Information on EC 2.4.2.28 - S-methyl-5'-thioadenosine phosphorylase

Word Map on EC 2.4.2.28
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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY hide
2.4.2.28
-
RECOMMENDED NAME
GeneOntology No.
S-methyl-5'-thioadenosine phosphorylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pentosyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Cysteine and methionine metabolism
-
-
Metabolic pathways
-
-
S-methyl-5'-thioadenosine degradation II
-
-
polyamine pathway
-
-
SYSTEMATIC NAME
IUBMB Comments
S-methyl-5'-thioadenosine:phosphate S-methyl-5-thio-alpha-D-ribosyl-transferase
Also acts on 5'-deoxyadenosine and other analogues having 5'-deoxy groups.
CAS REGISTRY NUMBER
COMMENTARY hide
61970-06-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
Caldariella acidophila
-
-
-
Manually annotated by BRENDA team
O157:H7 EDL933
-
-
Manually annotated by BRENDA team
Wistar
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strains BB170 and BB120
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2',3'-dideoxyadenosine + phosphate
adenine + 2,3-deoxy-D-ribose 1-phosphate
show the reaction diagram
-
substrate for trypanosomal but not for mammalian enzyme
-
-
?
2'-chloro-2'-deoxyadenosine + phosphate
adenine + 2'-chloro-2'-deoxy-D-ribose 1-phosphate
show the reaction diagram
2'-deoxy-2'-amino-5'-deoxy-5'-methylthioadenosine + phosphate
adenine + 2-deoxy-2-amino-5-thio-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
2'-deoxy-2'-azido-5'-deoxy-5'-methylthioadenosine + phosphate
adenine + 2-deoxy-2-azido-5-thio-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
2'-deoxy-2-fluoro-5'-deoxy-5'-methylthioadenosine + phosphate
adenine + 2-deoxy-2-fluoro-5-thio-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
2'-deoxy-5'-deoxy-5'-methylthioadenosine + phosphate
adenine + 2-deoxy-5-thio-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
2'-deoxyadenosine + phosphate
adenine + 2-deoxy-D-ribose 1-phosphate
show the reaction diagram
2'-fluoro-2'-deoxyadenosine + phosphate
adenine + 2'-fluoro-2'-deoxy-D-ribose 1-phosphate
show the reaction diagram
2-amino-5'-deoxy-5'-(hydroxyethylthio)adenosine + phosphate
2-aminoadenosine + 5-hydroxyethylthio-D-ribose 1-phosphate
show the reaction diagram
-
28% activity compared to 5'-methyladenosine or 5'-deoxy-5'-(hydroxyethylthio)adenosine
-
-
?
2-chloro-2'-deoxyadenosine + phosphate
2-chloroadenine + 2-deoxy-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
r
2-chloro-5'-O-methyl-2'-deoxyadenosine + phosphate
2-chloroadenine + 5-O-methyl-2-deoxy-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
r
2-chloroadenosine + phosphate
2-chloroadenine + D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
r
2-fluoro-5'-deoxy-5'-(hydroxyethylthio)adenosine + phosphate
2-fluoroadenosine + 5-hydroxyethylthio-D-ribose 1-phosphate
show the reaction diagram
-
149% activity compared to 5'-methyladenosine or 5'-deoxy-5'-(hydroxyethylthio)adenosine
-
-
?
3'-deoxy-5'-deoxy-5'-methylthioadenosine + phosphate
adenine + 3-deoxy-5-thio-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
3'-deoxyadenosine + phosphate
adenine + 3-deoxy-D-ribose 1-phosphate
show the reaction diagram
-
substrate for trypanosomal but not for mammalian enzyme
-
-
?
5'-butylthioadenosine + phosphate
adenine + 5-butylthio-D-ribose 1-phosphate
show the reaction diagram
5'-chloroadenosine + phosphate
adenine + 5-deoxy-5-chloro-D-ribose 1-phosphate
show the reaction diagram
5'-deoxy-5'-(1,3-thiazol)-2-yl-sulfanyladenosine + phosphate
adenine + 5-(1,3-thiazol-2-yl)thio-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
5'-deoxy-5'-(2-(pyridin-2-yl)ethyl)-thioadenosine + phosphate
adenine + 5-ethylthio-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
5'-deoxy-5'-(2-methylbutyl)-thioadenosine + phosphate
adenine + 5-(2-methylbutyl)thio-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
5'-deoxy-5'-(2-methylphenyl)-thioadenosine + phosphate
adenine + 5-(2-methylphenyl)thio-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
5'-deoxy-5'-(3-methylphenyl)-thioadenosine + phosphate
adenine + 5-(3-methylphenyl)thio-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
5'-deoxy-5'-(4-methoxyphenyl)-thioadenosine + phosphate
adenine + 5-(4-methoxyphenyl)thio-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
5'-deoxy-5'-(4-methylphenyl)-thioadenosine + phosphate
adenine + 5-(4-methylphenyl)thio-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
5'-deoxy-5'-(hydroxyethylthio)adenosine + phosphate
adenine + 5-hydroxyethylthio-D-ribose 1-phosphate
show the reaction diagram
5'-deoxy-5'-(phenylethyl)-thioadenosine + phosphate
adenine + 5-phenylethylthio-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
5'-deoxy-5'-(phenylmethyl)-thioadenosine + phosphate
adenine + 5-phenylmethylthio-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
5'-deoxy-5'-(quinolin-2-yl)-sulfanyladenosine + phosphate
adenine + 5-(quinolin-2-yl)thio-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
5'-deoxy-5'-butylthioadenosine + phosphate
adenine + 5-butylthio-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
5'-deoxy-5'-cyclohexyl-thioadenosine + phosphate
adenine + 5-cyclohexylthio-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
5'-deoxy-5'-cyclopentyl-thioadenosine + phosphate
adenine + 5-cyclopentylthio-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
5'-deoxy-5'-ethylthioadenosine + phosphate
adenine + 5-ethylthio-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
5'-deoxy-5'-hydroxyethylthioadenosine + phosphate
adenine + 5-(2-hydroxyethyl)thio-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
5'-deoxy-5'-imidazol-2-yl-sulfanyladenosine + phosphate
adenine + 5-(imidazol-2-yl)thio-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
5'-deoxy-5'-isobutylthioadenosine + phosphate
adenine + 5-isobutylthio-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
5'-deoxy-5'-isopentylthioadenosine + phosphate
adenine + 5-isopentylthio-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
5'-deoxy-5'-methyl-thioadenosine + phosphate
adenine + 5-methylthio-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
5'-deoxy-5'-methylthioadenosine + phosphate
adenine + 5-methylthio-D-ribose 1-phosphate
show the reaction diagram
5'-deoxy-5'-phenyl-thioadenosine + phosphate
adenine + 5-phenylthio-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
5'-deoxy-5'-phenylmethylene-adenosine + phosphate
adenine + 5-deoxy-5-phenylmethylene-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
5'-deoxy-5'-pyridin-2-yl-sulfanyladenosine + phosphate
adenine + 5-(pyridin-2-yl)thio-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
5'-deoxy-5'-pyridin-4-yl-sulfanyladenosine + phosphate
adenine + 5-(pyridin-4-yl)thio-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
5'-deoxy-5'-tert-butylthioadenosine + phosphate
adenine + 5-tert-butylthio-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
5'-deoxy-5'-[4-(trifluoromethyl)phenyl]-thioadenosine + phosphate
adenine + 5-[4-(trifluoromethyl)phenyl]thio-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
5'-deoxy-5'phenylaminoadenosine + phosphate
adenine + 5-deoxy-5-phenylamino-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
5'-deoxy-adenosine + phosphate
adenine + 5-thio-D-ribose 1 phosphate
show the reaction diagram
-
-
-
-
?
5'-deoxyadenosine + phosphate
adenine + 5-deoxy-D-ribose 1-phosphate
show the reaction diagram
5'-ethylthioadenosine + phosphate
adenine + 5-ethylthio-D-ribose 1-phosphate
show the reaction diagram
5'-iodo-5'-deoxyadenosine + phosphate
adenine + 5-iodo-5-deoxy-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
r
5'-iodoadenosine + phosphate
adenine + 5-deoxy-5-iodo-D-ribose 1-phosphate
show the reaction diagram
5'-isobutylthioadenosine + phosphate
adenine + 5-isobutylthio-D-ribose 1-phosphate
show the reaction diagram
5'-isobutylthioinosine + phosphate
hypoxanthine + 5-isobutylthio-D-ribose 1-phosphate
show the reaction diagram
Caldariella acidophila
-
8.1% of the reaction with 5'-methylthioadenosine
-
-
?
5'-methylselenoadenosine + phosphate
adenine + 5-methylseleno-D-ribose 1-phosphate
show the reaction diagram
-
95% of the activity with 5'-methylthioadenosine
-
-
?
5'-methylthioadenosine + phosphate
adenine + 5-methylthio-D-ribose 1-phosphate
show the reaction diagram
5'-methylthioadenosine + phosphate
adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
show the reaction diagram
-
-
-
?
5'-methylthioinosine + phosphate
hypoxanthine + 5-methylthio-D-ribose 1-phosphate
show the reaction diagram
5'-n-butylthioadenosine + phosphate
adenine + 5-n-butylthio-D-ribose 1-phosphate
show the reaction diagram
Caldariella acidophila
-
93.3% of the reaction with 5'-methylthioadenosine
-
-
?
5'-n-propylthioadenosine + phosphate
adenine + 5-n-propylthio-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
5'-phenyladenosine + phosphate
adenine + 5-phenyl-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
5'-phenylthioadenosine + phosphate
adenine + 5-phenylthio-D-ribose 1-phosphate
show the reaction diagram
6-methylpurine 2'-deoxyribonucleoside + phosphate
6-methylpurine + 2-deoxy-D-ribose 1-phosphate
show the reaction diagram
-
substrate for trypanosomal but not for mammalian enzyme
-
-
?
9-[2-deoxy-beta-D-ribofuranosyl]-6-methylpurine + phosphate
?
show the reaction diagram
-
a toxic prodrug, 5% of the activity with S-methyl-5'-thioadenosine
-
-
?
9-[6-deoxy-beta-L-talofuranosyl]-2-F-adenine + phosphate
?
show the reaction diagram
-
a toxic prodrug, 23% of the activity with S-methyl-5'-thioadenosine
-
-
?
adenosine + phosphate
adenine + D-ribose 1-phosphate
show the reaction diagram
deoxyadenosine + phosphate
adenine + deoxy-D-ribose 1-phosphate
show the reaction diagram
-
-
-
-
?
guanosine + phosphate
guanine + D-ribose 1-phosphate
show the reaction diagram
inosine + phosphate
hypoxanthine + D-ribose 1-phosphate
show the reaction diagram
S-adenosyl-L-homocysteine + phosphate
?
show the reaction diagram
0.81% activity compared to activity with 5'-methylthioadenosine
-
-
?
S-adenosylhomocysteine + ?
?
show the reaction diagram
-
-
-
-
?
S-adenosylhomocysteine + phosphate
?
show the reaction diagram
14% relative activity
-
-
?
S-methyl-5'-thioadenosine + arsenate
adenine + S-methyl-5-thio-alpha-D-ribose 1-arsenate
show the reaction diagram
-
arsenolysis
-
-
?
S-methyl-5'-thioadenosine + phosphate
adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5'-deoxy-5'-(hydroxyethylthio)adenosine + phosphate
adenine + 5-hydroxyethylthio-D-ribose 1-phosphate
show the reaction diagram
5'-deoxy-5'-methylthioadenosine + phosphate
adenine + 5-methylthio-D-ribose 1-phosphate
show the reaction diagram
5'-methylthioadenosine + phosphate
adenine + 5-methylthio-D-ribose 1-phosphate
show the reaction diagram
adenosine + phosphate
adenine + D-ribose 1-phosphate
show the reaction diagram
guanosine + phosphate
guanine + D-ribose 1-phosphate
show the reaction diagram
P50389
-
-
-
-
inosine + phosphate
hypoxanthine + D-ribose 1-phosphate
show the reaction diagram
P50389
-
-
-
-
S-methyl-5'-thioadenosine + phosphate
adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(+/-)-cis-1-[(9-deazaadenin-9-yl)methyl]-4-ethyl-3-hydroxypyrrolidine
-
-
(+/-)-trans-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxy-4-(1H-1,2,3-triazol-4-yl)pyrrolidine
-
-
(+/-)-trans-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxy-4-(penta-3-yl)pyrrolidine
-
-
(+/-)-trans-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxy-4-isobutylpyrrolidine
-
-
(+/-)-trans-1-[(9-deazaadenin-9-yl)methyl]-4-ethyl-3-hydroxypyrrolidine
-
-
(+/-)-trans-1-[(9-deazaadenin-9-yl)methyl]-4-ethynyl-3-hydroxypyrrolidine
-
-
(+/-)-trans-4-(1-benzyl-1H-1,2,3-triazol-4-yl)-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxypyrrolidine
-
-
(+/-)-trans-4-(cyclohexylmethyl)-1-[(9-deaza-adenin-9-yl)methyl]-3-hydroxypyrrolidine
-
-
(+/-)-trans-4-allyl-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxypyrrolidine
-
-
(+/-)-trans-4-butyl-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxypyrrolidine
-
-
(+/-)-trans-4-cyclopropyl-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxypyrrolidine
-
-
(+/-)-trans-4-[3-(benzylthio)propyl]-1-[(9-deazaadenin-9-yl)-methyl]-3-hydroxypyrrolidine
-
-
(1S)-1-(9-deazaadenin-9-yl)-1,4-di-deoxy-1,4-imino-5-methylthio-D-ribitol
-
(1S)-1-(9-deazaadenin-9-yl)-1,4-dideoxy-1,4-imino-5-(1-naphthyl)thio-D-ribitol
-
-
(1S)-1-(9-deazaadenin-9-yl)-1,4-dideoxy-1,4-imino-5-(3-methylphenyl)thio-D-ribitol
-
-
(1S)-1-(9-deazaadenin-9-yl)-1,4-dideoxy-1,4-imino-5-(4-methylphenyl)thio-D-ribitol
-
-
(1S)-1-(9-deazaadenin-9-yl)-1,4-dideoxy-1,4-imino-5-methylthio-D-ribitol
-
-
(1S)-1-(9-deazaadenin-9-yl)-1,4-dideoxy-1,4-imino-5-O-methyl-D-ribitol
-
-
(1S)-1-(9-deazaadenin-9-yl)-1,4-dideoxy-1,4-imino-5-phenylthio-D-ribitol
-
-
(1S)-1-(9-deazaadenin-9-yl)-1,4-dideoxy-5-(2-fluoroethyl)thio-1,4-imino-D-ribitol
-
-
(1S)-1-(9-deazaadenin-9-yl)-1,4-dideoxy-5-(2-hydroxyethyl)thio-1,4-imino-D-ribitol
-
-
(1S)-1-(9-deazaadenin-9-yl)-1,4-dideoxy-5-(4-fluorophenyl)thio-1,4-imino-D-ribitol
-
-
(1S)-1-(9-deazaadenin-9-yl)-1,4-dideoxy-5-ethylthio-1,4-imino-D-ribitol
-
-
(1S)-1-(9-deazaadenin-9-yl)-1,4-dideoxy-5-n-propylthio-1,4-imino-D-ribitol
-
-
(1S)-1-(9-deazaadenin-9-yl)-1,4-imino-1,4,5,6,7-penta-deoxy-D-ribo-heptitol
-
-
(1S)-1-(9-deazaadenin-9-yl)-1,4-imino-1,4,5-trideoxy-D-ribitol
-
-
(1S)-1-(9-deazaadenin-9-yl)-5-(3-chlorophenyl)thio-1,4-dideoxy-1,4-imino-D-ribitol
-
-
(1S)-1-(9-deazaadenin-9-yl)-5-(4-chlorophenyl)thio-1,4-dideoxy-1,4-imino-D-ribitol
-
-
(1S)-1-(9-deazaadenin-9-yl)-5-benzylthio-1,4-dideoxy-1,4-imino-D-ribitol
-
-
(3R,4R)-1-[(9-deaza-adenin-9-yl)methyl]-3-hydroxy-4-methoxymethylpyrrolidine
-
-
(3R,4R)-4-(benzyloxymethyl)-1-[(9-deaza-adenin-9-yl)-methyl]-3-hydroxypyrrolidine
-
-
(3R,4S)-1-[(8-aza-9-deazaadenin-9-yl)methyl]-4-benzylthiomethyl-3-hydroxypyrrolidine hydrochloride
-
-
(3R,4S)-1-[(9-deaza-adenin-9-yl)methyl]-3-hydroxy-4-(1-propylthiomethyl)pyrrolidine
-
-
(3R,4S)-1-[(9-deaza-adenin-9-yl)methyl]-3-hydroxy-4-(2-propylthiomethyl)pyrrolidine
-
-
(3R,4S)-1-[(9-deaza-adenin-9-yl)methyl]-3-hydroxy-4-(4-pyridylthiomethyl)pyrrolidine
-
-
(3R,4S)-1-[(9-deaza-adenin-9-yl)methyl]-3-hydroxy-4-phenylthiomethylpyrrolidine
-
-
(3R,4S)-1-[(9-deaza-adenin-9-yl)methyl]-4-(4-fluorophenylthiomethyl)-3-hydroxypyrrolidine
-
-
(3R,4S)-1-[(9-deaza-adenin-9-yl)methyl]-4-ethylthiomethyl-3-hydroxypyrrolidine
-
-
(3R,4S)-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxy-4-(1-propyl)pyrrolidine
-
-
(3R,4S)-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxy-4-(4-chlorophe-nylthiomethyl)pyrrolidine
-
(3R,4S)-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxy-4-(methylthiomethyl)pyrrolidine
-
(3R,4S)-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxy-4-methylthiomethylpyrrolidine
-
-
(3R,4S)-1-[(9-deazaadenin-9-yl)methyl]-4-ethyl-3-hydroxypyrrolidine
-
-
(3R,4S)-1-[(9-deazaadenin-9yl)methyl]-3-hydroxy-4-(methylthio-methyl) pyrrolidine
-
treatment of cultured FaDu and Cal27 cells with 0.001 mM (3R,4S)-1-[(9-deazaadenin-9yl)methyl]-3-hydroxy-4-(methylthio-methyl) pyrrolidine and 0.02 mM 5'-methylthioadenosine inhibits MTAP by 96%, increases cellular 5'-methylthioadenosine concentrations, decreases polyamines, and induces apoptosis
(3R,4S)-4-(1-butylthiomethyl)-1-[(9-deaza-adenin-9-yl)-methyl]-3-hydroxypyrrolidine
-
-
(3R,4S)-4-(3-chlorophenylthiomethyl)-1-[(9-deaza-adenin-9-yl)methyl]-3-hydroxypyrrolidine
-
-
(3R,4S)-4-(4-chlorophenylthiomethyl)-1-[(9-deaza-adenin-9-yl)methyl]-3-hydroxypyrrolidine
-
-
(3R,4S)-4-benzylthiomethyl-1-[(9-deazaadenin-9-yl)-methyl]-3-hydroxypyrrolidine
-
-
(3R,4S)-4-butyl-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxypyrrolidine
-
-
(3R,4S)-4-cyclohexylthiomethyl-1-[(9-deaza-adenin-9-yl)methyl]-3-hydroxypyrrolidine
-
-
2'-deoxyguanosine
-
-
2'-deoxyinosine
-
weak, competitive
2-bromo-9-(1,3-dihydroxy-2-propoxymethyl)adenine
-
strong
2-bromo-9-(2-hydroxyethoxymethyl)adenine
-
-
2-chloro-9-(1,3-dihydroxy-2-propoxymethyl)adenine
-
strong
2-chloro-9-(2-hydroxyethoxymethyl)adenine
-
-
2-Deoxyribose 1-phosphate
-
-
2-iodo-9-(1,3-dihydroxy-2-propoxymethyl)adenine
-
strong
2-iodo-9-(2-hydroxyethoxymethyl)adenine
-
-
4-Cl-PhT-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin A
-
-
-
5'-butylthio-DADMe-ImmucillinA
5'-Chloroformycin
-
-
5'-Deoxy-5'-chloroformycin
-
competitive
5'-deoxy-5'-methylthiotubercidin
-
-
5'-deoxy-adenosine
-
and analogues; strong, competitive
5'-dimethylthioadenosine
5'-ethylthio-DADMe-ImmucillinA
-
inhibits quorum sensing induction completely at 0.001 mM
5'-ethylthioadenosine
-
-
5'-methylthio-DADMe-immucillin A
-
5'-methylthio-DaDMe-Immucillin-A
-
-
5'-methylthio-DADMe-ImmucillinA
5'-methylthio-immucillin-adenine
-
i.e. MT-immucillin-A
-
5'-methylthiotubercidin
5'-n-Propylthioadenosine
-
-
5-methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin A
-
-
-
5-methylthio-immucillin A
-
-
-
9-(phosphonoalkyl)adenine
-
-
-
9-(phosphonoheptyl)adenine
-
-
-
9-deazaadenine
-
-
-
9-[(1-Hydroxy-3-iodo-2-propoxy)methyl]adenine
adenine
adenine arabinoside
-
weak, competitive
adenosine
-
strong, competitive
calcineurin B-like 3
-
inhibits only in the presence of Ca2+, MTAN interacts specifically with calcineurin B-like 3, but not with calcineurin B-like 1 and calcineurin B-like 4. The 91-amino acid region of calcineurin B-like 3 is sufficient for the interaction with MTAN. Calcineurin B-like 3 and MTAN associate with each other in plant cells, they interact in vitro and in vivo, form a complex outside of the nucleus, colocalize mainly at the plasma membrane
-
dithiothreitol
-
0.8 M, reduction of thermostability
DTNB
-
-
Formycin A
fructose 1-phosphate
-
-
guanidine hydrochloride
-
only recombinant enzyme
guanine
guanosine
-
weak, competitive
hydrogen peroxide
Inosine
-
weak, competitive
iodoacetamide
iodoacetate
L-methionine
-
weak
MT-ImmA
-
i.e. (3R,4S)-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxy-4-methylthiomethylpyrrolidine
N-ethylmaleimide
-
5'-methylthioadenosine partly protects
p-chloromercuribenzoic acid
p-Cl-PhT-DADMe-ImmA
-
-
proteinase K
-
recombinant enzyme, 10% remaining activity after 4 h at 37°C, phosphate protects
-
ribose 1-phosphate
-
-
S-adenosyl-L-homocysteine
SH-group blocking compounds
-
Subtilisin
-
recombinant enzyme, 24% remaining activity after 4 h at 37°C, phosphate protects
-
tert-butyl hydroperoxide
tert-butyl trans-3-hydroxy-4-vinylpyrrolidine-1-carboxylate
-
-
trans-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxy-4-vinylpyrrolidine
-
-
trans-4-cyclopentyl-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxypyrrolidine
-
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dithiothreitol
putrescine
SH-group reducing agents
-
spermidine
-
increases activity
spermine
-
increases activity
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.032
2'-chloro-2'-deoxyadenosine
-
at pH 7.4 and 37°C
3.6
2'-deoxy-2'-azido-5'-deoxy-5'-methylthioadenosine
-
-
0.0031
2'-deoxy-5'-deoxy-5'-methylthioadenosine
-
-
0.005
2'-fluoro-2'-deoxyadenosine
-
at pH 7.4 and 37°C
-
0.0039
3'-deoxy-5'-deoxy-5'-methylthioadenosine
-
-
0.009
5'-deoxy-5'-(1,3-thiazol)-2-yl-sulfanyladenosine
-
-
0.005
5'-deoxy-5'-(2-(pyridin-2-yl)ethyl)-thioadenosine
-
-
0.0028
5'-deoxy-5'-(2-methylbutyl)-thioadenosine
-
-
0.002
5'-deoxy-5'-(2-methylphenyl)-thioadenosine
-
-
0.0015
5'-deoxy-5'-(3-methylphenyl)-thioadenosine
-
-
0.0032
5'-deoxy-5'-(4-methoxyphenyl)-thioadenosine
-
-
0.0017
5'-deoxy-5'-(4-methylphenyl)-thioadenosine
-
-
0.0046
5'-deoxy-5'-(phenylethyl)-thioadenosine
-
-
0.0034
5'-deoxy-5'-(phenylmethyl)-thioadenosine
-
-
0.0052
5'-deoxy-5'-(quinolin-2-yl)-sulfanyladenosine
-
-
0.0031
5'-deoxy-5'-butylthioadenosine
-
-
0.00102
5'-deoxy-5'-cyclohexyl-thioadenosine
-
-
0.0035
5'-deoxy-5'-cyclopentyl-thioadenosine
-
-
0.03
5'-deoxy-5'-ethylthioadenosine
-
-
0.004
5'-deoxy-5'-hydroxyethylthioadenosine
-
-
0.003
5'-deoxy-5'-imidazol-2-yl-sulfanyladenosine
-
-
0.0018
5'-deoxy-5'-isobutylthioadenosine
-
-
0.017
5'-deoxy-5'-isopentylthioadenosine
-
-
0.0018
5'-deoxy-5'-methyl-thioadenosine
-
-
0.00001 - 0.026
5'-deoxy-5'-methylthioadenosine
0.0069
5'-deoxy-5'-phenyl-thioadenosine
-
-
0.00088
5'-deoxy-5'-pyridin-2-yl-sulfanyladenosine
-
-
0.005
5'-deoxy-5'-pyridin-4-yl-sulfanyladenosine
-
-
0.025
5'-deoxy-5'-tert-butylthioadenosine
-
-
0.007
5'-deoxy-5'-[4-(trifluoromethyl)phenyl]-thioadenosine
-
-
0.006
5'-deoxy-adenosine
-
-
0.0043 - 0.023
5'-deoxyadenosine
0.00047 - 0.3
5'-methylthioadenosine
0.008
5-methylthioribose 1-phosphate
-
-
0.023
adenine
-
-
0.013 - 0.184
adenosine
0.0083
deoxyadenosine
-
at pH 7.4 and 37°C
0.1136 - 0.916
guanosine
0.084 - 0.963
Inosine
0.123 - 13.5
phosphate
0.2009
S-adenosylhomocysteine
-
-
0.00009 - 8
S-methyl-5'-thioadenosine
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6.4
2'-chloro-2'-deoxyadenosine
Trypanosoma brucei
-
at pH 7.4 and 37°C
0.04
2'-deoxy-2'-azido-5'-deoxy-5'-methylthioadenosine
Homo sapiens
-
-
0.0004
2'-deoxy-2-fluoro-5'-deoxy-5'-methylthioadenosine
Homo sapiens
-
-
0.28
2'-deoxy-5'-deoxy-5'-methylthioadenosine
Homo sapiens
-
-
5.4
2'-fluoro-2'-deoxyadenosine
Trypanosoma brucei
-
at pH 7.4 and 37°C
-
0.004
3'-deoxy-5'-deoxy-5'-methylthioadenosine
Homo sapiens
-
-
3.8
5'-deoxy-5'-(1,3-thiazol)-2-yl-sulfanyladenosine
Homo sapiens
-
-
2.14
5'-deoxy-5'-(2-(pyridin-2-yl)ethyl)-thioadenosine
Homo sapiens
-
-
5.5
5'-deoxy-5'-(2-methylbutyl)-thioadenosine
Homo sapiens
-
-
2.4
5'-deoxy-5'-(2-methylphenyl)-thioadenosine
Homo sapiens
-
-
1.4
5'-deoxy-5'-(3-methylphenyl)-thioadenosine
Homo sapiens
-
-
1.5
5'-deoxy-5'-(4-methoxyphenyl)-thioadenosine
Homo sapiens
-
-
0.3
5'-deoxy-5'-(4-methylphenyl)-thioadenosine
Homo sapiens
-
-
2.5
5'-deoxy-5'-(phenylethyl)-thioadenosine
Homo sapiens
-
-
2.49
5'-deoxy-5'-(phenylmethyl)-thioadenosine
Homo sapiens
-
-
3.44
5'-deoxy-5'-(quinolin-2-yl)-sulfanyladenosine
Homo sapiens
-
-
2.3
5'-deoxy-5'-butylthioadenosine
Homo sapiens
-
-
1.01
5'-deoxy-5'-cyclohexyl-thioadenosine
Homo sapiens
-
-
1.57
5'-deoxy-5'-cyclopentyl-thioadenosine
Homo sapiens
-
-
0.67
5'-deoxy-5'-ethylthioadenosine
Homo sapiens
-
-
5.9
5'-deoxy-5'-hydroxyethylthioadenosine
Homo sapiens
-
-
1.5
5'-deoxy-5'-imidazol-2-yl-sulfanyladenosine
Homo sapiens
-
-
2.9
5'-deoxy-5'-isobutylthioadenosine
Homo sapiens
-
-
0.85
5'-deoxy-5'-isopentylthioadenosine
Homo sapiens
-
-
4.6
5'-deoxy-5'-methyl-thioadenosine
Homo sapiens
-
-
3.16
5'-deoxy-5'-phenyl-thioadenosine
Homo sapiens
-
-
0.23
5'-deoxy-5'-pyridin-2-yl-sulfanyladenosine
Homo sapiens
-
-
4.2
5'-deoxy-5'-pyridin-4-yl-sulfanyladenosine
Homo sapiens
-
-
1.46
5'-deoxy-5'-tert-butylthioadenosine
Homo sapiens
-
-
0.8
5'-deoxy-5'-[4-(trifluoromethyl)phenyl]-thioadenosine
Homo sapiens
-
-
4.8
5'-deoxy-adenosine
Homo sapiens
-
-
0.4 - 24.5
5'-methylthioadenosine
8.4 - 43.6
adenosine
9.6
deoxyadenosine
Trypanosoma brucei
-
at pH 7.4 and 37°C
7.31 - 29.6
guanosine
9.38 - 132
Inosine
0.26
phosphate
Mycobacterium tuberculosis
O06401
pH and temperature not specified in the publication, co-substrate: 0.2 mM 5'-methylthioadenosine
0.51 - 8.8
S-methyl-5'-thioadenosine
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
200
2'-chloro-2'-deoxyadenosine
Trypanosoma brucei
-
at pH 7.4 and 37°C
166450
1080
2'-fluoro-2'-deoxyadenosine
Trypanosoma brucei
-
at pH 7.4 and 37°C
210496
588 - 2600
5'-methylthioadenosine
400
650
adenosine
Trypanosoma brucei
-
at pH 7.4 and 37°C
122
1160
deoxyadenosine
Trypanosoma brucei
-
at pH 7.4 and 37°C
601
15 - 24000
S-methyl-5'-thioadenosine
1578
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0000018
(+/-)-cis-1-[(9-deazaadenin-9-yl)methyl]-4-ethyl-3-hydroxypyrrolidine
-
pH 7.4, 25°C
0.000002
(+/-)-trans-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxy-4-(1H-1,2,3-triazol-4-yl)pyrrolidine
-
pH 7.4, 25°C
0.0000007
(+/-)-trans-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxy-4-(penta-3-yl)pyrrolidine
-
pH 7.4, 25°C
0.000000047
(+/-)-trans-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxy-4-isobutylpyrrolidine
-
pH 7.4, 25°C
0.00000084
(+/-)-trans-1-[(9-deazaadenin-9-yl)methyl]-4-ethyl-3-hydroxypyrrolidine
-
pH 7.4, 25°C
0.00000039
(+/-)-trans-1-[(9-deazaadenin-9-yl)methyl]-4-ethynyl-3-hydroxypyrrolidine
-
pH 7.4, 25°C
0.000000064
(+/-)-trans-4-(1-benzyl-1H-1,2,3-triazol-4-yl)-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxypyrrolidine
-
pH 7.4, 25°C
0.000000059
(+/-)-trans-4-(cyclohexylmethyl)-1-[(9-deaza-adenin-9-yl)methyl]-3-hydroxypyrrolidine
-
pH 7.4, 25°C
0.00000035
(+/-)-trans-4-allyl-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxypyrrolidine
-
pH 7.4, 25°C
0.000000009
(+/-)-trans-4-butyl-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxypyrrolidine
-
pH 7.4, 25°C
0.000000063
(+/-)-trans-4-cyclopropyl-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxypyrrolidine
-
pH 7.4, 25°C
0.000000054
(+/-)-trans-4-[3-(benzylthio)propyl]-1-[(9-deazaadenin-9-yl)-methyl]-3-hydroxypyrrolidine
-
pH 7.4, 25°C
0.000026
(1S)-1-(9-deazaadenin-9-yl)-1,4-di-deoxy-1,4-imino-5-methylthio-D-ribitol
pH 7.0, 25°C
0.00009
(1S)-1-(9-deazaadenin-9-yl)-1,4-dideoxy-1,4-imino-5-(1-naphthyl)thio-D-ribitol
-
pH 7.4, 25°C
0.00000139
(1S)-1-(9-deazaadenin-9-yl)-1,4-dideoxy-1,4-imino-5-(3-methylphenyl)thio-D-ribitol
-
pH 7.4, 25°C
0.0000044
(1S)-1-(9-deazaadenin-9-yl)-1,4-dideoxy-1,4-imino-5-(4-methylphenyl)thio-D-ribitol
-
pH 7.4, 25°C
0.000026
(1S)-1-(9-deazaadenin-9-yl)-1,4-dideoxy-1,4-imino-5-methylthio-D-ribitol
-
pH 7.4, 25°C
0.000134
(1S)-1-(9-deazaadenin-9-yl)-1,4-dideoxy-1,4-imino-5-O-methyl-D-ribitol
-
pH 7.4, 25°C
0.0000036
(1S)-1-(9-deazaadenin-9-yl)-1,4-dideoxy-1,4-imino-5-phenylthio-D-ribitol
-
pH 7.4, 25°C
0.00002
(1S)-1-(9-deazaadenin-9-yl)-1,4-dideoxy-5-(2-fluoroethyl)thio-1,4-imino-D-ribitol
-
pH 7.4, 25°C
0.000056
(1S)-1-(9-deazaadenin-9-yl)-1,4-dideoxy-5-(2-hydroxyethyl)thio-1,4-imino-D-ribitol
-
pH 7.4, 25°C
0.0000064
(1S)-1-(9-deazaadenin-9-yl)-1,4-dideoxy-5-(4-fluorophenyl)thio-1,4-imino-D-ribitol
-
pH 7.4, 25°C
0.0000092
(1S)-1-(9-deazaadenin-9-yl)-1,4-dideoxy-5-ethylthio-1,4-imino-D-ribitol
-
pH 7.4, 25°C
0.000004
(1S)-1-(9-deazaadenin-9-yl)-1,4-dideoxy-5-n-propylthio-1,4-imino-D-ribitol
-
pH 7.4, 25°C
0.000044
(1S)-1-(9-deazaadenin-9-yl)-1,4-imino-1,4,5,6,7-penta-deoxy-D-ribo-heptitol
-
pH 7.4, 25°C
0.00072
(1S)-1-(9-deazaadenin-9-yl)-1,4-imino-1,4,5-trideoxy-D-ribitol
-
pH 7.4, 25°C
0.0000064
(1S)-1-(9-deazaadenin-9-yl)-5-(3-chlorophenyl)thio-1,4-dideoxy-1,4-imino-D-ribitol
-
pH 7.4, 25°C
0.000000576
(1S)-1-(9-deazaadenin-9-yl)-5-(4-chlorophenyl)thio-1,4-dideoxy-1,4-imino-D-ribitol
-
pH 7.4, 25°C
0.000026
(1S)-1-(9-deazaadenin-9-yl)-5-benzylthio-1,4-dideoxy-1,4-imino-D-ribitol
-
pH 7.4, 25°C
0.000085
(3R,4R)-1-[(9-deaza-adenin-9-yl)methyl]-3-hydroxy-4-methoxymethylpyrrolidine
-
pH 7.5, 25°C
0.000022
(3R,4R)-4-(benzyloxymethyl)-1-[(9-deaza-adenin-9-yl)-methyl]-3-hydroxypyrrolidine
-
pH 7.5, 25°C
0.000055
(3R,4S)-1-[(8-aza-9-deazaadenin-9-yl)methyl]-4-benzylthiomethyl-3-hydroxypyrrolidine hydrochloride
-
pH 7.5, 25°C
0.0000013
(3R,4S)-1-[(9-deaza-adenin-9-yl)methyl]-3-hydroxy-4-(1-propylthiomethyl)pyrrolidine
-
pH 7.5, 25°C
0.0000009
(3R,4S)-1-[(9-deaza-adenin-9-yl)methyl]-3-hydroxy-4-(2-propylthiomethyl)pyrrolidine
-
pH 7.5, 25°C
0.000014
(3R,4S)-1-[(9-deaza-adenin-9-yl)methyl]-3-hydroxy-4-(4-pyridylthiomethyl)pyrrolidine
-
pH 7.5, 25°C
0.0000015
(3R,4S)-1-[(9-deaza-adenin-9-yl)methyl]-3-hydroxy-4-phenylthiomethylpyrrolidine
-
pH 7.5, 25°C
0.000002
(3R,4S)-1-[(9-deaza-adenin-9-yl)methyl]-4-(4-fluorophenylthiomethyl)-3-hydroxypyrrolidine
-
pH 7.5, 25°C
0.00000065
(3R,4S)-1-[(9-deaza-adenin-9-yl)methyl]-4-ethylthiomethyl-3-hydroxypyrrolidine
-
pH 7.5, 25°C
0.002
(3R,4S)-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxy-4-(1-propyl)pyrrolidine
-
above, pH 7.5, 25°C
0.00000036 - 0.00000175
(3R,4S)-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxy-4-(methylthiomethyl)pyrrolidine
0.0000017
(3R,4S)-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxy-4-methylthiomethylpyrrolidine
-
pH 7.5, 25°C
0.00000031
(3R,4S)-1-[(9-deazaadenin-9-yl)methyl]-4-ethyl-3-hydroxypyrrolidine
-
pH 7.4, 25°C
0.00000028
(3R,4S)-4-(1-butylthiomethyl)-1-[(9-deaza-adenin-9-yl)-methyl]-3-hydroxypyrrolidine
-
pH 7.5, 25°C
0.00000081
(3R,4S)-4-(3-chlorophenylthiomethyl)-1-[(9-deaza-adenin-9-yl)methyl]-3-hydroxypyrrolidine
-
pH 7.5, 25°C
0.000002
(3R,4S)-4-(4-chlorophenylthiomethyl)-1-[(9-deaza-adenin-9-yl)methyl]-3-hydroxypyrrolidine
-
pH 7.5, 25°C
0.0000014
(3R,4S)-4-benzylthiomethyl-1-[(9-deazaadenin-9-yl)-methyl]-3-hydroxypyrrolidine
-
pH 7.5, 25°C
0.0000000034
(3R,4S)-4-butyl-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxypyrrolidine
-
pH 7.4, 25°C
0.00000076
(3R,4S)-4-cyclohexylthiomethyl-1-[(9-deaza-adenin-9-yl)methyl]-3-hydroxypyrrolidine
-
pH 7.5, 25°C
0.0002 - 0.0007
2-bromo-9-(1,3-dihydroxy-2-propoxymethyl)adenine
-
-
0.001 - 0.01
2-bromo-9-(2-hydroxyethoxymethyl)adenine
-
-
0.0002 - 0.0007
2-chloro-9-(1,3-dihydroxy-2-propoxymethyl)adenine
-
-
0.001 - 0.01
2-chloro-9-(2-hydroxyethoxymethyl)adenine
-
-
0.0002 - 0.0007
2-iodo-9-(1,3-dihydroxy-2-propoxymethyl)adenine
-
-
0.001 - 0.01
2-iodo-9-(2-hydroxyethoxymethyl)adenine
-
-
0.0083 - 0.031
5'-methylthiotubercidin
0.015
9-(phosphonoheptyl)adenine
-
low phosphate concentration of 3.5 mM
-
0.172
adenine
-
-
0.048
Formycin A
-
at 0.05 mM
0.000026
MT-ImmA
-
pH 7.5, 25°C
0.00000003
tert-butyl trans-3-hydroxy-4-vinylpyrrolidine-1-carboxylate
-
pH 7.4, 25°C
0.00000065
trans-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxy-4-vinylpyrrolidine
-
pH 7.4, 25°C
0.000000013
trans-4-cyclopentyl-1-[(9-deazaadenin-9-yl)methyl]-3-hydroxypyrrolidine
-
pH 7.4, 25°C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00000001 - 0.000125
5'-butylthio-DADMe-ImmucillinA
0.00000011 - 0.00000014
5'-ethylthio-DADMe-ImmucillinA
0.000025 - 0.000075
5'-methylthio-DADMe-immucillin A
0.000000105 - 0.0006
5'-methylthio-DADMe-ImmucillinA
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0234
-
purified enzyme
0.0283
-
partially purified enzyme
0.048
-
substrate 2-amino-5'-deoxy-5'-(hydroxyethylthio)adenosine
0.092
-
partially purified enzyme, forward reaction, substrate 5'-methylthioadenosine
0.131
-
purified enzyme
0.17
-
substrate 5'-methyladenosine or 5'-deoxy-5'-(hydroxyethylthio)adenosine
0.25
-
substrate 2-fluoro-5'-deoxy-5'-(hydroxyethylthio)adenosine
0.26
-
substrate 5'-deoxyadenosine, forward reaction
0.365
-
substrate 5'-deoxy-5'-(hydroxyethylthio)adenosine, preincubation with substrate
0.38
-
with adenosine as substrate
0.46
-
with 5'-choroadenosine as substrate
0.67
-
purified enzyme
0.83
-
with 5'-choroadenosine as substrate
0.96
-
with S-adenosylhomocysteine as substrate
1.06
-
with 5'-iodoadenosine as substrate
1.36
-
with 5'-phenylthioadenosine as substrate
1.5
-
with 5'-butylthioadenosine as substrate
1.85
-
with 5'-isobutylthioadenosine as substrate
1.98
-
with 5'-isobutylthioadenosine as substrate
2.44
-
with 5'-phenylthioadenosine as substrate
2.46
Caldariella acidophila
-
purified enzyme
2.48
Caldariella acidophila
-
purified enzyme
2.6
-
with 5'-ethylthioadenosine as substrate
2.72
-
with 5'-butylthioadenosine as substrate
3.14
-
with 5'-iodoadenosine as substrate
4.42
-
with 5'-ethylthioadenosine as substrate
4.86
-
70°C, pH 7.4, mutant enzyme C259S/C261S/C262S/C200S/C205S
4.97
-
70°C, pH 7.4, mutant enzyme C262S
5.09
-
70°C, pH 7.4, mutant enzyme C259S/C261S
5.28
-
70°C, pH 7.4, mutant enzyme C259S/C261S/C262S/C200S/C205S/C138S/C164S
5.68
-
70°C, pH 7.4, mutant enzyme C259S/C261S/C262S
6.24
-
70°C, pH 7.4, mutant enzyme C259S/C261S/C262S/C200S/C205S/C138S
6.25
-
with 5'-methylthioadenosine as substrate
6.27
-
70°C, pH 7.4, wild-type enzyme
6.35
-
purified enzyme
9.5
-
purified recombinant enzyme
10.2
-
purified enzyme
10.3
-
-
11.5
-
with 5'-methylthioadenosine as substrate
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.1 - 7.6
-
-
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9
Caldariella acidophila
-
50% of activity maximum at pH 6.0 and pH 9.0
6.2 - 8.6
-
50% of activity maximum at pH 6.2 and pH 8.6
6.5 - 9
pH 6.5: about 55% of maximal activity, pH 9.0: about 55% of maximal activity
6.5 - 8.5
-
considerable decrease of activity below pH 6.5 and above pH 8.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
assay at
95
Caldariella acidophila
-
-
100
-
recombinant enzyme; recombinant enzyme
107
-
mutant enzyme C259S/C261S/C262S/C200S/C205S/C164S
112
-
mutant enzyme C259S/C261S/C262S/C200S/C205S
114
-
mutant enzyme C259S/C261S/C262S/C200S/C205S and mutant enzyme C259S/C261S/C262S
115
-
mutant enzyme C259S/C261S and mutant enzyme C259S
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 120
-
recombinant enzyme
60 - 120
-
60°C: about 40% of maximal activity, 120°C: about 40% of maximal activity, recombinant enzyme
86 - 95
90 - 130
-
90°C: about 50% of maximal activity, 130°C: about 70% of maximal activity
95 - 140
-
95°C: about 50% of maximal activity, 140°C: about 45% of maximal activity, wild-type enzyme
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.7
-
isoelectric focusing on polyacrylamide gel in the pH ranges 3-10 and 4.5-6.0
6.54
-
calculation from nucleotide sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
MTAP is not detectable in 39% of chordomas
Manually annotated by BRENDA team
-
diffuse histiocytic lymphoma cell line, possesses no enzyme activity, but intact gene
Manually annotated by BRENDA team
-
highest level
Manually annotated by BRENDA team
-
and progenitor cells
Manually annotated by BRENDA team
-
high expression in activated hepatic stellate cells
Manually annotated by BRENDA team
-
cell line derived from metastases of malignant melanomas
Manually annotated by BRENDA team
-
tumor-derived cell line, bladder carcinoma
Manually annotated by BRENDA team
-
peripheral blood
Manually annotated by BRENDA team
-
diffuse, histiocytic
Manually annotated by BRENDA team
-
cell line derived from primary cutaneous melanoma
Manually annotated by BRENDA team
-
cell line derived from metastases of malignant melanomas
Manually annotated by BRENDA team
-
cell line derived from metastases of malignant melanomas
Manually annotated by BRENDA team
-
cell line derived from primary cutaneous melanoma
Manually annotated by BRENDA team
-
cell line derived from primary cutaneous melanoma; low content
Manually annotated by BRENDA team
-
cell line derived from primary cutaneous melanoma; low content
Manually annotated by BRENDA team
-
primary
Manually annotated by BRENDA team
-
SK-Ml-28 cell line derived from metastases of malignant melanomas
Manually annotated by BRENDA team
-
lung fibroblast cell line
Manually annotated by BRENDA team
-
decreased or no expression
Manually annotated by BRENDA team
-
CD34+ erythroid burst-forming unit, granulocyte/monocyte colony-forming unit, granulocyte/erythrocyte/macrophage/megakaryocyte colony-forming unit progenitors and primitive high proliferative potential colony-forming cells in the purified CD34+ cells are cultured
Manually annotated by BRENDA team
-
tumor-derived cell line, Burkitt's lymphoma
Manually annotated by BRENDA team
-
tumor-derived cell line, neuroblastoma
Manually annotated by BRENDA team
-
FC1010 skin fibroblast cell line
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
cytoplasmic MTAP is not detectable in 39% of chordomas
Manually annotated by BRENDA team
additional information
-
localized at the plasma membrane, in the cytoplasm and in the nucleus of transgenic tobacco plants. Abundant in the membrane structure and weak in the cytoplasm of onion epidermal cells
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
26000
-
x * 26000, calculated from sequence
27460
-
sequence analysis
28450
-
sequence analysis
30300
-
2 * 30300, SDS-PAGE
31000
-
x * 31000, SDS-PAGE
32500
-
3 * 32500, SDS-PAGE with addition of 8 M urea and 1% 2-mercaptoethanol
34000
-
x * 34000, SDS-PAGE
54000
-
gel filtration
55000
-
gel filtration
64000
-
gel filtration
89000
-
gel filtration
97000
gel filtration
160000
180000