Any feedback?
Literature summary for 3.2.2.1 extracted from
- Purification and properties of purine nucleosidase from N2-fixing nodules of cowpea (Vigna unguiculata) (1989), J. Plant Physiol., 134, 447-452.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| adenosine | competitive inhibitor of xanthosine hydrolysis and inosine hydrolysis | Vigna unguiculata |  |
| guanosine | competitive inhibitor of xanthosine hydrolysis | Vigna unguiculata | |
| Inosine | competitive inhibitor of xanthosine hydrolysis | Vigna unguiculata | |
| uridine | competitive inhibitor of xanthosine hydrolysis | Vigna unguiculata | |
| Xanthosine | competitive inhibitor of inosine hydrolysis | Vigna unguiculata | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
Xanthosine | - |
Vigna unguiculata | |
| 0.83 | - |
Inosine | - |
Vigna unguiculata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30600 | - |
5 * 30600, SDS-PAGE | Vigna unguiculata |
| 160000 | - |
gel filtration | Vigna unguiculata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vigna unguiculata | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Vigna unguiculata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| nodule | N2-fixing, nodulated with Rhizobium strain B 756 | Vigna unguiculata | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.432 | - |
- |
Vigna unguiculata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenosine + H2O | 3.6% of the activity with xanthosine | Vigna unguiculata | D-ribose + adenine | - |
? | |
| cytidine + H2O | - |
Vigna unguiculata | D-ribose + cytosine | - |
? | |
| guanosine + H2O | 1.4% of the activity with xanthosine | Vigna unguiculata | D-ribose + guanine | - |
? | |
| inosine + H2O | 25% of the activity with xanthosine | Vigna unguiculata | hypoxanthine + D-ribose | - |
? | |
| thymidine + H2O | - |
Vigna unguiculata | D-ribose + thymine | - |
? | |
| uridine + H2O | - |
Vigna unguiculata | D-ribose + uracil | - |
? | |
| xanthosine + H2O | - |
Vigna unguiculata | D-ribose + xanthine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| pentamer | 5 * 30600, SDS-PAGE | Vigna unguiculata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | hydrolysis of inosine and xanthosine | Vigna unguiculata |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
