Cloned (Comment) | Organism |
---|---|
recombinant SsMTAPII and its C262S and C259S/C261S mutant forms are expressed in Escherichia coli BL21 (lDE3) cells | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
C259S/C261S | in contrast wo wild-type C262S and C259S/C261S mutants show complete thermal denaturation curves with sigmoidal transitions centered at 102°C and 99°C respectively. Under reducing conditions these values decrease by 4°C and 8°C respectively, highlighting the important role exerted by the CXC disulfide on enzyme thermostability. The double mutant (the mutant lacking the structural CXC motif), has more impact on the thermostability of SsMTAPII than the single mutant | Saccharolobus solfataricus |
C262S | in contrast wo wild-type C262S and C259S/C261S mutants show complete thermal denaturation curves with sigmoidal transitions centered at 102°C and 99°C respectively. Under reducing conditions these values decrease by 4°C and 8°C respectively, highlighting the important role exerted by the CXC disulfide on enzyme thermostability | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using heat-treatment and MTA-Sepharose chromatography | Saccharolobus solfataricus |
Subunits | Comment | Organism |
---|---|---|
hexamer | - |
Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
5'-deoxy-5'-methylthioadenosine phosphorylase II | - |
Saccharolobus solfataricus |