Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.2.1 extracted from

  • Hansen, M.R.; Dandanell, G.
    Purification and characterization of RihC, a xanthosine-inosine-uridine-adenosine-preferring hydrolase from Salmonella enterica serovar Typhimurium (2005), Biochim. Biophys. Acta, 1723, 55-62.
    View publication on PubMed
Search for more literature for 3.2.2.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Salmonella enterica

Inhibitors

Inhibitors Comment Organism Structure
D-ribose product inhibition Salmonella enterica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
 adenosine pH 7.2, 37°C Salmonella enterica
0.65
-
 Inosine pH 7.2, 37°C Salmonella enterica
0.79
-
 Xanthosine pH 6.0, 37°C Salmonella enterica
1.06
-
 uridine pH 7.2, 37°C Salmonella enterica
1.28
-
 Inosine pH 6.0, 37°C Salmonella enterica
5.9
-
 Xanthosine pH 7.2, 37°C Salmonella enterica
9.2
-
 cytidine pH 7.2, 37°C Salmonella enterica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33100
-
 4 * 33100, calculated Salmonella enterica
128000
-
 sucrose density gradient centrifugation Salmonella enterica
142000
-
 gel filtration Salmonella enterica

Organism

Organism UniProt Comment Textmining
Salmonella enterica
-
 serovar typhimurium
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.3
-
 pH 7.2, 37°C Salmonella enterica

Storage Stability

Storage Stability Organism
4°C, stable for at least 6 months Salmonella enterica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine + H2O
-
 Salmonella enterica adenine + D-ribose
-
 ?
cytidine + H2O
-
 Salmonella enterica D-ribose + cytosine
-
 ?
guanosine + H2O
-
 Salmonella enterica D-ribose + guanine
-
 ?
inosine + H2O
-
 Salmonella enterica hypoxanthine + D-ribose
-
 ?
uridine + H2O
-
 Salmonella enterica D-ribose + uracil
-
 ?
xanthosine + H2O
-
 Salmonella enterica xanthine + D-ribose
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 33100, calculated Salmonella enterica

Synonyms

Synonyms Comment Organism
RihC
-
 Salmonella enterica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 12 h, no loss of activity Salmonella enterica
60
-
 10 min, no loss of activity Salmonella enterica
70
-
 10 min, no loss of activity Salmonella enterica
80
-
 half-life 5.9 min Salmonella enterica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1
-
 adenosine pH 7.2, 37°C Salmonella enterica
7.8
-
 cytidine pH 7.2, 37°C Salmonella enterica
8.1
-
 Inosine pH 6.0, 37°C Salmonella enterica
9
-
 Inosine pH 7.2, 37°C Salmonella enterica
26.3
-
 Xanthosine pH 6.0, 37°C Salmonella enterica
46
-
 uridine pH 7.2, 37°C Salmonella enterica
62
-
 Xanthosine pH 7.2, 37°C Salmonella enterica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6
-
 substrate xanthosine Salmonella enterica
7.8
-
 substrate inosine Salmonella enterica

pH Range

pH Minimum pH Maximum Comment Organism
9
-
 substrate inosine, more than 50% of maximum activity Salmonella enterica

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
8.74
-
 D-ribose pH 7.2, 37°C Salmonella enterica