-pyridinyl)methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(4-quinolinyl)methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(6-methoxypyridin-3-yl)methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(7-methoxy-indol-3-yl)methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(7-quinolinyl)methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(7-thieno[3,2-d]pyrimidonyl)-methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(8-hydroxyquinolin-2-yl)methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(8-quinolinyl)methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(quinazolin-8-yl)methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(quinazolon-8-yl)methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(quinoxalin-5-yl)-methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-(thieno[3,2-d]pyrimidin-7-yl)methyl-D-ribitol
-
1,4-dideoxy-1,4-imino-N-[3-(1H-pyrrol-1-yl)benzyl]-D-ribitol
-
1,4-dideoxy-1,4-imino-N-[4-(1H-1,2,4-triazol-1-yl)benzyl]-D-ribitol
-
1,4-dideoxy-1,4-imino-N-[4-(1H-pyrazol-1-yl)benzyl]-D-ribitol
-
1,4-dideoxy-1,4-imino-N-[4-(1H-pyrrol-1-yl)benzyl]-D-ribitol
-
1-(5-carboxamido-2-pyridyl)-1,2,5-trideoxy-2,5-imino-D-allitol
-
-
1-(adenin-9-yl)-1,2,5-trideoxy-2,5-imino-D-allitol
-
-
1-guanidino-2,5-anhydro-1-deoxy-D-allitol
-
-
1-guanidino-3,6-anhydro-1,2-dideoxy-D-allo-heptitol
-
-
1-[(7-benzyloxymethyl-6-tert-butoxy-9-deazapurin-9-yl)methyl] azetidine-3-methanol
1-[(9-deazahypoxanthin-9-yl)methyl]azetidine-3,3dimethanol
1-[2-[(2S,3S,4R,5R)-3,4-dihydroxy-5-(hydroxymethyl)pyrrolidin-2-yl]ethyl]guanidine
-
1-[3-[(2R,3R,4S)-3,4-dihydroxy-2-(hydroxymethyl)pyrrolidin-1-yl]propyl]guanidine
-
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin H
7-[[(2R,3R,4S)-3,4-dihydroxy-2-(hydroxymethyl)pyrrolidin-1-yl]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
8-aza-immucillin-H
-
nanomolar inhibitor
8-hydroxyquinoline
E15229
1 mM, 93% inhibition
Ag+
E15229
1 mM, 68% inhibition
Caffeine
-
weak competitive inhibitor
Co2+
-
mixed-type inhibition
cytidine
E15229
0.5 mM, 81% inhibition of activity with adenosine, 92% inhibition of activity with inosine, 91% inhibition of the activity with xanthosine, 94% inhibition of activity with guanosine
daidzein
-
enzyme is a major daidzein-binding protein
GMP
-
substrate inhibition above 1 mM
guanosine
-
competitive inhibitor of xanthosine hydrolysis
Hg2+
E15229
1 mM,61% inhibition
hypoxanthine arabinoside
-
-
iminoribitols
-
poorly inhibited by free or substituted iminoribitols
-
Inosine
-
competitive inhibitor of xanthosine hydrolysis
iodoacetate
-
0.01 M, 25% inhibition. 0.02 M 36.1% inhibition
Li2SO4
-
inhibits activity with adenosine and guanosine, slight inhibition of activity with inosine
meso-[(9-deazahypoxanthin-9-yl)methyl]azetidine-2,4-dimethanol hydrochloride
Methylxanthine
-
weak competitive inhibitor
MnSO4
-
inhibits activity with adenosine and guanosine, very slight inhibition of activity with inosine
N-(9-deaza-adenin-9-yl)methyl-1,4-dideoxy-1,4-imino-D-ribitol
N-(9-deazaadenin-9-yl)methyl-1,4-dideoxy-1,4-imino-D-ribitol
-
N-(9-deazahypoxanthin-9-yl)methyl-1,4-dideoxy-1,4-imino-D-ribitol
-
N-benzyl-1,4-dideoxy-1,4-imino-D-ribitol
-
-
Ni2+
-
mixed-type inhibition
purine 2'-deoxyribonucleoside
-
-
pyrazolo(3,4-d)pyrimidine
-
-
Zn2+
-
mixed-type inhibition
(+-)-1-[(9-deazahypoxanthin-9-yl)methyl]azetidine-2-methanol
-
transition state analogue inhibitor, comparison with inhibition of human and Plasmodium falciparum enzyme
(+-)-1-[(9-deazahypoxanthin-9-yl)methyl]azetidine-2-methanol
-
transition state analogue inhibitor, comparison with inhibition of bovine and Plasmodium falciparum enzyme
(+-)-1-[(9-deazahypoxanthin-9-yl)methyl]azetidine-2-methanol
-
transition state analogue inhibitor, comparison with inhibition of human and bovine enzyme
1-[(7-benzyloxymethyl-6-tert-butoxy-9-deazapurin-9-yl)methyl] azetidine-3-methanol
-
transition state analogue inhibitor, comparison with inhibition of human and Plasmodium falciparum enzyme
1-[(7-benzyloxymethyl-6-tert-butoxy-9-deazapurin-9-yl)methyl] azetidine-3-methanol
-
transition state analogue inhibitor, comparison with inhibition of bovine and Plasmodium falciparum enzyme
1-[(7-benzyloxymethyl-6-tert-butoxy-9-deazapurin-9-yl)methyl] azetidine-3-methanol
-
transition state analogue inhibitor, comparison with inhibition of human and bovine enzyme. Ki value above 10 microM
1-[(9-deazahypoxanthin-9-yl)methyl]azetidine-3,3dimethanol
-
transition state analogue inhibitor, comparison with inhibition of human and Plasmodium falciparum enzyme
1-[(9-deazahypoxanthin-9-yl)methyl]azetidine-3,3dimethanol
-
transition state analogue inhibitor, comparison with inhibition of bovine and Plasmodium falciparum enzyme
1-[(9-deazahypoxanthin-9-yl)methyl]azetidine-3,3dimethanol
-
transition state analogue inhibitor, comparison with inhibition of human and bovine enzyme, Ki value above 10 microM
2'-deoxyadenosine
-
-
3-deazaadenosine
-
-
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin H
-
i.e. DADMe-ImmH, transition state analogue inhibitor, comparison with inhibition of human and Plasmodium falciparum enzyme
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin H
-
i.e. DADMe-ImmH, transition state analogue inhibitor, comparison with inhibition of bovine and Plasmodium falciparum enzyme
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin H
-
7-deazaadenosine
-
i.e. tubercidin
7-deazaadenosine
-
i.e. tubercidin
adenosine
-
potent
adenosine
-
competitive inhibitor of xanthosine hydrolysis and inosine hydrolysis
Cu2+
E15229
1 mM, 57% inhibition
Cu2+
-
non-competitive inhibition
D-ribose
-
product inhibition
D-ribose
-
product inhibition
EDTA
-
0.01 M, 28% inhibition. 0.02 M 42% inhibition
EDTA
E15229
1 mM, 46% inhibition
EDTA
-
after 1 h incubation with EDTA (5 mM) the enzyme activity is reduced to less than 0.05% and is restored by addition of 20 mM CaCl2
HgCl2
-
10 mM, 17% inhibition
hypoxanthine
-
product inhibition
immucillin A
-
-
immucillin H
-
-
immucillin H
-
transition-state inhibitor, i.e. (1S)-1-(9-deazahypoxanthin-9-yl)-1,4-dideoxy-1,4-imino-D-ribitol
immucillin H
immucilin H is (1S)-1-(9-deazahypoxanthin-9-yl)-1,4-dideoxy-1,4-imino-D-ribitol
immucillin H
immucillin H is (1S)-1-(9-deazaadenin-9-yl)-1,4-dideoxy-1,4-imino-D-ribitol
immucillin-A
-
competitive inhibition
immucillin-A
-
nanomolar inhibitor
immucillin-H
-
nanomolar inhibitor
meso-[(9-deazahypoxanthin-9-yl)methyl]azetidine-2,4-dimethanol hydrochloride
-
transition state analogue inhibitor, comparison with inhibition of human and Plasmodium falciparum enzyme
meso-[(9-deazahypoxanthin-9-yl)methyl]azetidine-2,4-dimethanol hydrochloride
-
transition state analogue inhibitor, comparison with inhibition of bovine and Plasmodium falciparum enzyme
meso-[(9-deazahypoxanthin-9-yl)methyl]azetidine-2,4-dimethanol hydrochloride
-
transition state analogue inhibitor, comparison with inhibition of human and bovine enzyme
Mn2+
E15229
1 mM, 34% inhibition
Mn2+
-
mixed-type inhibition
N-(9-deaza-adenin-9-yl)methyl-1,4-dideoxy-1,4-imino-D-ribitol
-
UAMC-00363, is the only N-arylmethyl-substituted iminoribitol derivative active as chemotherapeutic agent against trypanosomiasis, validation in an in vivo model of African trypanosomiasis. No cross-resistance to the diamidine or the melaminophenyl arsenical classes of trypanocides
N-(9-deaza-adenin-9-yl)methyl-1,4-dideoxy-1,4-imino-D-ribitol
-
UAMC-00363
UAMC-00109
-
-
UAMC-00115
-
-
UAMC-00218
-
-
UAMC-00311
-
-
UAMC-00312
-
competitive inhibition
UAMC-00346
-
-
UAMC-00363
-
a trypanocidal compound, competitive inhibition
UAMC-00363
-
i.e. N-(9-deaza-adenin-9-yl)methyl-1,4-dideoxy-1,4-imino-D-ribitol, significant inhibition of inosine-adenosine-guanosine nucleoside hydrolase and inosine-guanosine nucleoside hydrolase
UAMC-00363
-
i.e. N-(9-deaza-adenin-9-yl)methyl-1,4-dideoxy-1,4-imino-D-ribitol
UAMC-00375
-
-
uridine
E15229
0.5 mM, 38% inhibition of activity with adenosine, 45% inhibition of activity with inosine, 35% inhibition of the activity with xanthosine, 58% inhibition of activity with guanosine
uridine
-
competitive inhibitor of xanthosine hydrolysis
Xanthosine
-
moderate
Xanthosine
-
competitive inhibitor of inosine hydrolysis
additional information
-
transition state analogue inhibitors of protozoan nucleoside hydrolases
-
additional information
-
high-affinity inhibition of the enzyme by iminoribitol-based compounds. Metallorganic complexes can compete for binding at the active site of nucleoside hydrolase enzymes, mimicking the positively charged transition state of the enzymatic reaction, different modes of iminoribitol ring binding at a conserved active site, overview. Divalent metal ions can act as noncompetitive enzyme inhibitors, stabilizing a nonproductive conformation of the catalytic loop
-
additional information
in silico docking experiments using flavone, 5-hydroxyflavone, 7-hydroxyflavone, chrysin, apigenin, kaempferol, fisetin, and quercetin. All tested flavonoids show high affinities for the enzyme, the lowest free binding energy ranging from -10.23 to -7.14 kcal/mol
-
additional information
-
in silico docking experiments using flavone, 5-hydroxyflavone, 7-hydroxyflavone, chrysin, apigenin, kaempferol, fisetin, and quercetin. All tested flavonoids show high affinities for the enzyme, the lowest free binding energy ranging from -10.23 to -7.14 kcal/mol
-
additional information
-
N-arylmethyl-substituted iminoribitol derivatives as potential chemotherapeutic agents against trypanosomiasis, validation in an in vivo model of African trypanosomiasis
-
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0.042
2'-deoxyadenosine
-
-
0.04
2'-O-methyluridine
-
pH 7.0, 28°C
0.0008 - 0.009
2-aminopurine riboside
0.6
3'-deoxyadenosine
-
-
0.0022
3-deazaadenosine
-
at pH 7.0 and 35°C
0.0011
4-nitrophenyl beta-D-ribofuranoside
-
at pH 7.0 and 35°C
0.00032 - 0.009
4-nitrophenylriboside
0.24 - 9.87
5'-deoxyadenosine
0.026 - 1.3
5-methyluridine
0.0005 - 0.008
7-methyl guanosine
0.037 - 0.053
7-methylguanosine
0.0036
ethenoadenosine
-
at pH 7.0 and 35°C
0.562 - 257
p-nitrophenylriboside
0.004 - 0.011
purine ribonucleoside
0.0006 - 0.145
purine riboside
additional information
additional information
-
0.0008
2-aminopurine riboside
-
at pH 7.0 and 35°C
0.009
2-aminopurine riboside
wild type enzyme, in 50 mM potassium phosphate buffer, at 25°C and pH 7.0
0.00032
4-nitrophenylriboside
wild-type, pH 7.5, 25°C
0.00064
4-nitrophenylriboside
mutant C42A, pH 7.5, 25°C
0.009
4-nitrophenylriboside
mutant C253A, ApH 7.5, 25°C
0.24
5'-deoxyadenosine
-
at pH 7.0 and 35°C
9.87
5'-deoxyadenosine
-
-
0.026
5-methyluridine
-
-
0.0005
7-methyl guanosine
wild type enzyme, in 50 mM potassium phosphate buffer, at 25°C and pH 7.0
0.0021
7-methyl guanosine
-
wild-type, pH 7.0, 35°C
0.0024
7-methyl guanosine
-
mutant R252A, pH 7.0, 35°C
0.008
7-methyl guanosine
-
mutant D40A, pH 7.0, 35°C
0.037
7-methylguanosine
wild-type, pH 7.5, 25°C
0.051
7-methylguanosine
mutant C253A, ApH 7.5, 25°C
0.053
7-methylguanosine
mutant C42A, pH 7.5, 25°C
0.001
adenosine
-
Km less than 0.001 mM, at pH 7.0 and 35°C
0.0066
adenosine
-
mutant R252A, pH 7.0, 35°C
0.008
adenosine
-
wild-type, pH 7.0, 35°C
0.00854
adenosine
pH 7.2, 35°C, His-tagged enzyme form
0.0118
adenosine
E15229
-
0.016
adenosine
-
recombinant enzyme, pH 7.3, 37°C
0.0431
adenosine
-
at 30°C, pH not specified in the publication
0.077
adenosine
wild-type, pH 7.5, 25°C
0.094
adenosine
mutant C42A, pH 7.5, 25°C
0.096
adenosine
mutant C253A, ApH 7.5, 25°C
0.16
adenosine
-
pH 7.2, 37°C
0.106
cytidine
-
-
0.271
cytidine
-
at pH 7.0 and 35°C
0.925
cytidine
pH 7.2, 35°C, His-tagged enzyme form
0.925
cytidine
-
wild-type, pH 7.0, 35°C
1.06
cytidine
-
mutant R252A, pH 7.0, 35°C
9.2
cytidine
-
pH 7.2, 37°C
0.0018
guanosine
mutant enzyme D253P, in 50 mM potassium phosphate buffer, at 25°C and pH 7.0
0.0023
guanosine
-
wild-type, pH 7.0, 35°C
0.0023
guanosine
wild type enzyme, in 50 mM potassium phosphate buffer, at 25°C and pH 7.0
0.003
guanosine
mutant enzyme E249P, in 50 mM potassium phosphate buffer, at 25°C and pH 7.0
0.00382
guanosine
pH 7.2, 35°C, His-tagged enzyme form
0.004
guanosine
mutant enzyme C248P, in 50 mM potassium phosphate buffer, at 25°C and pH 7.0
0.0045
guanosine
-
at pH 7.0 and 35°C
0.008
guanosine
mutant enzyme H247P, in 50 mM potassium phosphate buffer, at 25°C and pH 7.0
0.016
guanosine
mutant enzyme L250P, in 50 mM potassium phosphate buffer, at 25°C and pH 7.0
0.021
guanosine
mutant enzyme L251P, in 50 mM potassium phosphate buffer, at 25°C and pH 7.0
0.024
guanosine
mutant enzyme R252P, in 50 mM potassium phosphate buffer, at 25°C and pH 7.0
0.031
guanosine
-
mutant R252A, pH 7.0, 35°C
0.054
guanosine
mutant C42A, pH 7.5, 25°C
0.0563
guanosine
E15229
-
0.066
guanosine
-
mutant D40A, pH 7.0, 35°C
0.068
guanosine
wild-type, pH 7.5, 25°C
0.075
guanosine
-
recombinant enzyme, pH 7.3, 37°C
0.3
guanosine
mutant C253A, ApH 7.5, 25°C
0.0019
Inosine
-
at pH 7.0 and 35°C
0.0052
Inosine
pH 7.2, 35°C, without His-tag
0.00537
Inosine
pH 7.2, 35°C, His-tagged enzyme form
0.051
Inosine
-
recombinant enzyme, pH 7.3, 37°C
0.07
Inosine
-
pH 7.3, 37°C
0.078
Inosine
pH 7.5, 30°C, wild-type enzyme
0.094
Inosine
pH 7.5, 30°C, mutant E247A
0.0972
Inosine
-
at 30°C, pH not specified in the publication
0.129
Inosine
wild-type, pH 7.5, 25°C
0.134
Inosine
pH 7.5, 30°C, mutant D250A
0.201
Inosine
pH 7.5, 30°C, recombinant enzyme
0.215
Inosine
mutant C42A, pH 7.5, 25°C
0.301
Inosine
pH 7.5, 30°C, mutant D252A
0.33
Inosine
mutant enzyme H79A, pH and temperature not specified in the publication
0.34
Inosine
wild-type enzyme, pH and temperature not specified in the publication
0.343
Inosine
mutant C253A, ApH 7.5, 25°C
0.399
Inosine
in 50 mM HEPES (pH 7.3), at 25°C
0.63
Inosine
pH 7.5, 30°C, mutant Y241A
0.65
Inosine
-
pH 7.2, 37°C
0.76
Inosine
pH 7.5, 30°C, mutant Y255A
1.09
Inosine
pH 7.5, 30°C, mutant H245A
1.208
Inosine
pH 7.5, 30°C, mutant Y244A
1.28
Inosine
-
pH 6.0, 37°C
1390
Inosine
pH 7.5, 30°C, mutant Y249A
0.562
p-nitrophenylriboside
-
-
257
p-nitrophenylriboside
pH 7.2, 35°C, His-tagged enzyme form
0.004
purine ribonucleoside
-
wild-type, pH 7.0, 35°C
0.011
purine ribonucleoside
-
mutant R252A, pH 7.0, 35°C
0.0006
purine riboside
-
at pH 7.0 and 35°C
0.019
purine riboside
-
-
0.145
purine riboside
-
-
0.102
uridine
-
-
0.586
uridine
pH 7.2, 35°C, His-tagged enzyme form
1.06
uridine
-
pH 7.2, 37°C
1.451
uridine
-
at pH 7.0 and 35°C
0.0158
Xanthosine
E15229
-
0.116
Xanthosine
pH 7.5, 30°C, wild-type enzyme
0.14
Xanthosine
pH 7.5, 30°C, mutant E247A
0.171
Xanthosine
pH 7.5, 30°C, mutant D250A
0.206
Xanthosine
pH 7.5, 30°C, mutant Y244A
0.232
Xanthosine
pH 7.5, 30°C, mutant D252A
0.28
Xanthosine
-
at pH 7.0 and 35°C
0.356
Xanthosine
pH 7.5, 30°C, mutant H245A
0.367
Xanthosine
pH 7.5, 30°C, mutant Y255A
0.396
Xanthosine
pH 7.5, 30°C, recombinant enzyme
0.487
Xanthosine
pH 7.5, 30°C, mutant Y241A
0.596
Xanthosine
pH 7.5, 30°C, mutant Y249A
0.79
Xanthosine
-
pH 6.0, 37°C
5.9
Xanthosine
-
pH 7.2, 37°C
additional information
additional information
substrate specificity and kinetics, overview
-
additional information
additional information
-
substrate specificity and kinetics, overview
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.49
2'-O-methyluridine
-
pH 7.0, 28°C
0.57
2-aminopurine riboside
0.009
3-deazaadenosine
-
at pH 7.0 and 35°C
0.03
4-nitrophenyl beta-D-ribofuranoside
-
at pH 7.0 and 35°C
0.017 - 0.039
4-nitrophenylriboside
0.078
5'-deoxyadenosine
-
at pH 7.0 and 35°C
1.07 - 13.6
7-methyl guanosine
1.09 - 5.2
7-methylguanosine
6.9
ethenoadenosine
-
at pH 7.0 and 35°C
0.206
p-nitrophenylriboside
pH 7.2, 35°C, His-tagged enzyme form
2 - 3.9
purine ribonucleoside
0.248
purine riboside
-
at pH 7.0 and 35°C
0.57
2-aminopurine riboside
wild type enzyme, in 50 mM potassium phosphate buffer, at 25°C and pH 7.0
0.57
2-aminopurine riboside
-
at pH 7.0 and 35°C
0.017
4-nitrophenylriboside
mutant C253A, ApH 7.5, 25°C
0.026
4-nitrophenylriboside
mutant C42A, pH 7.5, 25°C
0.039
4-nitrophenylriboside
wild-type, pH 7.5, 25°C
1.07
7-methyl guanosine
wild type enzyme, in 50 mM potassium phosphate buffer, at 25°C and pH 7.0
3.4
7-methyl guanosine
-
wild-type, pH 7.0, 35°C
13.2
7-methyl guanosine
-
mutant R252A, pH 7.0, 35°C
13.6
7-methyl guanosine
-
mutant D40A, pH 7.0, 35°C
1.09
7-methylguanosine
wild-type, pH 7.5, 25°C
1.98
7-methylguanosine
mutant C253A, ApH 7.5, 25°C
2.4
7-methylguanosine
mutant C42A, pH 7.5, 25°C
5.2
7-methylguanosine
-
at pH 7.0 and 5°C
0.0042
adenosine
mutant C253A, ApH 7.5, 25°C
0.023
adenosine
mutant C42A, pH 7.5, 25°C
0.237
adenosine
-
Km less than 0.001 mM, at pH 7.0 and 35°C
0.418
adenosine
wild-type, pH 7.5, 25°C
1.46
adenosine
pH 7.2, 35°C, His-tagged enzyme form
2.1
adenosine
-
pH 7.2, 37°C
2.6
adenosine
-
wild-type, pH 7.0, 35°C
5.7
adenosine
-
mutant R252A, pH 7.0, 35°C
17.3
adenosine
-
recombinant enzyme, pH 7.3, 37°C
0.338
cytidine
pH 7.2, 35°C, His-tagged enzyme form
0.338
cytidine
-
wild-type, pH 7.0, 35°C
0.52
cytidine
-
at pH 7.0 and 35°C
0.54
cytidine
-
mutant R252A, pH 7.0, 35°C
7.8
cytidine
-
pH 7.2, 37°C
0.0051
guanosine
mutant C253A, ApH 7.5, 25°C
0.011
guanosine
mutant C42A, pH 7.5, 25°C
0.143
guanosine
wild-type, pH 7.5, 25°C
1.09
guanosine
mutant enzyme C248P, in 50 mM potassium phosphate buffer, at 25°C and pH 7.0
1.3
guanosine
-
at pH 7.0 and 5°C
1.4
guanosine
mutant enzyme L250P, in 50 mM potassium phosphate buffer, at 25°C and pH 7.0
1.7
guanosine
mutant enzyme E249P, in 50 mM potassium phosphate buffer, at 25°C and pH 7.0
1.9
guanosine
pH 7.2, 35°C, His-tagged enzyme form
2.3
guanosine
-
wild-type, pH 7.0, 35°C
2.3
guanosine
wild type enzyme, in 50 mM potassium phosphate buffer, at 25°C and pH 7.0
2.5
guanosine
mutant enzyme L251P, in 50 mM potassium phosphate buffer, at 25°C and pH 7.0
2.7
guanosine
-
mutant D40A, pH 7.0, 35°C
3.9
guanosine
mutant enzyme D253P, in 50 mM potassium phosphate buffer, at 25°C and pH 7.0
4.1
guanosine
mutant enzyme H247P, in 50 mM potassium phosphate buffer, at 25°C and pH 7.0
9.8
guanosine
-
mutant R252A, pH 7.0, 35°C
13
guanosine
mutant enzyme R252P, in 50 mM potassium phosphate buffer, at 25°C and pH 7.0
37.3
guanosine
-
recombinant enzyme, pH 7.3, 37°C
37.6
guanosine
-
at pH 7.0 and 35°C
0.0043
Inosine
mutant C253A, ApH 7.5, 25°C
0.024
Inosine
mutant C42A, pH 7.5, 25°C
0.06
Inosine
pH 7.5, 30°C, mutant Y241A
0.203
Inosine
wild-type, pH 7.5, 25°C
0.47
Inosine
pH 7.5, 30°C, mutant Y255A
0.7
Inosine
pH 7.5, 30°C, mutant Y249A
0.85
Inosine
mutant enzyme H79A, pH and temperature not specified in the publication
1.1
Inosine
pH 7.5, 30°C, mutant H245A
1.4
Inosine
pH 7.5, 30°C, mutant Y244A
2 - 8
Inosine
-
at pH 7.0 and 35°C
3
Inosine
pH 7.5, 30°C, mutant D252A
5.19
Inosine
pH 7.2, 35°C, His-tagged enzyme form
5.49
Inosine
pH 7.2, 35°C, wiithout His-tag
5.7
Inosine
pH 7.5, 30°C, mutant E247A
6
Inosine
pH 7.5, 30°C, wild-type enzyme
7.2
Inosine
pH 7.5, 30°C, recombinant enzyme
7.2
Inosine
pH 7.5, 30°C, mutant D250A
8.1
Inosine
-
pH 6.0, 37°C
11.6
Inosine
wild-type enzyme, pH and temperature not specified in the publication
48.9
Inosine
in 50 mM HEPES (pH 7.3), at 25°C
61
Inosine
-
recombinant enzyme, pH 7.3, 37°C
2
purine ribonucleoside
-
mutant R252A, pH 7.0, 35°C
3.9
purine ribonucleoside
-
wild-type, pH 7.0, 35°C
0.0022
uridine
-
-
0.0038
uridine
-
at pH 7.0 and 35°C
0.022
uridine
pH 7.2, 35°C, His-tagged enzyme form
46
uridine
-
pH 7.2, 37°C
0.029
Xanthosine
-
-
0.19
Xanthosine
pH 7.5, 30°C, mutant Y241A
0.43
Xanthosine
pH 7.5, 30°C, mutant Y255A
0.58
Xanthosine
pH 7.5, 30°C, mutant Y244A
1.1
Xanthosine
pH 7.5, 30°C, mutant Y249A
1.3
Xanthosine
pH 7.5, 30°C, mutant H245A
2.5
Xanthosine
pH 7.5, 30°C, mutant D252A
6.2
Xanthosine
pH 7.5, 30°C, recombinant enzyme
6.2
Xanthosine
-
at pH 7.0 and 35°C
7
Xanthosine
pH 7.5, 30°C, wild-type enzyme
7.2
Xanthosine
pH 7.5, 30°C, mutant E247A
8.4
Xanthosine
pH 7.5, 30°C, mutant D250A
26.3
Xanthosine
-
pH 6.0, 37°C
62
Xanthosine
-
pH 7.2, 37°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0000018
(+-)-1-[(9-deazahypoxanthin-9-yl)methyl]azetidine-2-methanol
0.018
(1S )-1,4-dideoxy-1-(2-guanidinoethyl)-1,4-imino-D-ribitol
-
pH 7.0, 35°C
0.059
(2R,3R,4S)-1-benzyl-2-(hydroxymethyl)pyrrolidine-3,4-diol
-
0.0000041
(2R,3R,4S)-1-[(4-amino-4,5-dihydro-3H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-2-(hydroxymethyl)pyrrolidine-3,4-diol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.0000044
(2R,3R,4S)-1-[(4-hydroxy-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-2-(hydroxymethyl)pyrrolidin-3,4-diol
-
0.0043
(2R,3R,4S)-2-(hydroxymethyl)-1-(4-iodobenzyl)pyrrolidine-3,4-diol
-
0.0075
(2R,3R,4S)-2-(hydroxymethyl)-1-(4-nitrobenzyl)pyrrolidine-3,4-diol
-
0.0000108
(2R,3R,4S)-2-(hydroxymethyl)-1-(quinolin-8-ylmethyl)pyrrolidin-3,4-diol
-
0.0000108
(2R,3R,4S)-2-(hydroxymethyl)-1-(quinolin-8-ylmethyl)pyrrolidine-3,4-diol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.253
(2R,3R,4S,5S)-2-(hydroxymethyl)-5-phenylpyrrolidine-3,4-diol
-
0.0000041
(2S,3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-2-(hydroxymethyl)pyrrolidine-3,4-diol
-
0.04
1,4-dideoxy-(3-guanidinopropyl)-1,4-imino-D-ribitol
-
pH 7.0, 35°C
0.0061
1,4-dideoxy-1,4-imino-D-ribitol
-
pH 7.0, 35°C
0.00075
1,4-dideoxy-1,4-imino-N-(1-isoquinolinyl)methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.00072
1,4-dideoxy-1,4-imino-N-(1-methyl-1H-1,2,3-benzotriazol-5-yl)methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.0021
1,4-dideoxy-1,4-imino-N-(1-methyl-1H-benzimidazol-2-yl)methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.187
1,4-dideoxy-1,4-imino-N-(2(1H)-quinolon-8-yl)methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.00004
1,4-dideoxy-1,4-imino-N-(2-amino-4-benzothiazoyl)-methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.12
1,4-dideoxy-1,4-imino-N-(2-aminopyridin-3-yl)methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.00836
1,4-dideoxy-1,4-imino-N-(2-aminoquinolin-8-yl)methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.000314
1,4-dideoxy-1,4-imino-N-(2-chloroquinolin-8-yl)methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.001258
1,4-dideoxy-1,4-imino-N-(2-methoxyquinolin-8-yl)methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.002
1,4-dideoxy-1,4-imino-N-(2-naphthyl)methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.00018
1,4-dideoxy-1,4-imino-N-(2-quinolinyl)methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.0038
1,4-dideoxy-1,4-imino-N-(3-cyanobenzyl)-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.012
1,4-dideoxy-1,4-imino-N-(3-indolyl)methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.0023
1,4-dideoxy-1,4-imino-N-(3-nitrobenzyl)-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.02
1,4-dideoxy-1,4-imino-N-(3-quinolinyl)methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.0131
1,4-dideoxy-1,4-imino-N-(4(1H)-quinolon-8-yl)methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.0067
1,4-dideoxy-1,4-imino-N-(4-amidinobenzyl)-D-ribitol
-
pH 7.0, 35°C
0.0713
1,4-dideoxy-1,4-imino-N-(4-amino-2-methylquinolin-8-yl)methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.000015
1,4-dideoxy-1,4-imino-N-(4-aminoquinazolin-8-yl)methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.00005
1,4-dideoxy-1,4-imino-N-(4-aminoquinolin-8-yl)methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.000019
1,4-dideoxy-1,4-imino-N-(4-aminothieno[3,2-d]pyrimidin-7-yl)-methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.000369
1,4-dideoxy-1,4-imino-N-(4-chloro-2-methylquinolin-8-yl)methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.000014
1,4-dideoxy-1,4-imino-N-(4-chloroquinolin-8-yl)methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.026
1,4-dideoxy-1,4-imino-N-(4-cyanobenzyl)-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.007
1,4-dideoxy-1,4-imino-N-(4-guanidinobenzyl)-D-ribitol
-
pH 7.0, 35°C
0.000029
1,4-dideoxy-1,4-imino-N-(4-methoxyquinolin-8-yl)methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.043
1,4-dideoxy-1,4-imino-N-(4-methyl-2-phenylpyrimidin-5-yl)methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.02
1,4-dideoxy-1,4-imino-N-(4-pyridinyl)methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.029
1,4-dideoxy-1,4-imino-N-(4-quinolinyl)methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.032
1,4-dideoxy-1,4-imino-N-(6-methoxypyridin-3-yl)methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.0023
1,4-dideoxy-1,4-imino-N-(7-methoxy-indol-3-yl)methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.00072
1,4-dideoxy-1,4-imino-N-(7-quinolinyl)methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.00002
1,4-dideoxy-1,4-imino-N-(7-thieno[3,2-d]pyrimidonyl)-methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.001
1,4-dideoxy-1,4-imino-N-(8-hydroxyquinolin-2-yl)methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.0000108
1,4-dideoxy-1,4-imino-N-(8-quinolinyl)methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.000058
1,4-dideoxy-1,4-imino-N-(quinazolin-8-yl)methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.000109
1,4-dideoxy-1,4-imino-N-(quinazolon-8-yl)methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.000139
1,4-dideoxy-1,4-imino-N-(quinoxalin-5-yl)-methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.000042
1,4-dideoxy-1,4-imino-N-(thieno[3,2-d]pyrimidin-7-yl)methyl-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.0034
1,4-dideoxy-1,4-imino-N-[3-(1H-pyrrol-1-yl)benzyl]-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.000063 - 0.0012
1,4-dideoxy-1,4-imino-N-[4-(1H-1,2,4-triazol-1-yl)benzyl]-D-ribitol
0.0012
1,4-dideoxy-1,4-imino-N-[4-(1H-pyrazol-1-yl)benzyl]-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.001
1,4-dideoxy-1,4-imino-N-[4-(1H-pyrrol-1-yl)benzyl]-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.545
1-guanidino-2,5-anhydro-1-deoxy-D-allitol
-
pH 7.0, 35°C
0.309
1-guanidino-3,6-anhydro-1,2-dideoxy-D-allo-heptitol
-
pH 7.0, 35°C
0.00000048 - 0.0000063
1-[(7-benzyloxymethyl-6-tert-butoxy-9-deazapurin-9-yl)methyl] azetidine-3-methanol
0.000000229 - 0.000000665
1-[(9-deazahypoxanthin-9-yl)methyl]azetidine-3,3dimethanol
0.018
1-[2-[(2S,3S,4R,5R)-3,4-dihydroxy-5-(hydroxymethyl)pyrrolidin-2-yl]ethyl]guanidine
-
0.04
1-[3-[(2R,3R,4S)-3,4-dihydroxy-2-(hydroxymethyl)pyrrolidin-1-yl]propyl]guanidine
-
0.182
2'-deoxyadenosine
-
at pH 7.0 and 35°C
0.18
3'-deoxyadenosine
-
at pH 7.0 and 35°C
0.0002
3-deazaadenosine
pH 7.2, 35°C, His-tagged enzyme form
0.000001 - 0.0000011
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin H
0.3565
7-deazaadenosine
pH 7.2, 35°C, His-tagged enzyme form
0.0000044
7-[[(2R,3R,4S)-3,4-dihydroxy-2-(hydroxymethyl)pyrrolidin-1-yl]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.000015
8-aza-immucillin-H
-
at pH 7.0 and 35°C
0.0009
Cu2+
-
noncompetitive, recombinant enzyme, pH 7.3, 37°C
8.74
D-ribose
-
pH 7.2, 37°C
0.0038
daidzein
-
pH 7.3, 37°C
0.011
formononetin
-
pH 7.3, 37°C
0.0000009 - 6.2
immucillin A
0.0000062 - 6.2
immucillin H
0.000009
immucillin-A
-
at pH 7.0 and 35°C
0.000044
immucillin-H
-
at pH 7.0 and 35°C
0.0000129 - 0.000016
meso-[(9-deazahypoxanthin-9-yl)methyl]azetidine-2,4-dimethanol hydrochloride
0.0000041 - 0.59
N-(9-deaza-adenin-9-yl)methyl-1,4-dideoxy-1,4-imino-D-ribitol
0.0000044
N-(9-deazahypoxanthin-9-yl)methyl-1,4-dideoxy-1,4-imino-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.059
N-benzyl-1,4-dideoxy-1,4-imino-D-ribitol
-
pH 7.0, 35°C
0.0000044 - 0.138
UAMC-00109
0.000011 - 0.011
UAMC-00115
0.00004
UAMC-00218
-
pH and temperature not specified in the publication
0.00004 - 0.014
UAMC-00311
0.00002 - 0.12
UAMC-00312
0.000058
UAMC-00346
-
pH and temperature not specified in the publication
0.0000041 - 0.032
UAMC-00363
0.000019 - 0.031
UAMC-00375
0.0000018
(+-)-1-[(9-deazahypoxanthin-9-yl)methyl]azetidine-2-methanol
-
pH 7.0
0.0000018
(+-)-1-[(9-deazahypoxanthin-9-yl)methyl]azetidine-2-methanol
-
pH 7.0
0.000063
1,4-dideoxy-1,4-imino-N-[4-(1H-1,2,4-triazol-1-yl)benzyl]-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.0012
1,4-dideoxy-1,4-imino-N-[4-(1H-1,2,4-triazol-1-yl)benzyl]-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.00000048
1-[(7-benzyloxymethyl-6-tert-butoxy-9-deazapurin-9-yl)methyl] azetidine-3-methanol
-
pH 7.0
0.0000063
1-[(7-benzyloxymethyl-6-tert-butoxy-9-deazapurin-9-yl)methyl] azetidine-3-methanol
-
pH 7.0
0.000000229
1-[(9-deazahypoxanthin-9-yl)methyl]azetidine-3,3dimethanol
-
pH 7.0
0.000000665
1-[(9-deazahypoxanthin-9-yl)methyl]azetidine-3,3dimethanol
-
pH 7.0
0.000001
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin H
-
pH 7.0
0.0000011
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin H
-
pH 7.0
1.2
8-aza-immucillin H
-
mutant R252A, pH 7.0, 35°C
1.2
8-aza-immucillin H
-
wild-type, pH 7.0, 35°C
0.16
Co2+
-
competitive, recombinant enzyme, pH 7.3, 37°C
1
Co2+
-
noncompetitive, recombinant enzyme, pH 7.3, 37°C
0.00211
cytidine
E15229
pH 7.0, 30°C
0.00415
cytidine
E15229
pH 7.0, 30°C
0.0112
cytidine
E15229
pH 7.0, 30°C
0.016
cytidine
E15229
pH 7.0, 30°C
0.0000009
immucillin A
-
inosine-adenosine-guanosine nucleoside hydrolase, pH and temperature not specified in the publication
0.0000044
immucillin A
-
inosine-guanosine nucleoside hydrolase, pH and temperature not specified in the publication
0.0000062
immucillin A
-
pH and temperature not specified in the publication
6.2
immucillin A
-
mutant R252A, pH 7.0, 35°C
6.2
immucillin A
-
wild-type, pH 7.0, 35°C
0.0000062
immucillin H
-
0.0000062
immucillin H
-
pH and temperature not specified in the publication
0.000009
immucillin H
-
inosine-guanosine nucleoside hydrolase, pH and temperature not specified in the publication
0.000024
immucillin H
-
inosine-adenosine-guanosine nucleoside hydrolase, pH and temperature not specified in the publication
6.2
immucillin H
-
mutant R252A, pH 7.0, 35°C
6.2
immucillin H
-
wild-type, pH 7.0, 35°C
0.0000129
meso-[(9-deazahypoxanthin-9-yl)methyl]azetidine-2,4-dimethanol hydrochloride
-
pH 7.0
0.000016
meso-[(9-deazahypoxanthin-9-yl)methyl]azetidine-2,4-dimethanol hydrochloride
-
pH 7.0
0.29
Mn2+
-
competitive, recombinant enzyme, pH 7.3, 37°C
1
Mn2+
-
noncompetitive, recombinant enzyme, pH 7.3, 37°C
0.0000041
N-(9-deaza-adenin-9-yl)methyl-1,4-dideoxy-1,4-imino-D-ribitol
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.59
N-(9-deaza-adenin-9-yl)methyl-1,4-dideoxy-1,4-imino-D-ribitol
-
-
0.028
Ni2+
-
competitive, recombinant enzyme, pH 7.3, 37°C
0.39
Ni2+
-
noncompetitive, recombinant enzyme, pH 7.3, 37°C
0.0000044
UAMC-00109
-
pH and temperature not specified in the publication
0.00003
UAMC-00109
-
inosine-adenosine-guanosine nucleoside hydrolase, pH and temperature not specified in the publication
0.138
UAMC-00109
-
inosine-guanosine nucleoside hydrolase, pH and temperature not specified in the publication
0.000011
UAMC-00115
-
pH and temperature not specified in the publication
0.000446
UAMC-00115
-
inosine-adenosine-guanosine nucleoside hydrolase, pH and temperature not specified in the publication
0.011
UAMC-00115
-
inosine-guanosine nucleoside hydrolase, pH and temperature not specified in the publication
0.00004
UAMC-00311
-
pH and temperature not specified in the publication
0.00056
UAMC-00311
-
inosine-adenosine-guanosine nucleoside hydrolase, pH and temperature not specified in the publication
0.014
UAMC-00311
-
inosine-guanosine nucleoside hydrolase, pH and temperature not specified in the publication
0.00002
UAMC-00312
-
pH and temperature not specified in the publication
0.00019
UAMC-00312
-
inosine-adenosine-guanosine nucleoside hydrolase, pH and temperature not specified in the publication
0.12
UAMC-00312
-
inosine-guanosine nucleoside hydrolase, pH and temperature not specified in the publication
0.0000041
UAMC-00363
-
pH and temperature not specified in the publication
0.000018
UAMC-00363
-
inosine-adenosine-guanosine nucleoside hydrolase, pH and temperature not specified in the publication
0.032
UAMC-00363
-
inosine-guanosine nucleoside hydrolase, pH and temperature not specified in the publication
0.000019
UAMC-00375
-
pH and temperature not specified in the publication
0.000056
UAMC-00375
-
inosine-adenosine-guanosine nucleoside hydrolase, pH and temperature not specified in the publication
0.031
UAMC-00375
-
inosine-guanosine nucleoside hydrolase, pH and temperature not specified in the publication
0.0028
Zn2+
-
competitive, recombinant enzyme, pH 7.3, 37°C
0.0167
Zn2+
-
noncompetitive, recombinant enzyme, pH 7.3, 37°C
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