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Literature summary for 3.2.2.1 extracted from
- Cloning, preliminary characterization and crystallization of nucleoside hydrolases from Caenorhabditis elegans and Campylobacter jejuni (2003), Acta Crystallogr. Sect. D, D59, 1087-1089.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Caenorhabditis elegans |
| expression in Escherichia coli | Campylobacter jejuni |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging-drop vapour-diffusion method | Caenorhabditis elegans |
| hanging-drop vapour-diffusion method | Campylobacter jejuni |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.093 | - |
adenosine | - |
Caenorhabditis elegans |  |
| 0.164 | - |
guanosine | - |
Caenorhabditis elegans | |
| 0.295 | - |
Inosine | - |
Caenorhabditis elegans | |
| 1.038 | - |
Xanthosine | - |
Caenorhabditis elegans | |
| 2.84 | - |
uridine | - |
Caenorhabditis elegans | |
| 10 | - |
cytidine | - |
Caenorhabditis elegans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
4 * 38000 | Caenorhabditis elegans |
| 39000 | - |
4 * 39000 | Campylobacter jejuni |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caenorhabditis elegans | - |
- |
- |
| Campylobacter jejuni | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Caenorhabditis elegans |
- |
Campylobacter jejuni |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenosine + H2O | - |
Caenorhabditis elegans | adenine + D-ribose | - |
? | |
| adenosine + H2O | - |
Campylobacter jejuni | adenine + D-ribose | - |
? | |
| cytidine + H2O | - |
Caenorhabditis elegans | cytosine + D-ribose | - |
? | |
| cytidine + H2O | - |
Campylobacter jejuni | cytosine + D-ribose | - |
? | |
| guanosine + H2O | - |
Caenorhabditis elegans | guanine + D-ribose | - |
? | |
| guanosine + H2O | - |
Campylobacter jejuni | guanine + D-ribose | - |
? | |
| inosine + H2O | - |
Caenorhabditis elegans | hypoxanthine + D-ribose | - |
? | |
| inosine + H2O | - |
Campylobacter jejuni | hypoxanthine + D-ribose | - |
? | |
| uridine + H2O | - |
Caenorhabditis elegans | uracil + D-ribose | - |
? | |
| uridine + H2O | - |
Campylobacter jejuni | uracil + D-ribose | - |
? | |
| xanthosine + H2O | - |
Caenorhabditis elegans | xanthine + D-ribose | - |
? | |
| xanthosine + H2O | - |
Campylobacter jejuni | xanthine + D-ribose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 38000 | Caenorhabditis elegans |
| tetramer | 4 * 39000 | Campylobacter jejuni |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0022 | - |
uridine | - |
Caenorhabditis elegans | |
| 0.029 | - |
Xanthosine | - |
Caenorhabditis elegans | |
| 0.5 | - |
cytidine | - |
Caenorhabditis elegans | |
| 0.78 | - |
adenosine | - |
Caenorhabditis elegans | |
| 0.79 | - |
Inosine | - |
Caenorhabditis elegans | |
| 0.84 | - |
guanosine | - |
Caenorhabditis elegans | |
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