Any feedback?
Literature summary for 3.2.2.1 extracted from
- Purine-specific nucleoside N-ribohydrolase from Trypanosoma brucei brucei (1996), J. Biol. Chem., 271, 21713-21719.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-deazaadenosine | - |
Trypanosoma brucei |  |
| 7-deazaadenosine | i.e. tubercidin | Trypanosoma brucei | |
| D-ribose | - |
Trypanosoma brucei | |
| formycin B | - |
Trypanosoma brucei | |
| hypoxanthine | - |
Trypanosoma brucei | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
adenosine | - |
Trypanosoma brucei | |
| 0.018 | - |
Inosine | - |
Trypanosoma brucei | |
| 0.019 | - |
purine riboside | - |
Trypanosoma brucei | |
| 0.026 | - |
5-methyluridine | - |
Trypanosoma brucei | |
| 0.042 | - |
2'-deoxyadenosine | - |
Trypanosoma brucei | |
| 0.046 | - |
guanosine | - |
Trypanosoma brucei | |
| 0.102 | - |
uridine | - |
Trypanosoma brucei | |
| 0.106 | - |
cytidine | - |
Trypanosoma brucei | |
| 0.562 | - |
p-nitrophenylriboside | - |
Trypanosoma brucei | |
| 0.6 | - |
3'-deoxyadenosine | - |
Trypanosoma brucei | |
| 9.87 | - |
5'-deoxyadenosine | - |
Trypanosoma brucei | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35759 | - |
2 * 35759, electrospray ionization mass spectrometry | Trypanosoma brucei |
| 41000 | - |
2 * 41000, SDS-PAGE | Trypanosoma brucei |
| 71000 | - |
gel filtration | Trypanosoma brucei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma brucei | - |
brucei | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Trypanosoma brucei |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bloodstream form | - |
Trypanosoma brucei | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 50 | - |
hydrolysis of inosine | Trypanosoma brucei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-deoxyadenosine + H2O | - |
Trypanosoma brucei | D-ribose + ? | - |
? | |
| 3'-deoxyadenosine + H2O | - |
Trypanosoma brucei | D-ribose + ? | - |
? | |
| 5'-deoxyadenosine + H2O | - |
Trypanosoma brucei | D-ribose + ? | - |
? | |
| 5-methyluridine + H2O | - |
Trypanosoma brucei | 5-methyluracil + D-ribose | - |
? | |
| adenosine + H2O | - |
Trypanosoma brucei | D-ribose + adenine | - |
? | |
| cytidine + H2O | - |
Trypanosoma brucei | cytosine + D-ribose | - |
r | |
| guanosine + H2O | - |
Trypanosoma brucei | D-ribose + guanine | - |
? | |
| inosine + H2O | kinetic mechanism is rapid equilibrium with random product release | Trypanosoma brucei | hypoxanthine + D-ribose | - |
? | |
| p-nitrophenylriboside + H2O | - |
Trypanosoma brucei | 4-nitrophenol + D-ribose | - |
? | |
| purine riboside + H2O | - |
Trypanosoma brucei | purine + D-ribose | - |
? | |
| uridine + H2O | - |
Trypanosoma brucei | D-ribose + uracil | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 41000, SDS-PAGE | Trypanosoma brucei |
| dimer | 2 * 35759, electrospray ionization mass spectrometry | Trypanosoma brucei |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 34 | - |
Inosine | - |
Trypanosoma brucei | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Trypanosoma brucei |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | stable | Trypanosoma brucei |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
