Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta(DE3) cells | Trypanosoma brucei brucei |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 100 mM Bis-Tris (pH 6.5), 200 mM ammonium sulfate, and 22% (w/v) polyethylene glycol monomethyl ether 3350 | Trypanosoma brucei brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2'-deoxyadenosine | - |
Trypanosoma brucei brucei | |
3'-deoxyadenosine | - |
Trypanosoma brucei brucei | |
8-aza-immucillin-H | nanomolar inhibitor | Trypanosoma brucei brucei | |
immucillin-A | nanomolar inhibitor | Trypanosoma brucei brucei | |
immucillin-H | nanomolar inhibitor | Trypanosoma brucei brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
purine riboside | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
0.0008 | - |
2-aminopurine riboside | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
0.001 | - |
adenosine | Km less than 0.001 mM, at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
0.0011 | - |
4-nitrophenyl beta-D-ribofuranoside | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
0.0019 | - |
Inosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
0.0022 | - |
3-deazaadenosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
0.0036 | - |
ethenoadenosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
0.0045 | - |
guanosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
0.24 | - |
5'-deoxyadenosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
0.271 | - |
cytidine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
0.28 | - |
Xanthosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
1.451 | - |
uridine | at pH 7.0 and 35°C | Trypanosoma brucei brucei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanosine + H2O | Trypanosoma brucei brucei | - |
D-ribose + guanine | - |
? | |
inosine + H2O | Trypanosoma brucei brucei | - |
D-ribose + hypoxanthine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei brucei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography and Superdex 200 gel filtration | Trypanosoma brucei brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminopurine riboside + H2O | - |
Trypanosoma brucei brucei | 2-aminopurine + D-ribose | - |
? | |
3-deazaadenosine + H2O | - |
Trypanosoma brucei brucei | D-ribose + 3-deazaadenine | - |
? | |
4-nitrophenyl beta-D-ribofuranoside + H2O | - |
Trypanosoma brucei brucei | 4-nitrophenol + beta-D-ribofuranose | - |
? | |
5'-deoxyadenosine + H2O | - |
Trypanosoma brucei brucei | 5-deoxy-D-ribose + adenine | - |
? | |
7-methylguanosine + H2O | - |
Trypanosoma brucei brucei | ? | - |
? | |
adenosine + H2O | - |
Trypanosoma brucei brucei | D-ribose + adenine | - |
? | |
cytidine + H2O | - |
Trypanosoma brucei brucei | D-ribose + cytosine | - |
? | |
ethenoadenosine + H2O | - |
Trypanosoma brucei brucei | D-ribose + ethenoadenine | - |
? | |
guanosine + H2O | - |
Trypanosoma brucei brucei | D-ribose + guanine | - |
? | |
guanosine + H2O | preferred substrate | Trypanosoma brucei brucei | D-ribose + guanine | - |
? | |
inosine + H2O | - |
Trypanosoma brucei brucei | D-ribose + hypoxanthine | - |
? | |
inosine + H2O | preferred substrate | Trypanosoma brucei brucei | D-ribose + hypoxanthine | - |
? | |
purine riboside + H2O | - |
Trypanosoma brucei brucei | purine + D-ribose | - |
? | |
uridine + H2O | - |
Trypanosoma brucei brucei | D-ribose + uracil | - |
? | |
xanthosine + H2O | - |
Trypanosoma brucei brucei | D-ribose + xanthine | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | x-ray crystallography | Trypanosoma brucei brucei |
Synonyms | Comment | Organism |
---|---|---|
6-oxopurine nucleosidase | - |
Trypanosoma brucei brucei |
IG-NH | - |
Trypanosoma brucei brucei |
inosine-guanosine-specific nucleoside hydrolase | - |
Trypanosoma brucei brucei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0038 | - |
uridine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
0.009 | - |
3-deazaadenosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
0.03 | - |
4-nitrophenyl beta-D-ribofuranoside | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
0.078 | - |
5'-deoxyadenosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
0.237 | - |
adenosine | Km less than 0.001 mM, at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
0.248 | - |
purine riboside | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
0.52 | - |
cytidine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
0.57 | - |
2-aminopurine riboside | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
1.3 | - |
guanosine | at pH 7.0 and 5°C | Trypanosoma brucei brucei | |
2 | 8 | Inosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
5.2 | - |
7-methylguanosine | at pH 7.0 and 5°C | Trypanosoma brucei brucei | |
6.2 | - |
Xanthosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
6.9 | - |
ethenoadenosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
37.6 | - |
guanosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000009 | - |
immucillin-A | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
0.000015 | - |
8-aza-immucillin-H | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
0.000044 | - |
immucillin-H | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
0.18 | - |
3'-deoxyadenosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
0.182 | - |
2'-deoxyadenosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0026 | - |
uridine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
0.33 | - |
5'-deoxyadenosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
1.9 | - |
cytidine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
22 | - |
Xanthosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
27 | - |
4-nitrophenyl beta-D-ribofuranoside | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
41 | - |
3-deazaadenosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
240 | - |
adenosine | kcat_Km above 240/sec*mM, at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
380 | - |
purine riboside | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
700 | - |
2-aminopurine riboside | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
1900 | - |
ethenoadenosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
8300 | - |
guanosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei | |
15000 | - |
Inosine | at pH 7.0 and 35°C | Trypanosoma brucei brucei |