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Literature summary for 3.2.2.1 extracted from

  • Vandemeulebroucke, A.; Minici, C.; Bruno, I.; Muzzolini, L.; Tornaghi, P.; Parkin, D.W.; Versees, W.; Steyaert, J.; Degano, M.
    Structure and mechanism of the 6-oxopurine nucleosidase from Trypanosoma brucei brucei (2010), Biochemistry, 49, 8999-9010.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta(DE3) cells Trypanosoma brucei brucei

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 100 mM Bis-Tris (pH 6.5), 200 mM ammonium sulfate, and 22% (w/v) polyethylene glycol monomethyl ether 3350 Trypanosoma brucei brucei

Inhibitors

Inhibitors Comment Organism Structure
2'-deoxyadenosine
-
Trypanosoma brucei brucei
3'-deoxyadenosine
-
Trypanosoma brucei brucei
8-aza-immucillin-H nanomolar inhibitor Trypanosoma brucei brucei
immucillin-A nanomolar inhibitor Trypanosoma brucei brucei
immucillin-H nanomolar inhibitor Trypanosoma brucei brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0006
-
purine riboside at pH 7.0 and 35°C Trypanosoma brucei brucei
0.0008
-
2-aminopurine riboside at pH 7.0 and 35°C Trypanosoma brucei brucei
0.001
-
adenosine Km less than 0.001 mM, at pH 7.0 and 35°C Trypanosoma brucei brucei
0.0011
-
4-nitrophenyl beta-D-ribofuranoside at pH 7.0 and 35°C Trypanosoma brucei brucei
0.0019
-
Inosine at pH 7.0 and 35°C Trypanosoma brucei brucei
0.0022
-
3-deazaadenosine at pH 7.0 and 35°C Trypanosoma brucei brucei
0.0036
-
ethenoadenosine at pH 7.0 and 35°C Trypanosoma brucei brucei
0.0045
-
guanosine at pH 7.0 and 35°C Trypanosoma brucei brucei
0.24
-
5'-deoxyadenosine at pH 7.0 and 35°C Trypanosoma brucei brucei
0.271
-
cytidine at pH 7.0 and 35°C Trypanosoma brucei brucei
0.28
-
Xanthosine at pH 7.0 and 35°C Trypanosoma brucei brucei
1.451
-
uridine at pH 7.0 and 35°C Trypanosoma brucei brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
guanosine + H2O Trypanosoma brucei brucei
-
D-ribose + guanine
-
?
inosine + H2O Trypanosoma brucei brucei
-
D-ribose + hypoxanthine
-
?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei brucei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography and Superdex 200 gel filtration Trypanosoma brucei brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminopurine riboside + H2O
-
Trypanosoma brucei brucei 2-aminopurine + D-ribose
-
?
3-deazaadenosine + H2O
-
Trypanosoma brucei brucei D-ribose + 3-deazaadenine
-
?
4-nitrophenyl beta-D-ribofuranoside + H2O
-
Trypanosoma brucei brucei 4-nitrophenol + beta-D-ribofuranose
-
?
5'-deoxyadenosine + H2O
-
Trypanosoma brucei brucei 5-deoxy-D-ribose + adenine
-
?
7-methylguanosine + H2O
-
Trypanosoma brucei brucei ?
-
?
adenosine + H2O
-
Trypanosoma brucei brucei D-ribose + adenine
-
?
cytidine + H2O
-
Trypanosoma brucei brucei D-ribose + cytosine
-
?
ethenoadenosine + H2O
-
Trypanosoma brucei brucei D-ribose + ethenoadenine
-
?
guanosine + H2O
-
Trypanosoma brucei brucei D-ribose + guanine
-
?
guanosine + H2O preferred substrate Trypanosoma brucei brucei D-ribose + guanine
-
?
inosine + H2O
-
Trypanosoma brucei brucei D-ribose + hypoxanthine
-
?
inosine + H2O preferred substrate Trypanosoma brucei brucei D-ribose + hypoxanthine
-
?
purine riboside + H2O
-
Trypanosoma brucei brucei purine + D-ribose
-
?
uridine + H2O
-
Trypanosoma brucei brucei D-ribose + uracil
-
?
xanthosine + H2O
-
Trypanosoma brucei brucei D-ribose + xanthine
-
?

Subunits

Subunits Comment Organism
homotetramer x-ray crystallography Trypanosoma brucei brucei

Synonyms

Synonyms Comment Organism
6-oxopurine nucleosidase
-
Trypanosoma brucei brucei
IG-NH
-
Trypanosoma brucei brucei
inosine-guanosine-specific nucleoside hydrolase
-
Trypanosoma brucei brucei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0038
-
uridine at pH 7.0 and 35°C Trypanosoma brucei brucei
0.009
-
3-deazaadenosine at pH 7.0 and 35°C Trypanosoma brucei brucei
0.03
-
4-nitrophenyl beta-D-ribofuranoside at pH 7.0 and 35°C Trypanosoma brucei brucei
0.078
-
5'-deoxyadenosine at pH 7.0 and 35°C Trypanosoma brucei brucei
0.237
-
adenosine Km less than 0.001 mM, at pH 7.0 and 35°C Trypanosoma brucei brucei
0.248
-
purine riboside at pH 7.0 and 35°C Trypanosoma brucei brucei
0.52
-
cytidine at pH 7.0 and 35°C Trypanosoma brucei brucei
0.57
-
2-aminopurine riboside at pH 7.0 and 35°C Trypanosoma brucei brucei
1.3
-
guanosine at pH 7.0 and 5°C Trypanosoma brucei brucei
2 8 Inosine at pH 7.0 and 35°C Trypanosoma brucei brucei
5.2
-
7-methylguanosine at pH 7.0 and 5°C Trypanosoma brucei brucei
6.2
-
Xanthosine at pH 7.0 and 35°C Trypanosoma brucei brucei
6.9
-
ethenoadenosine at pH 7.0 and 35°C Trypanosoma brucei brucei
37.6
-
guanosine at pH 7.0 and 35°C Trypanosoma brucei brucei

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000009
-
immucillin-A at pH 7.0 and 35°C Trypanosoma brucei brucei
0.000015
-
8-aza-immucillin-H at pH 7.0 and 35°C Trypanosoma brucei brucei
0.000044
-
immucillin-H at pH 7.0 and 35°C Trypanosoma brucei brucei
0.18
-
3'-deoxyadenosine at pH 7.0 and 35°C Trypanosoma brucei brucei
0.182
-
2'-deoxyadenosine at pH 7.0 and 35°C Trypanosoma brucei brucei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0026
-
uridine at pH 7.0 and 35°C Trypanosoma brucei brucei
0.33
-
5'-deoxyadenosine at pH 7.0 and 35°C Trypanosoma brucei brucei
1.9
-
cytidine at pH 7.0 and 35°C Trypanosoma brucei brucei
22
-
Xanthosine at pH 7.0 and 35°C Trypanosoma brucei brucei
27
-
4-nitrophenyl beta-D-ribofuranoside at pH 7.0 and 35°C Trypanosoma brucei brucei
41
-
3-deazaadenosine at pH 7.0 and 35°C Trypanosoma brucei brucei
240
-
adenosine kcat_Km above 240/sec*mM, at pH 7.0 and 35°C Trypanosoma brucei brucei
380
-
purine riboside at pH 7.0 and 35°C Trypanosoma brucei brucei
700
-
2-aminopurine riboside at pH 7.0 and 35°C Trypanosoma brucei brucei
1900
-
ethenoadenosine at pH 7.0 and 35°C Trypanosoma brucei brucei
8300
-
guanosine at pH 7.0 and 35°C Trypanosoma brucei brucei
15000
-
Inosine at pH 7.0 and 35°C Trypanosoma brucei brucei