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Literature summary for 3.2.2.1 extracted from
- Nucleoside hydrolase from Crithidia fasciculata. Metabolic role, purification, specificity, and kinetic mechanism (1991), J. Biol. Chem., 266, 20658-20665.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 7-deazaadenosine | i.e. tubercidin | Crithidia fasciculata |  |
| D-ribose | product inhibition | Crithidia fasciculata | |
| hypoxanthine | product inhibition | Crithidia fasciculata | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.145 | - |
purine riboside | - |
Crithidia fasciculata | |
| 0.38 | - |
Inosine | - |
Crithidia fasciculata | |
| 0.42 | - |
guanosine | - |
Crithidia fasciculata | |
| 0.46 | - |
adenosine | - |
Crithidia fasciculata | |
| 1.22 | - |
uridine | - |
Crithidia fasciculata | |
| 1.3 | - |
5-methyluridine | - |
Crithidia fasciculata | |
| 4.7 | - |
cytosine | - |
Crithidia fasciculata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
4 * 34000, SDS-PAGE | Crithidia fasciculata |
| 143000 | - |
gel filtration | Crithidia fasciculata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Crithidia fasciculata | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Crithidia fasciculata |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.047 | - |
- |
Crithidia fasciculata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-methyluridine + H2O | - |
Crithidia fasciculata | 5-methyluracil + D-ribose | - |
? | |
| adenosine + H2O | - |
Crithidia fasciculata | D-ribose + adenine | - |
? | |
| cytosine + D-ribose | - |
Crithidia fasciculata | cytidine + H2O | - |
r | |
| guanosine + H2O | - |
Crithidia fasciculata | D-ribose + guanine | - |
? | |
| inosine + H2O | kinetic mechanism is rapid equilibrium with random product release | Crithidia fasciculata | hypoxanthine + D-ribose | - |
? | |
| purine ribonucleoside + H2O | - |
Crithidia fasciculata | purine + D-ribose | - |
? | |
| purine riboside + H2O | - |
Crithidia fasciculata | purine + D-ribose | - |
? | |
| uridine + H2O | - |
Crithidia fasciculata | D-ribose + uracil | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 34000, SDS-PAGE | Crithidia fasciculata |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 32 | - |
Inosine | - |
Crithidia fasciculata | |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.7 | stable | Crithidia fasciculata |
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