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Literature summary for 3.2.2.1 extracted from

  • Riegler, H.; Geserick, C.; Zrenner, R.
    Arabidopsis thaliana nucleosidase mutants provide new insights into nucleoside degradation (2011), New Phytol., 191, 349-359.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Arabidopsis thaliana 5829
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
 ecotype Columbia-0
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Arabidopsis thaliana
-
root
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosine + H2O
-
 Arabidopsis thaliana D-ribose + adenine
-
 ?
guanosine + H2O preferred substrate Arabidopsis thaliana D-ribose + guanine
-
 ?
inosine + H2O preferred substrate Arabidopsis thaliana D-ribose + hypoxanthine
-
 ?
uridine + H2O substrate for isoform URH1, but nor URH2 Arabidopsis thaliana D-ribose + uracil
-
 ?
xanthosine + H2O
-
 Arabidopsis thaliana D-ribose + xanthine
-
 ?

Synonyms

Synonyms Comment Organism
purine hydrolase
-
 Arabidopsis thaliana
URH1 isoform Arabidopsis thaliana
URH2 isoform Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function both isoforms URH1 and URH2 are unimportant for seedling establishment and plant growth, both isoform URH1 and URH2 are required for efficient inosine and xanthosine hydrolytic activity Arabidopsis thaliana