Cloned (Comment) | Organism |
---|---|
expression at a high level in Escherichia coli | Saccharolobus solfataricus |
overexpression in Escherichia coli strain RB791, amino acid determination, incorrect positioning of disulfide bonds, the recombinant enzyme is less thermostable and thermophilic than the native enzyme | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
guanidine hydrochloride | only recombinant enzyme | Saccharolobus solfataricus | |
iodoacetamide | only recombinant enzyme | Saccharolobus solfataricus | |
iodoacetate | only recombinant enzyme | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
5'-methylthioadenosine | recombinant enzyme | Saccharolobus solfataricus | |
0.024 | - |
5'-methylthioadenosine | wild-type and recombinant enzyme | Saccharolobus solfataricus | |
0.125 | - |
phosphate | recombinant enzyme | Saccharolobus solfataricus | |
0.125 | - |
phosphate | wild-type and recombinant enzyme | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
6 * 27000, recombinant enzyme, SDS-PAGE | Saccharolobus solfataricus |
160000 | - |
gel filtration | Saccharolobus solfataricus |
160000 | - |
recombinant enzyme, gel filtration | Saccharolobus solfataricus |
160000 | - |
recombinant enzyme | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus | P50389 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Saccharolobus solfataricus |
recombinant from Escherichia coli | Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.115 | - |
- |
Saccharolobus solfataricus |
2.12 | - |
purified recombinant enzyme | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-methylthioadenosine + phosphate | - |
Saccharolobus solfataricus | adenine + 5-methylthio-D-ribose 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 27000, recombinant enzyme, SDS-PAGE | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
5'-methylthioadenosine phosphorylase | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Saccharolobus solfataricus |
100 | - |
recombinant enzyme | Saccharolobus solfataricus |
120 | - |
wild-type enzyme | Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 120 | recombinant enzyme | Saccharolobus solfataricus |
60 | 120 | 60°C: about 40% of maximal activity, 120°C: about 40% of maximal activity, recombinant enzyme | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the recombinant enzyme, expressed in Escherichia coli, is less thermostable and thermophilic than the native enzyme due to incorrect positioning of disulfide bonds | Saccharolobus solfataricus |
additional information | - |
the recombinant 5'-methylthioadenosine phosphorylase is less thermophilic and thermostable than the Sulfolobus solfataricus enzyme, since an incorrect positioning of disulfide bonds within the molecule generates structures less stable to thermal unfolding | Saccharolobus solfataricus |
70 | - |
recombinant enzyme, 0.8 M DTT, stable | Saccharolobus solfataricus |
90 | - |
recombinant enzyme, 0.8 M DTT, 2 h, loss of 38% activity | Saccharolobus solfataricus |
100 | - |
recombinant enzyme, 1 h, 85% remaining activity | Saccharolobus solfataricus |
100 | - |
1 h, 15% loss of activity of recombinant enzyme, wild-type enzyme remains stable | Saccharolobus solfataricus |
111 | - |
recombinant enzyme, melting temperature | Saccharolobus solfataricus |
111 | - |
melting temperature of recombinant enzyme | Saccharolobus solfataricus |
118 | - |
recombinant enzyme in presence of 100 mM phosphate, melting temperature | Saccharolobus solfataricus |