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Literature summary for 3.2.2.1 extracted from

  • Porcelli, M.; De Leo, E.; Marabotti, A.; Cacciapuoti, G.
    Site-directed mutagenesis gives insights into substrate specificity of Sulfolobus solfataricus purine-specific nucleoside hydrolase (2012), Ann. Microbiol., 62, 881-887.
    View publication on PubMed
Search for more literature for 3.2.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expression of mutant enzyme L221Y/N228V in Escherichia coli Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
L221Y/N228V site-directed mutagenesis, altered substrate specificity and cleavage pattern, as well as catalytic efficiency compared to the wild-type enzyme Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information a metalloenzyme Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97WH6
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-

Purification (Commentary)

Purification (Comment) Organism
recombinant mutant enzyme L221Y/N228V from Escherichia coli Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme catalyzes the irreversible hydrolysis of the N-glycosidic bond of beta-ribonucleosides forming ribose and purine base, substrate specificity and involved residues, overview Saccharolobus solfataricus ?
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 ?

Synonyms

Synonyms Comment Organism
purine-specific inosine-adenosine-guanosine nucleoside hydrolase
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 Saccharolobus solfataricus
purine-specific nucleoside hydrolase
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 Saccharolobus solfataricus
SslAG-NH
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 Saccharolobus solfataricus
SSO2243 locus name Saccharolobus solfataricus

General Information

General Information Comment Organism
additional information active site structure, homology modelling, overview Saccharolobus solfataricus